Species identification of four economically important Pangasiid catfishes and closely related species using SSCP markers

Aquaculture ◽  
2010 ◽  
Vol 308 ◽  
pp. S47-S50 ◽  
Author(s):  
Kednapat Sriphairoj ◽  
Sirawut Klinbu-nga ◽  
Wongpathom Kamonrat ◽  
Uthairat Na-Nakorn
Author(s):  
Antoni Lombarte ◽  
Marta M. Rufino ◽  
Pilar Sánchez

The statoliths of 14 species (193 right statoliths from subadult to adult individuals), belonging to five Cephalopoda families (Sepiidae, Loliginidae, Enoploteuthidae, Ommastrephidae and Octopodidae) were analysed using morphometric methods based on landmarks (geometric morphometry). The aim of the current study is to determine the discriminating power of statolith shape analysis in species identification of Mediterranean cephalopods. Discriminant analyses of the partial warps were able to fully identify (100% discrimination) the species of all families, except Octopodidae which showed some misclassification (correctly classified about 68–90%). These results were also shown by relative warp analysis. Octopodidae statoliths were studied for the first time using geometric landmark-based methods. Greatest differences in statolith shape between Octopodidae species, were in the area that unites the statolith dome with the flat wing. Landmark analysis applied to statoliths can be a useful taxonomic tool in the identification of closely related species.


Zootaxa ◽  
2017 ◽  
Vol 4365 (3) ◽  
pp. 311
Author(s):  
REZA GHADERI ◽  
LEILA KASHI ◽  
HOSSEIN MIRBABAEI KARANI ◽  
AKBAR KAREGAR

A new and four known species of the genus Diphtherophora—D. geraerti sp. n., D. caudata, D. obesa, D. perplexans and D. tenera—were collected and identified from different plants and localities in Iran. Morphological and morphometric characters of Diphtherophora geraerti sp. n. are compared with those of closely-related species, including D. siddiqii, D. brevicollis, D. lata, D. obesa, D. citri, D. vasilevi and D. vitoshae. Females of the new species have a head continuous with the body contour, spear 11–14 µm in length, rod-shaped spermatozoa and conical tail with bluntly rounded to hemispherical terminus. Males have two ventromedian neck papillae located 22–25 and 52–60 µm from the anterior end, and two ventromedian supplements at 11–13 and 75–80 µm anterior from cloacal aperture, respectively. All Diphtherophora species recovered as part of the present study are new records for the Iranian nematode fauna. Finally, a diagnostic compendium is given, to assist with species identification in the genus. 


Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1366
Author(s):  
Chufeng Zhao ◽  
Xuan She ◽  
Erxi Liu ◽  
Nunung Harijati ◽  
Teng Cheng ◽  
...  

Amorphophallus is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. Amorphophallus has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to Amorphophallus and closely related species. This study used six barcodes, namely ITS2, matK, rcbL, nad1, trnH-psbA, and trnL-trnF, to evaluate their identification ability for three important Amorphophallus species, including A. konjac, A. albus, and A. muelleri. We recommend that trnH-psbA can be applied to the Amorphophallus trade, quickly identify the purity of A. konjac and A. albus and distinguish A. muelleri from its related species for A. konjac and A. albus genetic improvement.


2017 ◽  
Vol 9 (1) ◽  
pp. 9756 ◽  
Author(s):  
Bui Hong Quang ◽  
Vu Tien Chinh ◽  
Le Thi Mai Linh ◽  
Ritesh Kumar Choudhary

Two Jasminum taxa i.e. J. hongshuihoense and Jasminum laurifolium var. brachylobum are reported as a new record for the flora of Vietnam. J. hongshuihoense was hitherto known as endemic to China. It differs from the closely related species J. eberhardtii in having more number (4-10) of primary veins and lesser (5-8) calyx lobes. J. laurifolium var. brachylobum is closely allied to J. laurifolium var. laurifolium but it differs in having more flowers in cymes (i.e.1-8) flowers and calyx lobes (i.e. 6-10). Description and color photographs are provided for species identification. Furthermore, a comparison of the diagnostic characters with those of related species is made.


2020 ◽  
Vol 9 (3) ◽  
pp. 160-170
Author(s):  
Thumadath P.A. Krishna ◽  
Maharajan Theivanayagam ◽  
Gurusunathan V. Roch ◽  
Veeramuthu Duraipandiyan ◽  
Savarimuthu Ignacimuthu

Finger millet is a superior staple food for human beings. Microsatellite or Simple Sequence Repeat (SSR) marker is a powerful tool for genetic mapping, diversity analysis and plant breeding. In finger millet, microsatellites show a higher level of polymorphism than other molecular marker systems. The identification and development of microsatellite markers are extremely expensive and time-consuming. Only less than 50% of SSR markers have been developed from microsatellite sequences for finger millet. Therefore, it is important to transfer SSR markers developed for related species/genus to finger millet. Cross-genome transferability is the easiest and cheapest method to develop SSR markers. Many comparative mapping studies using microsatellite markers clearly revealed the presence of synteny within the genomes of closely related species/ genus. Sufficient homology exists among several crop plant genomes in the sequences flanking the SSR loci. Thus, the SSR markers are beneficial to amplify the target regions in the finger millet genome. Many SSR markers were used for the analysis of cross-genome amplification in various plants such as Setaria italica, Pennisetum glaucum, Oryza sativa, Triticum aestivum, Zea mays and Hordeum vulgare. However, there is very little information available about cross-genome amplification of these markers in finger millet. The only limited report is available for the utilization of cross-genome amplified microsatellite markers in genetic analysis, gene mapping and other applications in finger millet. This review highlights the importance and implication of microsatellite markers such as genomic SSR (gSSR) and Expressed Sequence Tag (EST)-SSR in cross-genome analysis in finger millet. Nowadays, crop improvement has been one of the major priority areas of research in agriculture. The genome assisted breeding and genetic engineering plays a very crucial role in enhancing crop productivity. The rapid advance in molecular marker technology is helpful for crop improvement. Therefore, this review will be very helpful to the researchers for understanding the importance and implication of SSR markers in closely related species.


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