scholarly journals Pharmacological Properties, Volatile Organic Compounds, and Genome Sequences of Bacterial Endophytes from the Mangrove Plant Rhizophora apiculata Blume

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1491
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Le Canh Viet Cuong ◽  
Le Tuan Anh ◽  
Le Thi Hong Minh ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Organic Compounds ◽  
Endophytic Bacteria ◽  
Pharmacological Properties ◽  
Bacillus Sp ◽  
Bacterial Endophytes ◽  
Mangrove Plant ◽  
Rhizophora Apiculata ◽  
Volatile Organic ◽  
Ethyl Acetate Extracts

Mangrove plant endophytic bacteria are prolific sources of bioactive secondary metabolites. In the present study, twenty-three endophytic bacteria were isolated from the fresh roots of the mangrove plant Rhizophora apiculata. The identification of isolates by 16S rRNA gene sequences revealed that the isolated endophytic bacteria belonged to nine genera, including Streptomyces, Bacillus, Pseudovibrio, Microbacterium, Brevibacterium, Microbulbifer, Micrococcus, Rossellomorea, and Paracoccus. The ethyl acetate extracts of the endophytic bacteria’s pharmacological properties were evaluated in vitro, including antimicrobial, antioxidant, α-amylase and α-glucosidase inhibitory, xanthine oxidase inhibitory, and cytotoxic activities. Gas chromatography–mass spectrometry (GC-MS) analyses of three high bioactive strains Bacillus sp. RAR_GA_16, Rossellomorea vietnamensis RAR_WA_32, and Bacillus sp. RAR_M1_44 identified major volatile organic compounds (VOCs) in their ethyl acetate extracts. Genome analyses identified biosynthesis gene clusters (BGCs) of secondary metabolites of the bacterial endophytes. The obtained results reveal that the endophytic bacteria from R. apiculata may be a potential source of pharmacological secondary metabolites, and further investigations of the high bioactive strains—such as fermentation and isolation of pure bioactive compounds, and heterologous expression of novel BGCs in appropriate expression hosts—may allow exploring and exploiting the promising bioactive compounds for future drug development.

Microalgal secondary metabolites: effect of climatic variables, seasons, and photocycles on the biogeneration of volatile organic compounds (VOCs)

2021 ◽  
Author(s):  
Rafaela Basso Sartori ◽  
Stefania Fortes Siqueira ◽  
Mariana Manzoni Maroneze ◽  
Mariane Bittencourt Fagundes ◽  
Roger Wagner ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Secondary Metabolites ◽  
Organic Compounds ◽  
Climatic Variables ◽  
Volatile Organic

scholarly journals Antibacterial Activity of Volatile Organic Compounds Produced by the Octocoral-Associated Bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 923
Author(s):  
Anette Garrido ◽  
Librada A. Atencio ◽  
Rita Bethancourt ◽  
Ariadna Bethancourt ◽  
Héctor Guzmán ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Resistant Bacteria ◽  
Bacillus Sp ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Associated Bacteria ◽  
Volatile Organic

The present research aimed to evaluate the antibacterial activity of volatile organic compounds (VOCs) produced by octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327. The volatilome bioactivity of both bacteria species was evaluated against human pathogenic antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus, Acinetobacter baumanni, and Pseudomonas aeruginosa. In this regard, the in vitro tests showed that Bacillus sp. BO53 VOCs inhibited the growth of P. aeruginosa and reduced the growth of S. aureus and A. baumanni. Furthermore, Pseudoalteromonas sp. GA327 strongly inhibited the growth of A. baumanni, and P. aeruginosa. VOCs were analyzed by headspace solid-phase microextraction (HS-SPME) joined to gas chromatography-mass spectrometry (GC-MS) methodology. Nineteen VOCs were identified, where 5-acetyl-2-methylpyridine, 2-butanone, and 2-nonanone were the major compounds identified on Bacillus sp. BO53 VOCs; while 1-pentanol, 2-butanone, and butyl formate were the primary volatile compounds detected in Pseudoalteromonas sp. GA327. We proposed that the observed bioactivity is mainly due to the efficient inhibitory biochemical mechanisms of alcohols and ketones upon antibiotic-resistant bacteria. This is the first report which describes the antibacterial activity of VOCs emitted by octocoral-associated bacteria.

scholarly journals Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa

2020 ◽  
Vol 41 (4) ◽  
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Vu Thi Thanh Tam ◽  
Hoang Le Tuan Anh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Antioxidant Agents ◽  
Antimicrobial And Antioxidant Activity ◽  
Ethyl Acetate Extracts

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.       

Volatile Organic Compounds Produced by Cacao Endophytic Bacteria and Their Inhibitory Activity on Moniliophthora roreri

2022 ◽  
Vol 79 (2) ◽  
Author(s):  
Norma De la Cruz-López ◽  
Leopoldo Cruz-López ◽  
Francisco Holguín-Meléndez ◽  
Griselda Karina Guillén-Navarro ◽  
Graciela Huerta-Palacios
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Inhibitory Activity ◽  
Endophytic Bacteria ◽  
Volatile Organic ◽  
Moniliophthora Roreri

scholarly journals \(\textit{In vitro}\) antioxidant, α-amylase and α-glucosidase inhibitory activities of endophytic bacteria from the roots of the mangrove plant \(\textit{Rhizophora stylosa}\) Griffith

2021 ◽  
Vol 43 (3) ◽  
pp. 125-135
Author(s):  
Ton That Huu Dat ◽  
Oanh Phung Thi Thuy
Keyword(s):  
Microbial Communities ◽  
Endophytic Bacteria ◽  
Biological Molecules ◽  
Bacterial Endophytes ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Inhibitory Activities

Mangrove is one of the highly productive ecosystems and contains diverse plants and microbial communities. Bacterial endophytes from mangroves are considered as a prolific source of biological molecules with important functions in the protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate endophytic bacteria from the roots of mangrove plant Rhizophora stylosa and to screen antioxidant,

scholarly journals Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Vu Thi Thanh Tam ◽  
Hoang Le Tuan Anh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Antioxidant Agents ◽  
Antimicrobial And Antioxidant Activity ◽  
Ethyl Acetate Extracts

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.       

scholarly journals Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

2009 ◽  
Vol 66 (5) ◽  
pp. 583-592 ◽  
Author(s):  
Flávia Mandolesi Pereira de Melo ◽  
Marli Fátima Fiore ◽  
Luiz Alberto Beraldo de Moraes ◽  
Maria Estela Silva-Stenico ◽  
Shirlei Scramin ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Bacteria ◽  
Bacillus Pumilus ◽  
Sclerotium Rolfsii ◽  
Antifungal Compound ◽  
Fame Analysis ◽  
Cell Tissue ◽  
Rrna Sequencing ◽  
Indian Tribes ◽  
Ethyl Acetate Extracts

In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy.

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