scholarly journals The In-Silico Development of DNA Markers for Breeding of Spring Barley Varieties That Are Resistant to Spot Blotch in Russia

Agriculture ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 505
Author(s):  
Irina V. Rozanova ◽  
Nina M. Lashina ◽  
Vadim M. Efimov ◽  
Olga S. Afanasenko ◽  
Elena K. Khlestkina
Keyword(s):  
Dna Markers ◽  
Root Rot ◽  
Spring Barley ◽  
Significant Snps ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Yield Losses ◽  
Barley Cultivars ◽  
Northwestern Region ◽  
The Impact

The fungal pathogen Cochliobolus sativus Drechs. Ex Dastur, anamorph Bipolaris sorokiniana (Sacc.) Shoemaker is one of the most common barley pathogens worldwide and causes spot blotch and root rot in barley. Spot blotch is considered to be the major biotic stress hampering the commercial production of barley. During high disease severity, which occurs in the northwestern region of Russia once every three to four years, yield losses for barley may reach 40%. An increase in common root rot severity results in yield losses that can reach 80%. The goal of the current study was to identify significant markers that can be employed as diagnostic DNA markers to breed C. sativus pathogen-resistant varieties of barley. In 94 spring barley cultivars and lines, the resistance of seedlings and adult plants to the impact of C. sativus on their leaves and roots was investigated. Five genomic regions associated with resistance to Spot blotch were identified (on chromosome 1H (50–61.2 cM), 2H (68.7–69.68 cM), 3H (18.72–26.18 cM), 7H (7.52–15.44 cM)). No significant loci were determined to be associated with root rot. According to obtained data, 11 significant SNPs were converted into KASP markers and 6 markers located on chromosome 3H were determined to possess good accuracy and the potential to be employed in marker-assisted selection.

scholarly journals Reaction of spring barley cultivars grown in Finland to soil-borne infection by Bipolaris sorokiniana and to its toxic metabolites

1985 ◽  
Vol 57 (2) ◽  
pp. 85-96
Author(s):  
Aarne Kurppa
Keyword(s):  
Root Rot ◽  
Spring Barley ◽  
Severe Disease ◽  
Bipolaris Sorokiniana ◽  
Toxin Production ◽  
Yield Losses ◽  
Disease Symptoms ◽  
Barley Cultivars ◽  
Toxic Metabolites ◽  
The Mean

Soil-borne infection of Bipolaris sorokiniana caused foot and root rot in all spring barley cultivars studied. Significant differences in susceptibility of the cultivars and pathogenicity of the fungus isolates were found. Primary symptoms caused by the fungus were seedling blight, later foot and root rot. Yield losses caused by the fungus varied from 3 % to 33 % the mean being c. 15 %. Yield losses could occur without severe disease symptoms. Toxic metabolites produced by the fungus induced visible foot and root symptoms in all cultivars tested and caused lesions in the leaves of some cultivars. Variability in toxin production of fungus isolates as well as the reaction of a cultivar to toxins was demonstrated. The cultivars most susceptible to soil-borne infection by the fungus also showed the most severe symptoms when exposed to toxic metabolites of the fungus.

scholarly journals The response of some spring barley cultivars grown in Finland to air-borne secondary infection by Bipolaris sorokiniana

1985 ◽  
Vol 57 (2) ◽  
pp. 97-105
Author(s):  
Aarne Kurppa
Keyword(s):  
Secondary Infection ◽  
Spring Barley ◽  
Bipolaris Sorokiniana ◽  
Yield Losses ◽  
Foliar Diseases ◽  
Infection Incidence ◽  
Barley Cultivars ◽  
Internal Cell ◽  
High Infection ◽  
The Mean

Air-borne secondary inoculum of Bipolaris sorokiniana caused severe foliar diseases and yield losses in all 12 spring barley cultivars tested in greenhouses or in the field. For secondary infection to occur a high relative humidity was necessary. Yield losses due to foliar diseases reached a maximum of 43.4 % in greenhouse experiments and 27.8 % in the field. The mean losses were 20.3 % and 12.3%, respectively. Early infection at the time of heading or shortly after it resulted in higher yield losses than did later infection, although the symptom expression was opposite. Spore inoculation or natural secondary infection by the spores from a diseased crop after heading always resulted in a high infection incidence in the grain. Infection incidence as well as fungal invasion of the internal cell leyers of the grains varied significantly among barley cultivars. The most susceptible of those tested were cvs. Teemu, Paavo and Pomo, while the most resistant were Ingrid, Otra and Pirkka.

scholarly journals Molecular Mapping of Loci Conferring Susceptibility to Spot Blotch and Resistance to Powdery Mildew in Barley Using the Sequencing-Based Genotyping Approach

2020 ◽  
Vol 110 (2) ◽  
pp. 440-446 ◽  
Author(s):  
Yueqiang Leng ◽  
Mingxia Zhao ◽  
Jason Fiedler ◽  
Antonín Dreiseitl ◽  
Shiaoman Chao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Molecular Mapping ◽  
Recombinant Inbred Lines ◽  
Major Gene ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Blumeria Graminis ◽  
Seedling Stage ◽  
Barley Cultivars ◽  
Trait Locus

Spot blotch (SB) caused by Bipolaris sorokiniana and powdery mildew (PM) caused by Blumeria graminis f. sp. hordei are two important diseases of barley. To map genetic loci controlling susceptibility and resistance to these diseases, a mapping population consisting of 138 recombinant inbred lines (RILs) was developed from the cross between Bowman and ND5883. A genetic map was constructed for the population with 852 unique single nucleotide polymorphism markers generated by sequencing-based genotyping. Bowman and ND5883 showed distinct infection responses at the seedling stage to two isolates (ND90Pr and ND85F) of Bipolaris sorokiniana and one isolate (Race I) of Blumeria graminis f. sp. hordei. Genetic analysis of the RILs revealed that one major gene (Scs6) controls susceptibility to Bipolaris sorokiniana isolate ND90Pr, and another major gene (Mla8) confers resistance to Blumeria graminis f. sp. hordei isolate Race I, respectively. Scs6 was mapped on chromosome 1H of Bowman, as previously reported. Mla8 was also mapped to the short arm of 1H, which was tightly linked but not allelic to the Rcs6/Scs6 locus. Quantitative trait locus (QTL) analysis identified two QTLs, QSbs-1H-P1 and QSbs-7H-P1, responsible for susceptibility to spot blotch caused by Bipolaris sorokiniana isolate ND85F in ND5883, which are located on chromosome 1H and 7H, respectively. QSbs-7H-P1 was mapped to the same region as Rcs5, whereas QSbs-1H-P1 may represent a novel allele conferring seedling stage susceptibility to isolate ND85F. Identification and molecular mapping of the loci for SB susceptibility and PM resistance will facilitate development of barley cultivars with resistance to the diseases.

Structure of Polish isolates ofBipolaris sorokinianaand effect of different pathotypes on spot blotch severity of selected spring barley cultivars

2019 ◽  
Vol 47 (2) ◽  
pp. 314-323
Author(s):  
M. Cegiełko ◽  
M. Wit ◽  
I. Kiecana ◽  
W. Wakuliński ◽  
E. Mielniczuk
Keyword(s):  
Spring Barley ◽  
Spot Blotch ◽  
Barley Cultivars

ETUDE DE LA TOLERANCE DE CULTIVARS D’ORGE A LA TACHE HELMINTHOSPORIENNE

1987 ◽  
Vol 67 (1) ◽  
pp. 153-157 ◽  
Author(s):  
D. DOSTALER ◽  
G. J. PELLETIER ◽  
L. COUTURE
Keyword(s):  
Hordeum Vulgare ◽  
Grain Yield ◽  
Seed Weight ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Grain Weight ◽  
Disease Development ◽  
Barley Cultivars ◽  
Moderate Disease ◽  
Key Words Barley

This study was conducted to assess tolerance of barley (Hordeum vulgare) cultivars to spot blotch. The barley cultivars Parkland, Bonanza and Laurier inoculated with spores of Bipolaris sorokiniana developed approximately the same level of spot blotch symptoms in the field. Natural disease development was minimal in plots treated with mancozeb; it was intermediate in uninoculated and untreated plots. Reduction of grain yield and grain weight differed with each cultivar. Disease had the most depressing effects on Parkland. Disease severity was highest on Laurier which suffered the least decrease in grain yield and grain weight compared to the checks. Yield and seed weight of Bonanza were not affected by low or moderate disease levels (checks) but were reduced when symptoms were severe (inoculations).Key words: Barley, cultivars, spot blotch, Bipolaris sorokiniana, tolerance, mancozeb

scholarly journals Efficacy of Bacillus SPP. strains against barley diseases caused by Bipolaris Sorokiniana on cultivars of different resistance

2020 ◽  
Vol 11 (4) ◽  
Author(s):  
L. O. Kriuchkova ◽  
◽  
T. I. Patyka ◽  
Keyword(s):  
Root Rot ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Growth Media ◽  
Common Root ◽  
Common Root Rot ◽  
Medium Resistance ◽  
Bacillus Spp ◽  
The Common ◽  
Bacillus Strains

The effect of Bacillus strains, B. subtilis 16 and B. pumilus 11, on two barley diseases caused by Bipolaris sorokiniana (Sacc.) Shoemaker has been studied. It was found that the activity depends on both the resistance of the cultivar against disease and the bacterial strain. The severity of spot blotch of barley decreased significantly when the cell suspension of B. subtilis strain16 was sprayed on the leaves of disease-susceptible cultivars Nashсhadok, but failed to inhibit the disease on the leaves of resistant cultivars. Applying the cell suspensions into plant growth media significantly inhibited the common root rot on susceptible cultivar Syaivo, which, however, was not accompanied by an increase in the biomass of seedlings. A significant increase in the weight of shoots and roots of seedlings was observed on cultivars with medium resistance to the disease: Sebastian and Antey.

scholarly journals Genetics of wheat somaclones resistance to Bipolaris sorokiniana Shoem.

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. 186-188
Author(s):  
L.G. Tyryshkin ◽  
N.A. Tyryshkina-Shishelova
Keyword(s):  
Root Rot ◽  
Initial Level ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Genetics Of Resistance ◽  
Common Root ◽  
Common Root Rot ◽  
Brown Leaf Spot ◽  
Brown Leaf ◽  
Durability Of Resistance

Genetics of resistance to common root rot and dark brown leaf spot blotch (both caused by Bipolaris sorokiniana Shoem.)<br />was studied in wheat somaclonal lines, obtained in calluses culture of samples 181-5 and Vera. Four different approaches<br />were used: linear analysis of resistance in generations of segregating somaclonal lines, hybridological analysis, study<br />of resistance components, study of possible durability of resistance. Results showed, that resistance to both diseases is<br />likely controlled by polygenic systems with additive actions of minor genes. Different lines possess non-identical genetic<br />systems for resistance. Several lines kept their initial level of resistance to spot blotch after 5 cycles of the pathogen<br />artificial population reproduction.

scholarly journals Pyramiding of scald resistance genes in four spring barley MAGIC populations

2021 ◽  
Author(s):  
Juho Hautsalo ◽  
Fluturë Novakazi ◽  
Marja Jalli ◽  
Magnus Göransson ◽  
Outi Manninen ◽  
...  
Keyword(s):  
Association Studies ◽  
Spring Barley ◽  
Field Trials ◽  
Snp Markers ◽  
Genome Wide Association Studies ◽  
Yield Losses ◽  
Barley Cultivars ◽  
Genome Wide ◽  
Barley Leaf ◽  
Advanced Generation

AbstractGenome-Wide Association Studies (GWAS) of four Multi-parent Advanced Generation Inter-Cross (MAGIC) populations identified nine regions on chromosomes 1H, 3H, 4H, 5H, 6H and 7H associated with resistance against barley scald disease. Three of these regions are putatively novel resistance Quantitative Trait Loci (QTL). Barley scald is caused by Rhynchosporium commune, one of the most important barley leaf diseases that are prevalent in most barley-growing regions. Up to 40% yield losses can occur in susceptible barley cultivars. Four MAGIC populations were generated in a Nordic Public–Private Pre-breeding of spring barley project (PPP Barley) to introduce resistance to several important diseases. Here, these MAGIC populations consisting of six to eight founders each were tested for scald resistance in field trials in Finland and Iceland. Eight different model covariate combinations were compared for GWAS studies, and the models that deviated the least from the expected p-values were selected. For all QTL, candidate genes were identified that are predicted to be involved in pathogen defence. The MAGIC progenies contained new haplotypes of significant SNP-markers with high resistance levels. The lines with successfully pyramided resistance against scald and mildew and the significant markers are now distributed among Nordic plant breeders and will benefit development of disease-resistant cultivars.

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