Physiological and Transcriptional Analyses Reveal Differential Phytohormone Responses to Boron Deficiency in Brassica napus Genotypes

In areas where soils are low in boron (B), the ability to predict B deficiency by plant analysis depends on the development of calibrated standards and offers the potential of preventing or minimising losses in seed yield. The present study aimed at establishing relationships between seed yield in winter oilseed rape (Brassica napus) and B concentrations in defined leaves during growth before fiowering and from them to determine critical values for the prognosis of B deficiency. Oilseed rape cv. Zheyouyou 2 was grown in 10 field experiments in Zhejiang province, south-east China, with B rates from 0 to 3· 3 kg/ha. At 4 of the sites, oilseed rape was resown in the following year to assess seasonal effects on critical concentrations. Experiments were conducted on the 3 main soil groups on which oilseed rape is grown in south-east China. The youngest open leaf (YOL) was selected as the sampling part because its B concentrations were strongly responsive to increasing B supply and well correlated with seed yield response. In this study, the best model of the relationship between YOL B concentration and yield was the Mitscherlich equation, which explained 68-89% of the variation in the data. The calculated critical range in the YOL at seedling stage was 20-25 mg B/kg and appeared to be valid in 2 seasons and on 3 soil types. The critical B range for prognosis of B deficiency was found to predict consistently those crops in farmers" fields that had low seed yield and low soil B, but needs to be evaluated in other growing environments especially those for spring rape.

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Prognosis of Boron Deficiency in Oilseed Rape (Brassica napus) by Soil Analysis

Boron in Plant and Animal Nutrition ◽

10.1007/978-1-4615-0607-2_30 ◽

2002 ◽

pp. 311-317

Author(s):

Youzhang Wei ◽

Richard W. Bell ◽

Yuai Yang

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Soil Analysis ◽

Boron Deficiency

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The importance of sampling immature leaves for the diagnosis of boron deficiency in oilseed rape (Brassica napus cv. Eureka)

Plant and Soil ◽

10.1007/bf00011434 ◽

1996 ◽

Vol 183 (2) ◽

pp. 187-198 ◽

Cited By ~ 29

Author(s):

Longbin Huang ◽

Zhengqian Ye ◽

Richard W. Bell

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Boron Deficiency

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Proline metabolism and biosynthesis behave differently in response to boron-deficiency and toxicity in Brassica napus

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2021.08.029 ◽

2021 ◽

Vol 167 ◽

pp. 529-540

Author(s):

Lei Yan ◽

Shuang Li ◽

Muhammad Riaz ◽

Cuncang Jiang

Keyword(s):

Brassica Napus ◽

Boron Deficiency ◽

Proline Metabolism

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Differential Alternative Splicing Genes in Response to Boron Deficiency in Brassica napus

Genes ◽

10.3390/genes10030224 ◽

2019 ◽

Vol 10 (3) ◽

pp. 224 ◽

Cited By ~ 2

Author(s):

Jin Gu ◽

Wei Li ◽

Sheliang Wang ◽

Xiaoyan Zhang ◽

Anne Coules ◽

...

Keyword(s):

Alternative Splicing ◽

Brassica Napus ◽

Alternative Splice ◽

Oilseed Rape ◽

Splice Site ◽

Intron Retention ◽

Boron Deficiency ◽

Plant Responses ◽

Plant Tolerance ◽

Alternative Splice Site

Alternative splicing (AS) can increase transcriptome diversity, protein diversity and protein yield, and is an important mechanism to regulate plant responses to stress. Oilseed rape (Brassica napus L.), one of the main oil crops in China, shows higher sensitivity to boron (B) deficiency than other species. Here, we demonstrated AS changes that largely increased the diversity of the mRNA expressed in response to B deficiency in B. napus. Each gene had two or more transcripts on average. A total of 33.3% genes in both Qingyou10 (QY10, B-efficient cultivar) and Westar10 (W10, B-inefficient cultivar) showed AS in both B conditions. The types of AS events were mainly intron retention, 3′ alternative splice site, 5′ alternative splice site and exon skipping. The tolerance ability of QY10 was higher than that of W10, possibly because there were far more differential alternative splicing (DAS) genes identified in QY10 at low B conditions than in W10. The number of genes with both DAS and differentially expressed (DE) was far lower than that of the genes that were either with DAS or DE in QY10 and W10, suggesting that the DAS and DE genes were independent. Four Serine/Arginine-rich (SR) splicing factors, BnaC06g14780D, BnaA01g14750D, BnaA06g15930D and BnaC01g41640D, underwent differentially alternative splicing in both cultivars. There existed gene–gene interactions between BnaC06g14780D and the genes associated with the function of B in oilseed rape at low B supply. This suggests that oilseed rape could regulate the alterative pre-mRNA splicing of SR protein related genes to increase the plant tolerance to B deficiency.

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Genetic variation of BnaA3.NIP5;1 expressing in the lateral root cap contributes to boron deficiency tolerance in Brassica napus

PLoS Genetics ◽

10.1371/journal.pgen.1009661 ◽

2021 ◽

Vol 17 (7) ◽

pp. e1009661

Author(s):

Mingliang He ◽

Sheliang Wang ◽

Cheng Zhang ◽

Liu Liu ◽

Jinyao Zhang ◽

...

Keyword(s):

Genetic Variation ◽

Brassica Napus ◽

Seed Yield ◽

Lateral Root ◽

Natural Populations ◽

Field Tests ◽

Root Cap ◽

Boron Deficiency ◽

Root Tips ◽

Transgenic Lines

Boron (B) is essential for vascular plants. Rapeseed (Brassica napus) is the second leading crop source for vegetable oil worldwide, but its production is critically dependent on B supplies. BnaA3.NIP5;1 was identified as a B-efficient candidate gene in B. napus in our previous QTL fine mapping. However, the molecular mechanism through which this gene improves low-B tolerance remains elusive. Here, we report genetic variation in BnaA3.NIP5;1 gene, which encodes a boric acid channel, is a key determinant of low-B tolerance in B. napus. Transgenic lines with increased BnaA3.NIP5;1 expression exhibited improved low-B tolerance in both the seedling and maturity stages. BnaA3.NIP5;1 is preferentially polar-localized in the distal plasma membrane of lateral root cap (LRC) cells and transports B into the root tips to promote root growth under B-deficiency conditions. Further analysis revealed that a CTTTC tandem repeat in the 5’UTR of BnaA3.NIP5;1 altered the expression level of the gene, which is tightly associated with plant growth and seed yield. Field tests with natural populations and near-isogenic lines (NILs) confirmed that the varieties carried BnaA3.NIP5;1Q allele significantly improved seed yield. Taken together, our results provide novel insights into the low-B tolerance of B. napus, and the elite allele of BnaA3.NIP5;1 could serve as a direct target for breeding low-B-tolerant cultivars.

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