scholarly journals Antimicrobial Resistance and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Slaughtered Pigs and Pork in the Central Region of Thailand

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 206
Author(s):  
Wimonrat Tanomsridachchai ◽  
Kanjana Changkaew ◽  
Ruchirada Changkwanyeun ◽  
Watsawan Prapasawat ◽  
Apiradee Intarapuk ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Nasal Swab ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Health Concern ◽  
Molecular Characteristics ◽  
P Value ◽  
Methicillin Resistant ◽  
Pork Samples ◽  
Slaughtered Pigs

Methicillin-resistant Staphylococcus aureus (MRSA) have been a major public health concern in humans. Among MRSA, livestock-associated (LA)-MRSA strains have always been associated with exposure to livestock or their products and have emerged in different countries globally. Although studies have identified LA-MRSA from healthy pigs and pork in Thailand, prevalence in slaughtered pigs is still unknown. In addition, there are few reports on the epidemiology and molecular characteristics of LA-MRSA in Thailand. Hence, this is the first report investigating the epidemiology and molecular characteristics of MRSA in individual slaughtered pigs and pork in Thailand. A total of 204 nasal swab and 116 retailed pork samples were collected from three slaughterhouses and four fresh markets, respectively. Individual samples were used for screening for MRSA and obtained isolates were examined for drug- resistance profiling for 12 antimicrobial agents of 10 drug classes. In addition, SCCmec typing and multi-locus sequence typing were conducted to obtain genotype profiles. MRSA were isolated from 11 and 52 nasal swab and pork samples, respectively. The prevalence was significantly higher in the pork than in the nasal swab samples (p-value < 0.05). A high prevalence of ST9-SCCmecIX and ST398-SCCmecV with high-level antimicrobial resistance from markets and slaughterhouses indicated the spreading of MRSA with these genotypes in the Thai swine processing chains and suggested the need for further investigation to determine a control.

Comparative Molecular Analysis of Meca, Sea and Seb Genes in Methicillin-Resistant Staphylococcus Aureus (MRSA)

2020 ◽  
pp. 1-8
Author(s):  
Ban A Alkhafaji and Ihsan Edan Alsaimary ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Nasal Swab ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Geographical Area ◽  
Antibiotic Resistant ◽  
Methicillin Resistant ◽  
The World ◽  
Hospital Wards

Methicillin resistant Staphylococcus aureus (MRSA), one of the most common pathogens associated with an increase of antimicrobial-resistance. Infections caused by Staphylococcus aureus may be demographically focused to specific parts of the world. This study will give critical bits of knowledge into the severity of the problem of antibiotic resistant organisms in Al-Basrah and give us valuable inputs regarding the possibility of a particular source being a contaminant that could lead to infection in this geographical area and study the profile of virulence genes including: (MecA, Sea, Seb).The study showed a high percentage of Staphylococcus aureus resistant to Methicillin, 39/46 (84.8%). that Nasal swab from H.C.Ws obtained the highest rate of MRSA 7/7 (100%) followed by wound swab 10/11 (90.9%) from patient most of them were already on antibiotic, Nasal swab from patient 5/6 (83%), swab taken from H.C.Ws hand and hospital wards 4/5 (80%) for each Methicillin Resistant Staphylococcus aureus obtained from Hospital theaters before sterilization and Skin swab from patient 3/4 (75%). MecA genes appeared in all MRSA isolates, Sea gene at 41% (16/39) and Seb genes, it was 10.3% (4/39).

scholarly journals Antimicrobial Resistance and Molecular Characteristics of Methicillin-resistant Staphylococcus aureus Isolates from Children Patients in Iran

2019 ◽  
Vol Volume 12 ◽  
pp. 3849-3857
Author(s):  
Roghayeh Samadi ◽  
Zhohreh Ghalavand ◽  
Reza Mirnejad ◽  
Bahram Nikmanesh ◽  
Gita Eslami
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Molecular Characteristics ◽  
Methicillin Resistant

scholarly journals Effectiveness of Methicillin-Resistant Staphylococcus aureus (MRSA) Nasal Screening for Reduction of Vancomycin Use for Pneumonia

2020 ◽  
Vol 41 (S1) ◽  
pp. s470-s471
Author(s):  
Shannon Snellgrove ◽  
Matthew Brown ◽  
Seth Edwards ◽  
Sixto Leal ◽  
Allen Bryan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nasal Swab ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Screening Tool ◽  
Hemodynamic Instability ◽  
Exclusion Criteria ◽  
Methicillin Resistant ◽  
Screening Questionnaire ◽  
Days Of Therapy ◽  
Antibiotic Coverage

Background: Methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization has been a well-established risk for developing MRSA pneumonia. In previous studies, the MRSA nasal screening test has shown an excellent negative predictive value (NPV) for MRSA pneumonia in patients without exclusion criteria such as mechanical ventilation, hemodynamic instability, cavitary lesions, and underlying pulmonary disease. MRSA nasal screening can be used as a stewardship tool to de-escalate broad antibiotic coverage, such as vancomycin. Objective: The purpose of this study was to determine whether implementation of a MRSA nasal screening questionnaire improves de-escalation of vancomycin for patients with pneumonia. Methods: A retrospective review was performed on 250 patients from October 2018 to January 2019 who received MRSA nasal screening due to their prescriber choosing only “respiratory” on the vancomycin dosing consult form. Data obtained included demographics and clinical outcomes. Statistical analyses were performed, and P < .05 was considered significant. Results: Of the 250 patients screened, only 19 patients (8%) were positive for MRSA. Moreover, 40% of patients met exclusion criteria. In 149 patients without exclusion criteria, the MRSA nasal swab had a 98% NPV. Although not statistically significant, vancomycin days of therapy (DOT) based on MRSA nasal swab result was 1 day shorter in those with negative swabs (3.49 days negative vs 4.58 days positive; P = .22). Vancomycin DOT was significantly reduced in pneumonia patients without exclusion criteria (3.17 days “no” vs 4.17 days “yes”; P = .037). Conclusions: The implementation of an electronic MRSA nasal screening questionnaire resulted in reduced vancomycin DOT in pneumonia patients at UAB Hospital. The MRSA nasal swab is an effective screening tool for antibiotic de-escalation based on its 98% NPV for MRSA pneumonia if utilized in the correct patient population.Funding: NoneDisclosures: Rachael Anne Lee reports a speaker honoraria from Prime Education, LLC.

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