scholarly journals Evaluation of Behavioral Aspects after Intradermal and Intramuscular Vaccine Application in Suckling Piglets

Agriculture ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 637
Author(s):  
Manuel Göller ◽  
Nicole Kemper ◽  
Michaela Fels
Keyword(s):  
Social Contact ◽  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Behavior Changes ◽  
Intradermal Vaccination ◽  
Behavioral Observations ◽  
Behavioral Aspects ◽  
Suckling Piglets

The aim of this study was to analyse the behavioral aspects of suckling piglets after an intradermal vaccination method in comparison to an intramuscular vaccination applied on the seventh day of life. Possible effects on piglet welfare should be evaluated. Under field conditions, 135 suckling piglets from 12 litters were vaccinated against Mycoplasma hyopneumoniae—64 of those intradermally and 71 intramuscularly, from six litters each. For behavioral analyses, videos were recorded per pen, starting three days before the vaccination and ending three days after the vaccination. In the video analyses, the observation periods 6.00–10.00, 13.00–17.00, and 19.00–21.00 were analysed via scan sampling for the behaviors lying, standing, walking, suckling, and social contact. In the behavioral observations, in all piglets, the most frequent behavior was lying, followed by suckling at the sow’s teats. After vaccination, less lying behavior and more suckling behavior were assessed in intradermally vaccinated piglets compared to intramuscularly vaccinated piglets, which indicates that the piglets were not impaired by stress following vaccination. The results of this study showed that intradermal needle-free vaccination has clear advantages, as it caused fewer vaccination-associated behavior changes in suckling piglets than the intramuscular vaccination method with a needle.

Detection of Mycoplasma hyopneumoniae by Air Sampling with a Nested PCR Assay

1998 ◽  
Vol 64 (2) ◽  
pp. 543-548 ◽  
Author(s):  
Katharina D. C. Stärk ◽  
Jacques Nicolet ◽  
Joachim Frey
Keyword(s):  
Nested Pcr ◽  
Gene Segment ◽  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Pcr Analysis ◽  
Direct Extraction ◽  
Pcr Assay ◽  
Sampling System ◽  
Respiratory Problems ◽  
One Step

ABSTRACT This article describes the first successful detection of airborneMycoplasma hyopneumoniae under experimental and field conditions with a new nested PCR assay. Air was sampled with polyethersulfone membranes (pore size, 0.2 μm) mounted in filter holders. Filters were processed by dissolution and direct extraction of DNA for PCR analysis. For the PCR, two nested pairs of oligonucleotide primers were designed by using an M. hyopneumoniae-specific DNA sequence of a repeated gene segment. A nested PCR assay was developed and used to analyze samples collected in eight pig houses where respiratory problems had been common. Air was also sampled from a mycoplasma-free herd. The nested PCR was highly specific and 104 times as sensitive as a one-step PCR. Under field conditions, the sampling system was able to detect airborne M. hyopneumoniae on 80% of farms where acute respiratory disease was present. No airborne M. hyopneumoniae was detected on infected farms without acute cases. The chance of successful detection was increased if air was sampled at several locations within a room and at a lower air humidity.

The Mycoplasma hyopneumoniae recombinant heat shock protein P42 induces an immune response in pigs under field conditions

2014 ◽  
Vol 37 (4) ◽  
pp. 229-236 ◽  
Author(s):  
Sérgio Jorge ◽  
Natasha Rodrigues de Oliveira ◽  
Silvana Beutinger Marchioro ◽  
Andressa Fisch ◽  
Charles Klazer Gomes ◽  
...  
Keyword(s):  
Immune Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Mycoplasma Hyopneumoniae ◽  
Field Conditions

scholarly journals Evaluation of Mycoplasma hyopneumoniae Inactivated Vaccine in Pigs under Field Conditions.

1999 ◽  
Vol 61 (10) ◽  
pp. 1131-1135 ◽  
Author(s):  
Munenori OKADA ◽  
Tetsuya SAKANO ◽  
Kazuhiro SENNA ◽  
Takashi MARUYAMA ◽  
Junichi MUROFUSHI ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Inactivated Vaccine

scholarly journals Field Trial Assessment of Two Commercial Vaccines Against Mycoplasma Hyopneumoniae

2020 ◽  
Author(s):  
Marcial Marcos ◽  
Javier Rabanal ◽  
David del Pico ◽  
Jesús Bollo ◽  
Alfredo Romero ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Lung Lesion ◽  
Control Group ◽  
Intradermal Vaccination ◽  
Enzootic Pneumonia ◽  
Routes Of Administration ◽  
Average Value ◽  
Group I ◽  
Group Reduction ◽  
Unvaccinated Control

Abstract Background: Mycoplasma hyopneumoniae (M.hyo) is one of the most prevalent pathogens causing respiratory disorders in swine populations as well as primary agent of porcine respiratory disease complex (PRDC). The aim of this trial is to compare the efficacy of two commercial vaccines under field conditions, applied via different routes of administration, as a tool to control disease.Results: 3 groups each consisting of 440 animals, were included in this longitudinal study; one group vaccinated against M.hyo with an intradermal vaccine (group I), a second group vaccinated with an intramuscular vaccine 2940 strain (group H) and finally a third group, not vaccinated as a control (group N). All animals were individually weighed at weaning; upon entry into the fattening unit and just prior to movement to the slaughterhouse. Pigs from each group were periodically blood sampled to evaluate the immunological status against other pathogens involved in PRDC. Tracheobronchial swabs were collected from 30 pigs per treatment group during the final weighing. Slaughterhouse lung lesion evaluations were developed to assess levels of enzootic pneumonia-like lesions (EP) and pleuritis (P).Statistical evaluation of all test parameters indicated a better performance of the intradermal vaccinated group (group I):· in terms of weight (kg) just prior to movement to the slaughterhouse, (I= 97.45; H = 93.44; N = 93.05).· The number of positive tracheobronchial swabs, as well as M.hyo load detected individually (I = 36.7% positive samples with log10 Ct average value 1.48; H = 100% with log10 Ct 1.40; and N = 96.7% with log10 Ct 1.40),· EP prevalence (I = 35.1%, H = 47.0%, N = 50.5%) and· Pleurisy prevalence (I = 22.4%, H = 29.7%, N = 39.3%).In terms of the involvement of other PRDC pathogens, the serological study could lead us to think that the agents studied were present at same level in the all 3 vaccination groups.Conclusion: In this study, intradermal vaccination provided better performance than unvaccinated control group and intramuscular vaccine, in terms of weight increase (versus intramuscular group); reduction of enzootic pneumonia and pleurisy lesions, and tracheobronchial M.hyo prevalence at the end of the finishing period.

Immune B cell responsiveness to single-dose intradermal vaccination against Mycoplasma hyopneumoniae

2021 ◽  
Author(s):  
Paolo Martelli ◽  
Roberta Saleri ◽  
Melania Andrani ◽  
Valeria Cavalli ◽  
Elena De Angelis ◽  
...  
Keyword(s):  
Single Dose ◽  
B Cell ◽  
Mycoplasma Hyopneumoniae ◽  
Intradermal Vaccination

scholarly journals Mycoplasma hyopneumoniae vaccination at or shortly before weaning under field conditions: a randomised efficacy trial

2017 ◽  
Vol 181 (1) ◽  
pp. 19-19 ◽  
Author(s):  
I. Arsenakis ◽  
A. Michiels ◽  
R. Del Pozo Sacristán ◽  
F. Boyen ◽  
F. Haesebrouck ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Field Conditions ◽  
Efficacy Trial

scholarly journals Performance of Commercial Mycoplasma hyopneumoniae Serum Enzyme-Linked Immunosorbent Assays under Experimental and Field Conditions

2020 ◽  
Vol 58 (12) ◽  
Author(s):  
Ana Paula S. Poeta Silva ◽  
Ronaldo L. Magtoto ◽  
Henrique M. Souza Almeida ◽  
Aric McDaniel ◽  
Precy D. Magtoto ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Mycoplasma Hyopneumoniae ◽  
Error Rates ◽  
False Positives ◽  
Field Conditions ◽  
Misclassification Error ◽  
Antibody Detection ◽  
Serum Samples ◽  
Content Type ◽  
Immunosorbent Assays

ABSTRACT Mycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Weekly serum and daily oral fluid samples were collected through 56 days postinoculation (dpi). The true status of pigs was established by PCR testing on oral fluids samples over the course of the observation period. Analysis of ELISA performance at various cutoffs found that the manufacturers’ recommended cutoffs were diagnostically specific, i.e., produced no false positives, with the exceptions of 2 ELISAs. An analysis based on overall misclassification error rates found that 4 ELISAs performed similarly, although one assay produced more false positives. In study 2, the 3 best-performing ELISAs from study 1 were compared using serum samples generated under field conditions. Ten 8-week-old pigs were intratracheally inoculated with M. hyopneumoniae. Matched serum and tracheal samples (to establish the true pig M. hyopneumoniae status) were collected at 7- to 14-day intervals through 98 dpi. Analyses of sensitivity and specificity showed similar performance among these 3 ELISAs. Overall, this study provides an assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance.

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