scholarly journals Yellow Mosaic Disease (YMD) of Mungbean (Vigna radiata (L.) Wilczek): Current Status and Management Opportunities

2020 ◽  
Vol 11 ◽  
Author(s):  
Gyan P. Mishra ◽  
Harsh K. Dikshit ◽  
Ramesh S. V. ◽  
Kuldeep Tripathi ◽  
Ranjeet R. Kumar ◽  
...  
Keyword(s):  
Vigna Radiata ◽  
Mosaic Disease ◽  
Current Status ◽  
Yellow Mosaic Disease

scholarly journals Corrigendum: Yellow Mosaic Disease (YMD) of Mungbean (Vigna radiata (L.) Wilczek): Current Status and Management Opportunities

2020 ◽  
Vol 11 ◽  
Author(s):  
Gyan P. Mishra ◽  
Harsh K. Dikshit ◽  
Ramesh S. V. ◽  
Kuldeep Tripathi ◽  
Ranjeet R. Kumar ◽  
...  
Keyword(s):  
Vigna Radiata ◽  
Mosaic Disease ◽  
Current Status ◽  
Yellow Mosaic Disease

scholarly journals Association Mapping for Yield Attributing Traits and Yellow Mosaic Disease Resistance in Mung Bean [Vigna radiata (L.) Wilczek]

2022 ◽  
Vol 12 ◽  
Author(s):  
Versha Rohilla ◽  
Rajesh Kumar Yadav ◽  
Atman Poonia ◽  
Ravika Sheoran ◽  
Gita Kumari ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Association Mapping ◽  
Structure Analysis ◽  
Population Genetic Structure ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Population Genetic ◽  
Mosaic Disease ◽  
Yellow Mosaic Disease

Mung bean [Vigna radiata (L.) Wilczek] is an important short-duration grain legume widely known for its nutritional, soil ameliorative, and cropping system intensification properties. This study aims at evaluating genetic diversity among mung bean genotypes and detecting genomic regions associated with various yield attributing traits and yellow mosaic disease (YMD) resistance by association mapping. A panel of 80 cultivars and advanced breeding lines was evaluated for 10 yield-related and YMD resistance traits during kharif (monsoon) and summer seasons of 2018–2019 and 2019–2020. A total of 164 genome-wide simple sequence repeat (SSR) markers were initially screened, out of which 89 were found polymorphic which generated 317 polymorphic alleles with an average of 3.56 alleles per SSR locus. The number of alleles at each locus varied from 2 to 7. The population genetic structure analysis grouped different genotypes in three major clusters and three genetically distinct subpopulations (SPs) (i.e., SP-1, SP-2, and SP-3) with one admixture subpopulation (SP-4). Both cluster and population genetic structure analysis categorized the advanced mung bean genotypes in a single group/SP and the released varieties in other groups/SPs, suggesting that the studied genotypes may have common ancestral history at some level. The population genetic structure was also in agreement with the genetic diversity analysis. The estimate of the average degree of linkage disequilibrium (LD) present at the genome level in 80 mung bean genotypes unveiled significant LD blocks. Over the four seasons, 10 marker-trait associations were observed significant for YMD and four seed yield (SY)-related traits viz., days to flowering, days to maturity, plant height, and number of pods per plant using the mixed linear model (MLM) method. These associations may be useful for marker-assisted mung bean yield improvement programs and YMD resistance.

scholarly journals Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean [Vigna radiata (L.) R. Wilczek]

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244593
Author(s):  
Uttarayan Dasgupta ◽  
Gyan Prakash Mishra ◽  
Harsh K. Dikshit ◽  
Dwijesh C. Mishra ◽  
Tejas Bosamia ◽  
...  
Keyword(s):  
Vigna Radiata ◽  
Regulatory Network ◽  
Gene Networks ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Yellow Mosaic Disease ◽  
Resistance Response ◽  
Mungbean Yellow Mosaic Virus

Yellow Mosaic Disease (YMD) in mungbean [Vigna radiata (L.) R. Wilczek] is one of the most damaging diseases in Asia. In the northern part of India, the YMD is caused by Mungbean Yellow Mosaic India Virus (MYMIV), while in southern India this is caused by Mungbean Yellow Mosaic Virus (MYMV). The molecular mechanism of YMD resistance in mungbean remains largely unknown. In this study, RNA-seq analysis was conducted between a resistant (PMR-1) and a susceptible (Pusa Vishal) mungbean genotype under infected and control conditions to understand the regulatory network operating between mungbean-YMV. Overall, 76.8 million raw reads could be generated in different treatment combinations, while mapping rate per library to the reference genome varied from 86.78% to 93.35%. The resistance to MYMIV showed a very complicated gene network, which begins with the production of general PAMPs (pathogen-associated molecular patterns), then activation of various signaling cascades like kinases, jasmonic acid (JA) and brassinosteroid (BR), and finally the expression of specific genes (like PR-proteins, virus resistance and R-gene proteins) leading to resistance response. The function of WRKY, NAC and MYB transcription factors in imparting the resistance against MYMIV could be established. The string analysis also revealed the role of proteins involved in kinase, viral movement and phytoene synthase activity in imparting YMD resistance. A set of novel stress-related EST-SSRs are also identified from the RNA-Seq data which may be used to find the linked genes/QTLs with the YMD resistance. Also, 11 defence-related transcripts could be validated through quantitative real-time PCR analysis. The identified gene networks have led to an insight about the defence mechanism operating against MYMIV infection in mungbean which will be of immense use to manage the YMD resistance in mungbean.

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

scholarly journals Current status of mosaic disease on patchouli and its control in Indonesia

Perspektif ◽  
2017 ◽  
Vol 15 (2) ◽  
pp. 87
Author(s):  
Rita Noveriza
Keyword(s):  
Early Detection ◽  
Disease Control ◽  
Mosaic Disease ◽  
Detection Methods ◽  
Current Status ◽  
Citronella Oil ◽  
North Sulawesi ◽  
Pogostemon Cablin

<p align="center">ABSTRACK</p><p>In Indonesia, the patchouli plant has been developed in twenty one Province and now there are 10 provinces that would be the area of development of patchouli with an area of 150 ha and a seed garden is focused in North Sulawesi with an area of 6 hectares. Mosaic disease on patchouli has been developing very fast, within a period of 3 years have spread to the central cultivation of patchouli in Sumatera, Java and Sulawesi. This is mainly due to the multiplication of patchouli by vegetative cutting. Therefore, the use of virus-free seed patchouli and early detection methods of patchouli seed is a major concern. In addition, the maintenance of patchouli seeds in the nursery to be free of mosaic disease and its vector is very important. Sanitation and spraying the plants with formulation of clove and citronella oil needs to be done every one or two weeks to protect the seed patchouli in nursery and every four weeks in field.</p><p>Keywords: Pogostemon cablin, Potyvirus, biopesticide, mosaic disease control.</p><p> </p><p align="center">ABSTRAK</p><p>Status terkini penyakit mosaik pada tanaman nilan dan pengendaliannya di Indonesia</p><p>Di Indonesia, tanaman nilam telah dikembangkan di duapuluh satu Provinsi dan sekarang ada 10 provinsi yang akan menjadi daerah pengembangan nilam dengan luas 150 ha dan kebun bibit difokuskan di Sulawesi Utara dengan luas 6 hektar. Penyakit mosaik dari nilam telah berkembang sangat cepat, dalam jangka waktu 3 tahun telah menyebar ke budidaya pusat nilam di Sumatera, Jawa dan Sulawesi. Hal ini terutama disebabkan oleh perbanyakan nilam dengan memotong vegetatif. Oleh karena itu, penggunaan nilam benih bebas virus dan metode deteksi dini benih nilam merupakan perhatian utama. Selain itu, pemeliharaan bibit nilam di persemaian untuk bebas dari penyakit mosaik dan vektor yang sangat penting. Sanitasi dan penyemprotan tanaman dengan formulasi cengkeh dan minyak sereh perlu dilakukan setiap satu atau dua minggu untuk melindungi benih nilam di persemaian dan setiap empat minggu di lapangan.</p>Kata kunci: Pogostemon cablin, Potyvirus, biopestisida, pengendalian penyakit mosaik.

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