scholarly journals Tetraploidization Increases the Contents of Functional Metabolites in Cnidium officinale

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1561
Author(s):  
Hyung-Eun Kim ◽  
Jong-Eun Han ◽  
Hyoshin Lee ◽  
Ji-Hye Kim ◽  
Hyun-Hee Kim ◽  
...  
Keyword(s):  
Chromosome Doubling ◽  
Shoot Culture ◽  
Antioxidant Compounds ◽  
Stomatal Complex ◽  
Cnidium Officinale ◽  
Medicinal Crop ◽  
In Vitro Shoot Culture ◽  
Cytological Characteristics ◽  
Gene Expression Levels

Cnidium officinale is an important medicinal crop grown in Asia for its pharmacological properties. In this study, tetraploid breeding was conducted to increases the content of medicinal compound and tolerance to the environmental conditions using in vitro shoot culture of C. officinale. For this, we generated tetraploid C. officinale plants using oryzalin, a chromosome doubling agent, and compared the morphological traits, cytological characteristics, and heat stress-responsive gene expression levels between tetraploid and diploid genotypes. Chromosome doubling efficiency was the highest in plantlets treated with 4.0 mg∙L−1 oryzalin for 2 days. Compared with diploids, the plant height of tetraploids was reduced, while the petiole diameter was increased by approximately 39%. The dry matter of tetraploid leaves was significantly higher than that of diploid leaves. Compared with diploids, tetraploids showed higher chloroplast number and stomatal complex size but lower chlorophyll and carotenoid contents. The phenolic content of tetraploid plantlets was significantly higher than that of diploid plantlets. Contents of naringin as well as salicylic acid and gentisic acid, which are strong antioxidant compounds, were dramatically increased upon tetraploidization. Interestingly, liquid chromatography–mass spectrometry (LC–MS) analyses revealed increased levels of senkyunolide F and phthalide in tetraploid roots but not in tetraploid or diploid leaves.

In vitro shoot culture of Rhododendron fortunei: An important plant for bioactive phytochemicals

2018 ◽  
Vol 126 ◽  
pp. 459-465 ◽  
Author(s):  
Xiangying Wei ◽  
Jianjun Chen ◽  
Chunying Zhang ◽  
Zonghua Wang
Keyword(s):  
Shoot Culture ◽  
In Vitro Shoot Culture ◽  
Bioactive Phytochemicals

scholarly journals Antioxidants and Phenolic Secretion in Sugarcane Genotypes Shoots Culture

2018 ◽  
Vol 10 (5) ◽  
pp. 79
Author(s):  
Ana Da Silva Ledo ◽  
Maria M. Jenderek ◽  
Carlos Alberto Da Silva Ledo ◽  
Tomas Ayala-Silva
Keyword(s):  
Principal Component Analysis ◽  
Citric Acid ◽  
Phenolic Compounds ◽  
Shoot Culture ◽  
Shoot Proliferation ◽  
Principal Component ◽  
Shoot Formation ◽  
Regenerative Capacity ◽  
In Vitro Shoot Culture

Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.

Micropropagation of blueberry ‘Bluejay’ and ‘Pink Lemonade’ through in vitro shoot culture

2017 ◽  
Vol 226 ◽  
pp. 277-284 ◽  
Author(s):  
Shufang Fan ◽  
Dawei Jian ◽  
Xiangying Wei ◽  
Jianjun Chen ◽  
Richard C. Beeson ◽  
...  
Keyword(s):  
Shoot Culture ◽  
In Vitro Shoot Culture

Prevention of Vitrification Aßociated with in vitro Shoot Culture of Oregano. (Origanum vulgare) by Pseudomonas spp.

1995 ◽  
Vol 147 (3-4) ◽  
pp. 447-451 ◽  
Author(s):  
Kalidas Shetty ◽  
Otis F. Curtis ◽  
Robert E. Levin ◽  
Ruth Witkowsky ◽  
Woraluk Ang
Keyword(s):  
Shoot Culture ◽  
Origanum Vulgare ◽  
Pseudomonas Spp ◽  
In Vitro Shoot Culture

scholarly journals Micropropagation of Weigela florida ‘Tango’ through In Vitro Shoot Culture

HortScience ◽  
2017 ◽  
Vol 52 (2) ◽  
pp. 274-277 ◽  
Author(s):  
Xiaoming Wang ◽  
Yongxin Li ◽  
Huijie Zeng ◽  
Neng Cai ◽  
Zhongquan Qiao ◽  
...  
Keyword(s):  
Culture Media ◽  
Shoot Culture ◽  
Systematic Evaluation ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Axillary Shoots ◽  
Wide Range ◽  
In Vitro Shoot Culture ◽  
Weigela Florida

Weigela florida (Bunge) A. DC. is a popular flowering shrub adapted to a wide range of environmental conditions. Efficient methods for micropropagation of this species have not been well developed. The present study established a protocol for in vitro shoot culture of W. florida ‘Tango’ after a systematic evaluation of different culture media, cytokinins, and auxins on axillary shoot induction. Single-node stems were cultured on Driver and Kuniyuki Walnut (DKW) medium for initial production of axillary shoots. The shoots were used as explants and cultured on DKW medium supplemented with 8.88 μm 6-benzylaminopurine (BA) and 0.27 μm naphthaleneacetic acid (NAA), resulting in the production of more than six axillary shoots per explant. The axillary shoots could either be used as explants for additional shoot production or be cultured on ½ DKW medium supplemented with 0.25 μm indole-3-butyric acid (IBA) for rooting. Plantlets were transplanted into a substrate with 99% survival rate in a shaded greenhouse. This established method could be used for rapid propagation of W. florida to speed the introduction of new hybrids or cultivars for commercial production.

scholarly journals Analysis of elements and bacosides in in vitro shoot culture of Bacopa monnieri

2016 ◽  
Vol 38 (7) ◽  
Author(s):  
M. Łojewski ◽  
A. Krakowska ◽  
W. Reczyński ◽  
A. Szewczyk ◽  
B. Muszyńska
Keyword(s):  
Shoot Culture ◽  
Bacopa Monnieri ◽  
In Vitro Shoot Culture

scholarly journals Optimization of rhizogenesis for in vitro shoot culture of Pinus massoniana Lamb.

2019 ◽  
Vol 32 (1) ◽  
pp. 203-209 ◽  
Author(s):  
Yin Wang ◽  
Ruiling Yao
Keyword(s):  
Shoot Culture ◽  
Pinus Massoniana ◽  
Plantlet Regeneration ◽  
Naphthaleneacetic Acid ◽  
Root Number ◽  
Mature Trees ◽  
Rooting Medium ◽  
In Vitro Shoot Culture ◽  
Rooting Capacity

AbstractThe rooting capacity of Pinusmassoniana is poor, especially for mature trees, and has prevented the development of clonal forestry for P.massoniana. In this study, we varied explant types, subculture times and exogenous hormones for plantlet regeneration and assessed shoots for rooting rate and root number for P.massoniana. Following five repetitive grafts, new shoots from grafts used as explant sources were rejuvenated as observed from juvenile shoot morphology and anatomy, leading to greatly enhanced plant regeneration in comparison to that of mature materials from 26-year-old P.massoniana trees. The rooting capacity of subcultured shoots increased with successive subcultures, reaching a peak at 20 subcultures with 35–40 days per subculture. However, rooting performance was significantly reduced after 30 subcultures. The addition of naphthaleneacetic acid (NAA) plus indoleacetic acid in the medium improved the root number, but the combination of exogenous NAA with paclobutrazol (PBZ) increased rooting rate and root number. We thus greatly improved the rooting capacity of mature P.massoniana trees by optimizing explant types (rejuvenated), subculture times (20 subcultures, 35–40 days per subculture) and addition of NAA + PBZ to the rooting medium. The conditions can be used for efficient plantlet regeneration of P.massoniana.

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