Dietary Concentrate Supplementation Alters Serum Metabolic Profiles Related to Energy and Amino Acid Metabolism in Grazing Simmental Heifers

Supplementation plays a vital role in the growth performance of grazing heifers. We investigated the effects of maize-based concentrate supplementation on the serum metabolome in grazing heifers. Twenty-four 7-month-old heifers (211.65 ± 4.25 kg BW) were randomly divided into a supplement (SUP) group and a control (CON) group. The results indicated that concentrate supplementation increased the final body weight (BW) of grazing heifers, and the average daily gain (ADG) was 61.5% (P = 0.011) higher in the SUP group than in the CON group. Serum concentrations of total protein (TP), triglyceride (TG), and leptin were higher in the SUP group than in the CON group (p < 0.05). Supplementation increased serum metabolites and amino acids and markedly altered glucose, lipid, and protein metabolism, which contributed to the heifer growth. Furthermore, by multivariate analysis, 45 serum metabolites were identified as significantly different between the two groups. Enrichment analysis revealed that arginine biosynthesis and tryptophan metabolism as well as glycerophospholipid metabolism were significantly enriched between the two groups. We concluded that the growth potential of heifers could be improved by maize-based concentrate supplementation, and the main biological pathways affected were those related to energy and amino acid metabolism.

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Comprehensive transcriptome and metabolome profiling reveal metabolic mechanisms of Nitraria sibirica Pall. to salt stress

Scientific Reports ◽

10.1038/s41598-021-92317-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Huanyong Li ◽

Xiaoqian Tang ◽

Xiuyan Yang ◽

Huaxin Zhang

Keyword(s):

Salt Stress ◽

Amino Acid ◽

Salt Tolerance ◽

Metabolic Pathways ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Sulfur Metabolism ◽

Enrichment Analysis ◽

Extracellular Region ◽

Nitraria Sibirica

AbstractNitraria sibirica Pall., a typical halophyte that can survive under extreme drought conditions and in saline-alkali environments, exhibits strong salt tolerance and environmental adaptability. Understanding the mechanism of molecular and physiological metabolic response to salt stress of plant will better promote the cultivation and use of halophytes. To explore the mechanism of molecular and physiological metabolic of N. sibirica response to salt stress, two-month-old seedlings were treated with 0, 100, and 400 mM NaCl. The results showed that the differentially expressed genes between 100 and 400 mmol L−1 NaCl and unsalted treatment showed significant enrichment in GO terms such as binding, cell wall, extemal encapsulating structure, extracellular region and nucleotide binding. KEGG enrichment analysis found that NaCl treatment had a significant effect on the metabolic pathways in N. sibirica leaves, which mainly including plant-pathogen interaction, amino acid metabolism of the beta alanine, arginine, proline and glycine metabolism, carbon metabolism of glycolysis, gluconeogenesis, galactose, starch and sucrose metabolism, plant hormone signal transduction and spliceosome. Metabolomics analysis found that the differential metabolites between the unsalted treatment and the NaCl treatment are mainly amino acids (proline, aspartic acid, methionine, etc.), organic acids (oxaloacetic acid, fumaric acid, nicotinic acid, etc.) and polyhydric alcohols (inositol, ribitol, etc.), etc. KEGG annotation and enrichment analysis showed that 100 mmol L−1 NaCl treatment had a greater effect on the sulfur metabolism, cysteine and methionine metabolism in N. sibirica leaves, while various amino acid metabolism, TCA cycle, photosynthetic carbon fixation and sulfur metabolism and other metabolic pathways have been significantly affected by 400 mmol L−1 NaCl treatment. Correlation analysis of differential genes in transcriptome and differential metabolites in metabolome have found that the genes of AMY2, BAM1, GPAT3, ASP1, CML38 and RPL4 and the metabolites of L-cysteine, proline, 4-aminobutyric acid and oxaloacetate played an important role in N. sibirica salt tolerance control. This is a further improvement of the salt tolerance mechanism of N. sibirica, and it will provide a theoretical basis and technical support for treatment of saline-alkali soil and the cultivation of halophytes.

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PSIII-23 Characterization of oxidized oil-elicited disruption of amino acid metabolism in nursery pigs through metabolomic analysis

Journal of Animal Science ◽

10.1093/jas/skz122.312 ◽

2019 ◽

Vol 97 (Supplement_2) ◽

pp. 177-177

Author(s):

Yue Guo ◽

Andrea Hanson ◽

Lei Wang ◽

Brian Kerr ◽

Pedro Urriola ◽

...

Keyword(s):

Amino Acid ◽

Growth Performance ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Corn Oil ◽

Phase 1 ◽

Average Daily Gain ◽

Metabolomics Data ◽

Nursery Pigs ◽

Metabolomics Analysis

Abstract Feeding oxidized lipids compromises growth performance of pigs, but the metabolic events contributing to this adverse effect are not well defined. In this study, oxidized corn oil (OCO) was prepared by heating control corn oil (CCO) at 185 oC for 12 h. Weanling pigs (initial BW = 6.3 ± 1.4 kg) were fed 4 isocaloric diets containing 9% CCO, 6% CCO + 3% OCO, 3% CCO + 6% OCO, and 9% OCO, respectively. Pigs were provided ad libitum access to experimental diets in 3 phases (phase 1 = 4 d, phase 2 = 10 d and phase 3 = 21 d) for 35 d. Pig body weight (BW) and feed disappearance were determined at the d 0, 4, 14 and 35 to calculate average daily gain (ADG), average daily feed intake (ADFI) and gain to feed (G:F) ratio. Serum and liver samples collected on d 35 of feeding were analyzed by the liquid chromatography-mass spectrometry (LC-MS)-based metabolomics analysis. Growth performance data were analyzed using the MIXED procedure of SAS and metabolomics data were analyzed by two-tailed student’s t test for comparison between different doses of OCO and CCO treatments. The result showed that dietary OCO decreased G:F ratio (P < 0.05) dose-dependently, but did not ADFI. Metabolomics analysis showed that OCO fed pigs decreased the levels of serum alanine (P < 0.01), tryptophan (P < 0.05), carnosine (P < 0.01), and glutamic acid (P < 0.05), while the levels of threonine (P < 0.05) was increased compared to CCO. Moreover, consuming OCO decreased the hepatic metabolites from threonine catabolism pathways, including α-ketobutyrate (P < 0.01), α-amino-butyrate (P < 0.05), and propionic acid (P < 0.05), compared to CCO treatment. In addition, OCO increased hepatic NAD level by activating tryptophan-NAD+ metabolic pathway. Overall, OCO selectively modulated amino acid metabolism in nursery pigs, which may further affect growth performance.

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Comparative Proteomic Analysis of Leaves at Different Ages in Allotriploid Populus

Forests ◽

10.3390/f11111154 ◽

2020 ◽

Vol 11 (11) ◽

pp. 1154

Author(s):

Jiang Li ◽

Yi Wang ◽

Hairong Wei ◽

Xiangyang Kang

Keyword(s):

Amino Acid ◽

Carbon Metabolism ◽

Proteomic Analysis ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Leaf Age ◽

Enrichment Analysis ◽

C1 Metabolism ◽

Comparative Proteomic Analysis ◽

N Metabolism

Triploid poplar trees have been shown to have a number of growth advantages, especially much bigger leaves that contribute greatly to the increased biomass. In this study, we focused on the relationships between leaf age and leaf metabolism in triploids. We performed comparative proteomic analysis of the 5th (FDR5), 10th (FDR10), and 25th (FDR25) leaves from the apical meristems in allotriploids originated from first-division restitution (FDR). A total of 1970, 1916, and 1850 proteins were identified in the FDR5, FDR10, and FDR25, respectively. Principle component analysis (PCA) and differentially accumulated protein (DAP) analysis showed that FDR10 and FDR25 displayed higher similarities of protein accumulation patterns as compared to FDR5. MapMan enrichment analysis showed that several primary metabolic pathways or processes were significantly enriched in the DAPs. For example, photosynthesis, major CHO metabolism, glycolysis, N metabolism, redox, C1-metabolism, DNA, and protein turnover were significantly altered in both FDR10 and FDR25 compared with FDR5. In addition, amino acid metabolism and gluconeogenesis/glyoxylate cycle also underwent significant changes in FDR25 compared with FDR5. However, only amino acid metabolism was significantly enriched in the DAPs between FDR25 and FDR10. Further, DAP accumulation pattern analysis implied that FDR5, FDR10, and FDR25 were placed in the young, mature, and primary senescence stages of leaves. The most DAPs involved in the light reaction, photorespiration, Calvin cycle, starch and sucrose metabolism, pentose phosphate pathway (OPP), tricarboxylic acid (TCA) cycle, N metabolism, and C1-metabolism displayed higher accumulation in both FDR10 and FDR25 compared to FDR5. However, the most DAPs that are involved in cell wall and lipid metabolism, tetrapyrrole synthesis, nucleotide metabolism exhibited lower accumulation in both FDR10 and FDR25. Almost all DAPs between FDR-10 and FDR-25 showed a dramatic decrease in FDR25. KEGG enrichment analysis showed that carbon metabolism was altered significantly at different leaf ages. DAPs that are involved in carbon metabolism were predicted as different points in protein–protein interaction (PPI) networks from the STRING database. Finally, inconsistent transcript and protein abundance was found for DAPs, indicating the presence of posttranscriptional regulation during leaf-age progression process.

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