scholarly journals Antioxidants and Phenolic Secretion in Sugarcane Genotypes Shoots Culture

2018 ◽  
Vol 10 (5) ◽  
pp. 79
Author(s):  
Ana Da Silva Ledo ◽  
Maria M. Jenderek ◽  
Carlos Alberto Da Silva Ledo ◽  
Tomas Ayala-Silva
Keyword(s):  
Principal Component Analysis ◽  
Citric Acid ◽  
Phenolic Compounds ◽  
Shoot Culture ◽  
Shoot Proliferation ◽  
Principal Component ◽  
Shoot Formation ◽  
Regenerative Capacity ◽  
In Vitro Shoot Culture

Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds.

scholarly journals Relationships Between the Content of Phenolic Compounds and the Antioxidant Activity of Polish Honey Varieties as a Tool for Botanical Discrimination

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1810
Author(s):  
Monika Kędzierska-Matysek ◽  
Małgorzata Stryjecka ◽  
Anna Teter ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Principal Component ◽  
Total Content ◽  
Component Analysis ◽  
Total Phenolic ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid

The study compared the content of eight phenolic acids and four flavonoids and the antioxidant activity of six Polish varietal honeys. An attempt was also made to determine the correlations between the antioxidant parameters of the honeys and their polyphenol profile using principal component analysis. Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (ABTS) and reduction capacity (FRAP) were determined spectrophotometrically, and the phenolic compounds were determined using high-performance liquid chromatography (HPLC). The buckwheat honeys showed the strongest antioxidant activity, most likely because they had the highest concentrations of total phenols, total flavonoids, p-hydroxybenzoic acid, caffeic acid, p-coumaric acid, vanillic acid and chrysin. The principal component analysis (PCA) of the data showed significant relationships between the botanic origin of the honey, the total content of phenolic compounds and flavonoids and the antioxidant activity of the six Polish varietal honeys. The strongest, significant correlations were shown for parameters of antioxidant activity and TPC, TFC, p-hydroxybenzoic acid, caffeic acid and p-coumaric acid. Analysis of four principal components (explaining 86.9% of the total variance), as a classification tool, confirmed the distinctiveness of the Polish honeys in terms of their antioxidant activity and content of phenolic compounds.

In vitro shoot culture of Rhododendron fortunei: An important plant for bioactive phytochemicals

2018 ◽  
Vol 126 ◽  
pp. 459-465 ◽  
Author(s):  
Xiangying Wei ◽  
Jianjun Chen ◽  
Chunying Zhang ◽  
Zonghua Wang
Keyword(s):  
Shoot Culture ◽  
In Vitro Shoot Culture ◽  
Bioactive Phytochemicals

scholarly journals Proximate Analysis and Nutritional Evaluation of Twenty Canadian Lentils by Principal Component and Cluster Analyses

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 175 ◽  
Author(s):  
D. Dan Ramdath ◽  
Zhan-Hui Lu ◽  
Padma L. Maharaj ◽  
Jordan Winberg ◽  
Yolanda Brummer ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Principal Components ◽  
Resistant Starch ◽  
Starch Content ◽  
Value Added ◽  
Principal Component ◽  
Proximate Analysis ◽  
Nutritional Properties ◽  
Nutritional Evaluation

Proximate composition and starch nutritional properties of twenty cooked lentils were assessed to identify unique varieties that could be used in value added foods. Significant variations exist among the lentil varieties (p < 0.05) with respect to their energy, fat, protein, carbohydrate, and dietary fiber content, and these are related to lentil type and seed size. Dazil and Greenstar were unique for their high resistant starch content (RS) and lower area under the starch hydrolysis curve (SHAUC) while Proclaim was opposite. SHAUC was positively correlated (p < 0.001) with rapidly digestible starch (RDS) content (r = 0.626) but negatively correlated with RS content (r = −0.635). Principal component analysis showed that the first three principal components accounted for 62.8% of the total variance and the contribution of SHAUC was 33.2%. These results confirm that in vitro SHAUC and a combination of RDS and RS may be predictive of the digestibility profile of cooked lentils.

Micropropagation of blueberry ‘Bluejay’ and ‘Pink Lemonade’ through in vitro shoot culture

2017 ◽  
Vol 226 ◽  
pp. 277-284 ◽  
Author(s):  
Shufang Fan ◽  
Dawei Jian ◽  
Xiangying Wei ◽  
Jianjun Chen ◽  
Richard C. Beeson ◽  
...  
Keyword(s):  
Shoot Culture ◽  
In Vitro Shoot Culture

Anti-inflammatory and anti-angiogenic activities in vitro of eight diterpenes from Daphne genkwa based on hierarchical cluster and principal component analysis

2018 ◽  
Vol 72 (3) ◽  
pp. 675-685
Author(s):  
Ling Wang ◽  
Xin-Yi Lan ◽  
Jun Ji ◽  
Chun-Feng Zhang ◽  
Fei Li ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Component Analysis ◽  
Anti Inflammatory ◽  
Daphne Genkwa

Prevention of Vitrification Aßociated with in vitro Shoot Culture of Oregano. (Origanum vulgare) by Pseudomonas spp.

1995 ◽  
Vol 147 (3-4) ◽  
pp. 447-451 ◽  
Author(s):  
Kalidas Shetty ◽  
Otis F. Curtis ◽  
Robert E. Levin ◽  
Ruth Witkowsky ◽  
Woraluk Ang
Keyword(s):  
Shoot Culture ◽  
Origanum Vulgare ◽  
Pseudomonas Spp ◽  
In Vitro Shoot Culture

scholarly journals INCREASED SHOOT PROLIFERATION OF APPLES CO-CULTIVATED WITH AGROBACTERIUM TUMEFACIENS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 661a-661
Author(s):  
F.A. Hammerschlag ◽  
R.H. Zimmerman ◽  
A.C. Smigocki
Keyword(s):  
Shoot Proliferation ◽  
Shoot Formation ◽  
Pcr Analysis ◽  
Axillary Shoot ◽  
Putative Transformants ◽  
Proliferation Medium ◽  
Shoot Production ◽  
Dna Fragment ◽  
Different Levels

`McIntosh' apple shoots were inoculated in vitro with Agrobacterium tumefaciens strain tms328::Tn5 (tms) carrying a functional cytokinin gene. Callus tissue, removed from the infected stems, produced shoots on shoot proliferation medium. After three subcultures, axillary shoot production from a tms-infected putative transformant was eight times that of controls. Subsequent shoot production on three different levels of BA (3, 6 and 10 uM) was significantly greater than from controls on all levels of BA. PCR analysis of putative transformants revealed an expected 503 bp DNA fragment corresponding to the amplified portion of the cytokinin gene. After 6 months of in vitro propagation, proliferation rates of shoots obtained from the original transformants were similar to the controls and the expected PCR fragment of 503 bp could only be detected by Southern analysis. Even though the T-DNA appears to be lost from the apple genome, the data suggest that the tms strain may be useful in co-infection experiments to induce shoot formation, thus avoiding difficult regeneration procedures.

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