scholarly journals Screening of the Candidate DNA Barcodes for Three Important Amorphophallus Species Identification

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1366
Author(s):  
Chufeng Zhao ◽  
Xuan She ◽  
Erxi Liu ◽  
Nunung Harijati ◽  
Teng Cheng ◽  
...  

Amorphophallus is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. Amorphophallus has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to Amorphophallus and closely related species. This study used six barcodes, namely ITS2, matK, rcbL, nad1, trnH-psbA, and trnL-trnF, to evaluate their identification ability for three important Amorphophallus species, including A. konjac, A. albus, and A. muelleri. We recommend that trnH-psbA can be applied to the Amorphophallus trade, quickly identify the purity of A. konjac and A. albus and distinguish A. muelleri from its related species for A. konjac and A. albus genetic improvement.

Aquaculture ◽  
2010 ◽  
Vol 308 ◽  
pp. S47-S50 ◽  
Author(s):  
Kednapat Sriphairoj ◽  
Sirawut Klinbu-nga ◽  
Wongpathom Kamonrat ◽  
Uthairat Na-Nakorn

Author(s):  
Antoni Lombarte ◽  
Marta M. Rufino ◽  
Pilar Sánchez

The statoliths of 14 species (193 right statoliths from subadult to adult individuals), belonging to five Cephalopoda families (Sepiidae, Loliginidae, Enoploteuthidae, Ommastrephidae and Octopodidae) were analysed using morphometric methods based on landmarks (geometric morphometry). The aim of the current study is to determine the discriminating power of statolith shape analysis in species identification of Mediterranean cephalopods. Discriminant analyses of the partial warps were able to fully identify (100% discrimination) the species of all families, except Octopodidae which showed some misclassification (correctly classified about 68–90%). These results were also shown by relative warp analysis. Octopodidae statoliths were studied for the first time using geometric landmark-based methods. Greatest differences in statolith shape between Octopodidae species, were in the area that unites the statolith dome with the flat wing. Landmark analysis applied to statoliths can be a useful taxonomic tool in the identification of closely related species.


2017 ◽  
Vol 29 (7) ◽  
pp. 1121-1127
Author(s):  
Misbah Ullah ◽  
Yanling Dong ◽  
Pingping Qiao ◽  
Yalin Zhang ◽  
Zhaofu Yang

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0160611 ◽  
Author(s):  
Fan-Hong Wang ◽  
Jin-Mei Lu ◽  
Jun Wen ◽  
Atsushi Ebihara ◽  
De-Zhu Li

2014 ◽  
Vol 15 (2) ◽  
pp. 337-348 ◽  
Author(s):  
Juan Chen ◽  
Jietang Zhao ◽  
David L. Erickson ◽  
Nianhe Xia ◽  
W. John Kress

Zootaxa ◽  
2017 ◽  
Vol 4365 (3) ◽  
pp. 311
Author(s):  
REZA GHADERI ◽  
LEILA KASHI ◽  
HOSSEIN MIRBABAEI KARANI ◽  
AKBAR KAREGAR

A new and four known species of the genus Diphtherophora—D. geraerti sp. n., D. caudata, D. obesa, D. perplexans and D. tenera—were collected and identified from different plants and localities in Iran. Morphological and morphometric characters of Diphtherophora geraerti sp. n. are compared with those of closely-related species, including D. siddiqii, D. brevicollis, D. lata, D. obesa, D. citri, D. vasilevi and D. vitoshae. Females of the new species have a head continuous with the body contour, spear 11–14 µm in length, rod-shaped spermatozoa and conical tail with bluntly rounded to hemispherical terminus. Males have two ventromedian neck papillae located 22–25 and 52–60 µm from the anterior end, and two ventromedian supplements at 11–13 and 75–80 µm anterior from cloacal aperture, respectively. All Diphtherophora species recovered as part of the present study are new records for the Iranian nematode fauna. Finally, a diagnostic compendium is given, to assist with species identification in the genus. 


Zootaxa ◽  
2021 ◽  
Vol 4941 (3) ◽  
pp. 301-337
Author(s):  
PETER HUEMER ◽  
ERIK J. VAN NIEUKERKEN

Seventy-three species of Lepidoptera described from France since 2000, particularly by Jacques Nel and Thierry Varenne, are re-assessed from largely unpublished molecular data. We tried to obtain DNA barcode sequences from 62 holotypes, supplemented by paratypes of eight species and on one case by non-type material, whereas one previously synonymized species was not sequenced. Altogether we obtained 78 DNA barcode sequences for 65 nominal taxa while sequencing failed for six holotypes. An integrative analysis from molecular data and morphology supports the validity of the majority of species but also resulted in the re-assessment of several taxa. The following 13 new synonymies are established: Stigmella cyrneorolandi Nel & Varenne, 2013 syn. nov. of Stigmella rolandi van Nieukerken, 1990; Stigmella thibaulti Varenne & Nel, 2019 syn. nov. of Stigmella nivenburgensis (Preissecker, 1942) (Nepticulidae); Nemapogon peslieri Varenne & Nel, 2017 syn. nov. of Nemapogon inexpectata Varenne & Nel, 2017 (Tineidae); Phyllonorycter acericorsica Varenne & Nel, 2015 syn. nov. of Phyllonorycter ochreojunctella (Klimesch, 1942) (Gracillariidae); Ancylis paraobtusana Varenne, Nel, & Peslier, 2020 syn. nov. of Ancylis comptana (Frölich, 1828) (Tortricidae); Celypha paludicolella Varenne & Nel, 2017 syn. nov. of Celypha doubledayana (Barrett, 1872) (Tortricidae); Cydia oxytropidana Nel & Varenne, 2016 syn. nov. of Cydia oxytropidis (Martini, 1912) (Tortricidae); Sorhagenia orocorsa Varenne & Nel, 2016 syn. nov. of Sorhagenia janiszewskae Riedl, 1962 (Cosmopterigidae); Chionodes cerdanica Peslier, Nel & Varenne, 2020 syn. nov. of Chionodes distinctella (Zeller, 1839) (Gelechiidae); Elachista bidentata Varenne & Nel, 2019 syn. nov. of Elachista orstadii Palm, 1943; Elachista karsticola Varenne & Nel, 2018 syn. nov. of Elachista maculosella Chrétien, 1896 (Elachistidae); Scythris chablaisensis Delmas, 2018 syn. nov. of Scythris laminella ([Denis & Schiffermüller], 1775) (Scythrididae); Epermenia pumila (Buvat & Nel, 2000) syn. nov. of Epermenia profugella (Stainton, 1856) (Epermeniidae). Finally, the status of some taxa still remains unclear due to the lack of DNA barcodes of closely related species and the absence of convincing diagnostic characters in morphology. 


ZooKeys ◽  
2021 ◽  
Vol 1021 ◽  
pp. 1-18
Author(s):  
Dariusz Skarżyński ◽  
Adrian Smolis ◽  
Ľubomír Kováč ◽  
David Porco

A new species, Ceratophysella stachi, from Denmark, Germany, Luxembourg, Norway, Poland, and Ukraine is described based on morphological data and DNA barcodes. It belongs to a small European group of species with type B chaetotaxy and strong tegumentary granulation with distinct fields of coarse granules: C. granulata Stach, 1949, C. lawrencei (Gisin, 1963), C. neomeridionalis (Nosek & Červek, 1970), C. scotica (Carpenter & Evans, 1899), and C. silvatica Rusek, 1964. It differs from all of them in the chaetotaxy of lateral parts of thoracic terga II–III (setae m6 present and one additional seta outside lateral sensillum m7 present or absent) that is exceptional within the whole C. armata-group. Notes on closely related species C. granulata are also given.


2017 ◽  
Vol 9 (1) ◽  
pp. 9756 ◽  
Author(s):  
Bui Hong Quang ◽  
Vu Tien Chinh ◽  
Le Thi Mai Linh ◽  
Ritesh Kumar Choudhary

Two Jasminum taxa i.e. J. hongshuihoense and Jasminum laurifolium var. brachylobum are reported as a new record for the flora of Vietnam. J. hongshuihoense was hitherto known as endemic to China. It differs from the closely related species J. eberhardtii in having more number (4-10) of primary veins and lesser (5-8) calyx lobes. J. laurifolium var. brachylobum is closely allied to J. laurifolium var. laurifolium but it differs in having more flowers in cymes (i.e.1-8) flowers and calyx lobes (i.e. 6-10). Description and color photographs are provided for species identification. Furthermore, a comparison of the diagnostic characters with those of related species is made.


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