Speed-Breeding System in Soybean: Integrating Off-Site Generation Advancement, Fresh Seeding, and Marker-Assisted Selection

Speed breeding by artificial control of photothermal conditions facilitates generation advancement but was limited in scale and cost. In this study, we demonstrated a cost-saving off-site summer nursery pattern, taking full advantage of shorter daylength and higher temperature with lower latitude compared to the origin of the soybean cultivars used in the study. This substantially reduced the generation cycles under totally natural conditions. Using this approach, two generations of soybean cultivars from Northeastern Spring Planting Region (NE) and Yellow-Huai-Hai Valleys Summer Planting Region (YHH) were successfully obtained in Beijing and Hainan, respectively, compared to one generation in origin. Fresh-seeding method was also used to further shorten the generation duration by 7–10 days, thereby allowing at least four generations per year. Using DNA markers to define haplotypes of maturity genes E1–E4, we proposed a model to predict the optimum adaptation region of the advanced generation lines. Taken together, we present a speed-breeding methodology combining off-site nursery, fresh-seeding method, and marker-assisted selection, aimed at accelerating soybean improvement.

Download Full-text

Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers

Theoretical and Applied Genetics ◽

10.1007/s001220051558 ◽

2000 ◽

Vol 101 (5-6) ◽

pp. 892-896 ◽

Cited By ~ 76

Author(s):

M. Ahmad

Keyword(s):

Molecular Marker ◽

Dna Markers ◽

Marker Assisted Selection ◽

Bread Quality ◽

Wheat Bread ◽

Hmw Glutenin ◽

Selection Of

Download Full-text

Selecting Soybean Cultivars for Dual Resistance to Soybean Cyst Nematode and Sudden Death Syndrome Using Two DNA Markers

Crop Science ◽

10.2135/cropsci1999.0011183x003900040005x ◽

1999 ◽

Vol 39 (4) ◽

pp. 982-987 ◽

Cited By ~ 51

Author(s):

R. R. Prabhu ◽

V. N. Njiti ◽

B. Bell‐Johnson ◽

J. E. Johnson ◽

M. E. Schmidt ◽

...

Keyword(s):

Sudden Death ◽

Dna Markers ◽

Soybean Cyst Nematode ◽

Cyst Nematode ◽

Sudden Death Syndrome ◽

Soybean Cultivars

Download Full-text

Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Southern catfish (Silurus meridionalis)

Aquaculture ◽

10.1016/j.aquaculture.2019.734783 ◽

2020 ◽

Vol 517 ◽

pp. 734783 ◽

Cited By ~ 5

Author(s):

Shuqing Zheng ◽

Xiaoshuang Wang ◽

Shuai Zhang ◽

Juan Long ◽

Wenjing Tao ◽

...

Keyword(s):

Dna Markers ◽

Marker Assisted Selection ◽

Silurus Meridionalis

Download Full-text

ANIMAL BREEDING AND GENETICS | DNA Markers and Marker-Assisted Selection

Encyclopedia of Meat Sciences ◽

10.1016/b0-12-464970-x/00002-7 ◽

2004 ◽

pp. 19-27

Author(s):

E.A. Navajas ◽

G. Simm

Keyword(s):

Dna Markers ◽

Marker Assisted Selection ◽

Animal Breeding ◽

Breeding And Genetics

Download Full-text

Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers

Plant Soil and Environment ◽

10.17221/3559-pse ◽

2011 ◽

Vol 51 (No. 2) ◽

pp. 82-86 ◽

Cited By ~ 1

Author(s):

P. Sedlák ◽

P. Vejl ◽

M. Melounová ◽

P. Křenek ◽

J. Domkářová ◽

...

Keyword(s):

Resistance Genes ◽

Dna Markers ◽

Marker Assisted Selection ◽

Potato Breeding ◽

Potato Research ◽

Plant Production ◽

Potato Chromosome ◽

Research Institutes ◽

New Varieties ◽

Developing Area

Marker assisted selection (MAS) in potato breeding is the most developing area at present time. Methods of DNA markers are developed in all top world potato research institutes and universities oriented on plant production. This paper presents results obtained from the testing of gene resources encompassing different resistance genes against Phytophthora infestans potentially exploitable for Czech potato breeding. Three different DNA markers were studied that are linked to R1 locus in the potato chromosome V operating as a resistance against some races of P. infestans. Markering capability and frequencies of these markers was evaluated with respect to their usage in practical Czech plant breeding that has vital importance in finding the tools as a prerequisite for creating new varieties efficiently.

Download Full-text

Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method

ISRN Molecular Biology ◽

10.1155/2014/828102 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Letícia A. de C. Lara ◽

João B. dos Santos ◽

Juliana S. Veloso ◽

Marcio Balestre ◽

Filipe C. Alves ◽

...

Keyword(s):

Common Bean ◽

Dna Markers ◽

Marker Assisted Selection ◽

Test Method ◽

White Mold ◽

Phenotypic Analysis ◽

Mean Values ◽

High Heritability ◽

Bayesian Shrinkage ◽

The Bayesian Approach

The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F2 population of common bean evaluated by the straw test method. The DNA from 186 F2 plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F2:4 progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F2:4 progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance—13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection.

Download Full-text

Using markers in gene introgression breeding programs.

Genetics ◽

10.1093/genetics/132.4.1199 ◽

1992 ◽

Vol 132 (4) ◽

pp. 1199-1210 ◽

Cited By ~ 7

Author(s):

F Hospital ◽

C Chevalet ◽

P Mulsant

Keyword(s):

Population Size ◽

Marker Assisted Selection ◽

Gene Introgression ◽

Selection Intensity ◽

Breeding Programs ◽

Introgression Breeding ◽

Size Number ◽

Theoretical Predictions ◽

Two Generations ◽

High Selection

Abstract We investigate the use of markers to hasten the recovery of the recipient genome during an introgression breeding program. The effects of time and intensity of selection, population size, number and position of selected markers are studied for chromosomes either carrying or not carrying the introgressed gene. We show that marker assisted selection may lead to a gain in time of about two generations, an efficiency below previous theoretical predictions. Markers are most useful when their map position is known. In the early generations, it is shown that increasing the number of markers over three per non-carrier chromosome is not efficient, that the segment surrounding the introgressed gene is better controlled by rather distant markers unless high selection intensity can be applied, and that selection on this segment first can reduce the selection intensity available for selection on non-carrier chromosomes. These results are used to propose an optimal strategy for selection on the whole genome, making the most of available material and conditions (e.g., population size and fertility, genetic map).

Download Full-text

Marker-assisted breeding of onion (A. cepa L.) maintainer line resistant to Downy mildew

VEGETABLE CROPS OF RUSSIA ◽

10.18619/2072-9146-2021-3-34-39 ◽

2021 ◽

pp. 34-39

Author(s):

Ya. T. Eidlin ◽

G. F. Monakhos ◽

S. G. Monakhos

Keyword(s):

Downy Mildew ◽

Inbred Line ◽

Marker Assisted Selection ◽

Dna Marker ◽

F1 Hybrids ◽

Marker Assisted Breeding ◽

Maintainer Line ◽

Breeding Programs ◽

Maternal Line ◽

Two Generations

Relevance. F1 hybrids breeding programs of onion are based on the use of nuclear cytoplasmic male sterility (NCMS). Breeding and seed production based on NCMS suggest a development of a female sterile maternal line, a maintainer line and a paternal component. The development of an isogenic pair of sterile line – maintainer line is one of the most labor-, time-consuming and intellectually-intensive stages of a breeding program, the implementation of which becomes more complicated with an increase in the number of traits / genes for which selection is carried out.Methods and results. This paper presents a genetic scheme for the development of a Downy mildewresistant (pathogen P. destructor) maintainer line of onion using marker-assisted selection (MAS). F2 populations were obtained from the cross of the female maintainer inbred line, Bn1- (13) (genotype – cytN msms pdpd) and male inbred line resistant to Downy mildew, No. 136 (genotype – cytT MsMs PdPd). DNA marker DMR1 were used for Downy mildew resistance gene Pd, jnurf13 – for maintainer gene ms and marker system 5`cob: orfA501 – for N, S and T cytoplasms. It was shown that the MAS application allow to develop a stable Downy mildew resistant maintainer line cytN msms PdPd in two generations.

Download Full-text