scholarly journals Isolation and Molecular Characteristics of a CIAV Isolate From Pigeons, China

2021 ◽  
Vol 8 ◽  
Author(s):  
Hongxia Shao ◽  
Jinzhi Li ◽  
Huisha Yuan ◽  
Lifei Ji ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Molecular Epidemiology ◽  
Molecular Characteristics ◽  
Chicken Infectious Anemia Virus ◽  
High Virulence ◽  
Chicken Infectious Anemia ◽  
Cross Transmission ◽  
Group A ◽  
Brazil Strain ◽  
Anemia Virus

Chicken infectious anemia virus (CIAV) mainly infects chickens and causes immunosuppression. In this study, a CIAV isolate, designated as Pigeon-CIAV-1906, was efficiently isolated from two sick pigeons by inoculating the samples into MSB1 cells. The genome of Pigeon-CIAV-1906 was amplified by PCR and analyzed. The genome size of Pigeon-CIAV-1906 was 2,298 bp with the highest homology (99.5%) to Jilin strain (JL14023) and the lowest homology (91.5%) to Brazil strain (KY024579), which phylogenetically clustered into Group A. Notably, several amino acids such as 139K and 394Q related with high virulence were found in the VP1 of Pigeon-CIAV-1906. The isolation of Pigeon-CIAV-1906 and its molecular characteristics provide evidence for the cross-transmission of CIAV from chicken to pigeon and give novel insights into the molecular epidemiology of CIAV.

scholarly journals Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 944
Author(s):  
Giulia Quaglia ◽  
Giulia Mescolini ◽  
Elena Catelli ◽  
Giacomo Berto ◽  
Filippo Muccioli ◽  
...  
Keyword(s):  
Dna Amplification ◽  
Molecular Characteristics ◽  
Distribution Map ◽  
Present Survey ◽  
Broiler Breeder ◽  
Non Invasive ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus ◽  
Backyard Chicken

Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

SEROLOGICAL STUDIES ON CHICKEN INFECTIOUS ANEMIA VIRUS IN GHARBEIA PROVINCE

2008 ◽  
Vol 6 (2) ◽  
pp. 196-208
Author(s):  
Moshira, A. El-abasy ◽  
Gamal, R. Hassab El-nabby ◽  
Ahmed, S. Hegasy ◽  
Salwa, M. Helmy
Keyword(s):  
Chicken Infectious Anemia Virus ◽  
Serological Studies ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

scholarly journals Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding δEF1

2008 ◽  
Vol 89 (12) ◽  
pp. 2998-3003 ◽  
Author(s):  
Myrna M. Miller ◽  
Keith W. Jarosinski ◽  
Karel A. Schat
Keyword(s):  
Fluorescent Protein ◽  
Transcription Start ◽  
Upstream Promoter ◽  
Chicken Infectious Anemia Virus ◽  
E Box ◽  
Promoter Construct ◽  
Chicken Infectious Anemia ◽  
Lmh Cells ◽  
Green Fluorescent ◽  
Anemia Virus

Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (δEF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and δEF1.

Chicken Infectious Anemia Virus Infection in Israeli Commercial Flocks: Virus Amplification, Clinical Signs, Performance, and Antibody Status

10.1637/7072 ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 108-118 ◽  
Author(s):  
I. Davidson ◽  
M. Kedem ◽  
H. Borochovitz ◽  
N. Kass ◽  
G. Ayali ◽  
...  
Keyword(s):  
Virus Infection ◽  
Clinical Signs ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

Immune Complex Vaccines for Chicken Infectious Anemia Virus

2011 ◽  
Vol 6 (1) ◽  
pp. e21-e22
Author(s):  
Karel A Schat ◽  
Nelson Rodrigo da Silva Martins ◽  
Priscilla H O'Connell ◽  
Michael S Piepenbrink
Keyword(s):  
Immune Complex ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

scholarly journals Synergetic pathogenicity of Newcastle disease vaccines LaSota strain and contaminated chicken infectious anemia virus

2019 ◽  
Vol 98 (5) ◽  
pp. 1985-1992 ◽  
Author(s):  
Qi Su ◽  
Tuanjie Wang ◽  
Fanfeng Meng ◽  
Zhizhong Cui ◽  
Shuang Chang ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus
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