Assessment cytotoxic assay of Rhizophora plants mangrove using brine shrimp (Artemia salina L) model

Rhizophoraceae is the main family of mangroves as a source of bioactive compounds originating from the coast. Ethnophamacologically Rhizophoraceae has been used in various traditional medicine. Natural sources as anticancer from the Rhizophoraceae family are interesting to know. This study aimed to determine the cytotoxic bioactivity of methanolic extracts of roots, bark, leaves, and fruit/hypocotyl from five species of Rhizophoraceae (Bruguieria cylindrica, B. gymnorrhiza, Ceriops decandra, Rhizophora apiculata, and R. mucronata) from the Langsa mangrove forest, Aceh. The method used in this study was the Brine Shrimp Lethality Test (BSLT) bioassay using Artemia salina Leach at extract concentrations of 1, 10, 100, 500, and 1000 μg/ml. Samples were extracted using the maceration method and methanol as the solvent. The cytotoxic activity of 20 Rhizophoraceae methanol extracts showed that 12 extracts were toxic with an LC50 range of 31.5 - 934.9 μg/ml (based on LC50 ≤ 1000 μg/ml). The two extracts of which the closest to highly toxic (based on LC50 ≤ 30 μg/ml) were C. decandra bark showed LC50 of 31.5 μg/ml, and R. mucronata bark showed LC50 31.8 μg/ml. This shows that Rhizophoraceae extract has potential as a natural anticancer agent. In the five rhizophoraceae species, C. decandra was the most active compared to other species. In the four plant parts, the bark was the most toxic.

Mangrove Ecosystem Biodiversity in Malacca Strait and Its Economic Values to Local Community: A Case Study in Riau Coastline

The mangrove ecosystem holds a lot of potentials to be utilized by local communities in the Riau Coastline. Therefore, this study was carried out to assess the biodiversity of flora and fauna in the Riau Coastline mangrove ecosystem and its impacts on local communities. The transect was performed to identify soil conditions, distribution, and the dominance of flora species, while field observations and interviews with residents were conducted to determine the presence of fauna and discover the ecosystem’s benefit to the local community's economy. Based on the results, Rhizophora mucronata and Rhizophora apiculata has the highest relative dominance (RDC) and important value index in all observed stations. On the other hand, the fish/water biota species has the highest diversity and have economic that mostly serve as a catch for local fishermen. Ecotourism is also encouraged to boost the community's economy and aid ecological learning for sustainability purposes. The mangrove ecosystem's existence remains sustainable because fisheries are the main commodity, however, logging is less desirable as a source of income.

Bioakumulasi Merkuri (Hg) pada Lamun Enhalus acoroides dan Mangrove Rhizophora apiculata di Pulau Pari, Kepulauan Seribu

<strong>Bioaccumulation of Mercury (Hg) in Seagrass <em>Enhalus acoroides</em> and Mangrove <em>Rhizophora apiculata</em> in Pari Island, Seribu Islands. </strong>Heavy metal pollution, especially Hg metal in Jakarta Bay, is feared to have a negative impact on the quality of the waters around the Jakarta Bay waters, one of which is Pari Island in the Thousand Islands. Seagrass and mangroves plants distributed surrounding Pari Island can be used as indicators of heavy metal pollution. The value of bioaccumulation and translocation of Hg metal factors will provide an overview of the ability of plants to accumulate heavy metals in their surroundings. This study aims to determine the bioaccumulation and translocation of the heavy metal Hg factor in <em>Enhalus acoroides</em> seagrass and <em>Rhizophora apiculata</em> mangroves on Pari Island, Seribu Islands. Seagrass and mangrove samples were taken at five sampling points through purposive sampling. The bioaccumulation value was calculated using the ratio of the metal concentrations in each part of the plant body and sediment. Meanwhile, the translocation factor value was calculated by the ratio of Hg concentration in each part of the plant body (roots/rhizomes, stems, and leaves). The results of this study indicate that the bioaccumulation value in seagrass is infinite (bioaccumulation factor, BAF=∞) and in mangroves is 1.57 (BAF&gt;1). The highest <em>translocation factor</em> value in seagrass leaves is 3.86 (translocation factor, TF) &gt;1) and in mangrove leaves is 2.84 (TF&gt;1). This study shows that seagrass and mangrove plants are classified as good bio accumulator and hyperaccumulator plants and accumulate heavy metals, especially Hg in the upper part of their bodies, namely leaves

Struktur Komunitas Vegetasi Mangrove Di Pesisir Pantai Kepulauan Karimunjawa

Mangrove merupakan tumbuhan yang berada di wilayah intertidal pesisir laut. Tujuan penelitian ini yaitu menganalisis struktur vegetasi mangrove (frekuensi, kerapatan, dominan) dan karakteristik habitatnya (kualitas lingkungan) di kawasan pesisir pantai kepulauan Karimunjawa. Penelitian dilakukan pada Desember 2019 di 3 stasiun dengan metode plot bertingkat, masing-masing stasiun dibuat 3 transek yang berukuran 10m x 10m (pohon), 5m x 5m (pancang), dan 2m x 2m (semai). Hasil penelitian ditemukan 7 jenis mangrove yaitu Rhizophora stylosa, Avicennia marina, Rhizophora apiculata, Ceriops tagal, Excoecaria agallocha, Lumnitzera racemosa, dan Ceriops decandra. Indeks nilai penting tumbuhan mangrove pada strata pohon, pancang, dan semai paling tinggi adalah Rhizopora stylosa (244,77%), (163,03%), dan (157,96%). Nilai kerapatan Rhizopora stylosa tingkat pohon, tingkat pancang dan semai yaitu (2.500-10.100 ind/ha), (10.400-48.400 ind/ha), dan (97.500-280.000 ind/ha). Kondisi lingkungan di sekitar kawasan mangrove yaitu rata-rata suhu (28,75%), pasir (10,75%), lanau (51,46%/), lempung (37,79%), salinitas (26,60%), pH (7,26), DO (3,28 mg/L), N total tanah (0,24%), P total tanah (120,49 ppm), C Organik tanah (2,10%), N total air (0,28%), P total air (0,27 mg/L), C Organik air (1,56 mg/L).ABSTRACTMangroves are a plant that are found in the intertidal area of marine coastal environments. The study aim to analyze structure of mangrove vegetation (frequency, density, and dominance) and the mangrove habitat (environmental condition) in Coastal Coast Karimunjawa Island. The research was conducted in December 2019 at the three stations using the stratified plot method, and one stations divided three observation transects sized 10m x 10m (trees), 5m x 5m (saplings), and 2m x 2m (seedlings). The result of the study found seven mangroves species were Rhizophora stylosa, Avicennia marina, Rhizophora apiculata, Ceriops tagal, Excoecaria agallocha, Lumnitzera racemosa, and Ceriops decandra. The highest value index of mangroves for trees, saplings and seedlings is the highest Rhizopora stylosa (244,77%), (163,03%), and (157,96%). Density value Rhizopora stylosa in tree level, saplings, and seedlings were (2.500-10.100 ind/ha), (10.400-48.400 ind/ha), dan (97.500-280.000 ind/ha). The environmental conditions around the mangrove area are average temperature (28,75%), sand (10,75%), silt (51,46%/), clay (37,79%), salinity (26,60%), pH (7,26), Dissolved Oxygen (3,28 mg/L), N total land (0,24%), P total land (120,49 ppm), C Organic land (2,10%), N total water (0,28%), P total water (0,27 mg/L), C Organic water (1,56 mg/L).

Temperature Stress on Physiological and Morphological Traits in Rhizophora apiculata

Global warming has had considerable effects on vital ecosystems, which has also been caused by increased temperatures and CO2 that follow changes in different abiotic factors, which poses threats to mangrove forests environment. This research was conducted to examine the physiological and morphological characteristics of the Rhizophora apiculata mangrove regarding higher air temperature for the variety of tree species that respond to climate change. Seedlings were cultivated for three months in regulated growth chambers with three varying temperatures of 38°C, 21°C under CO2 at 450 ppm, and ambient CO2 concentration i.e., 450 ± 20 ppm under average temperature at 28°C as the control condition. The plants were treated every 48 hours with 3 L of saline water of 28 ppt. After two weeks at high temperature, the mangrove showed positive results for all parameters. The temperature variations resulted in major variations, such as negative for increased temperature resulting in extreme damage to many samples while positive for decreased temperature resulting in slow development. The physiological results show decreased photosynthesis rates compared to controlled samples. These findings indicate that low photosynthetic capability levels could have occurred due to reduced CO2 fixative reaction mechanism, photosynthetic pigment material, and the discrepancy between respiratory and photosynthesis rate.

The Mangrove Landscape and Zonation following Soil Properties and Water Inundation Distribution in Segara Anakan Cilacap

The mangrove zoning and landscape express the correlation between mangrove vegetation (density, biodiversity and species distribution) with environment factors like as water inundation, seatide, and soil properties. The research was conducted in Segara Anakan Lagoon to analysis community structure and mangrove landscape based on species distribution, biodiversity, environment factors, and mangrove zoning. The results showed that (a) Segara Anakan Lagoon had 4 mangrove zone's were dominated by Sonneratia alba, Rhizophora mucronata, Avicennia marina, Rhizophora apiculata, Rhizophora styllosa, and Nypa frutican; (b) the structure of ecosystem was showed by trend of mangrove ecosystem with equation y = 35.34x2 923.85x + 12817 with x = time (year) and y = mangrove area (ha), mangrove density between 1333367 ind ha-1 (West Segara Anakan) and 899–567 ind ha-1 (East Segara Anakan), dominated species were Nypa frutican, Rhizophora stylosa, Rhizophora apiculata, and Aegiceras corniculatum and mangrove biodiversity between 2,572,65 (moderate); (c) structure of environment factor showed single and semi double-type tides, water debit between 0.360.73 m s-1; water depth between 0.20–23.7 m and water inundation between 480cm; soil texture was clay and loam, soil nitrate of 1.5 mg 100 g-1, soil phosphate of 1.5 mg 100 g-1, C organic of 1.31%, soil pH of 6–7, and soil salinity of 6.5–10 ppt.

Characteristics of Microbial Community and Function With the Succession of Mangroves

In this study, 16S high-throughput and metagenomic sequencing analyses were employed to explore the changes in microbial community and function with the succession of mangroves (Sonneratia alba, Rhizophora apiculata, and Bruguiera parviflora) along the Merbok river estuary in Malaysia. The sediments of the three mangroves harbored their own unique dominant microbial taxa, whereas R. apiculata exhibited the highest microbial diversity. In general, Gammaproteobacteria, Actinobacteria, Alphaproteobacteria, Deltaproteobacteria, and Anaerolineae were the dominant microbial classes, but their abundances varied significantly among the three mangroves. Principal coordinates and redundancy analyses revealed that the specificity of the microbial community was highly correlated with mangrove populations and environmental factors. The results further showed that R. apiculata exhibited the highest carbon-related metabolism, coinciding with the highest organic carbon and microbial diversity. In addition, specific microbial taxa, such as Desulfobacterales and Rhizobiales, contributed the highest functional activities related to carbon metabolism, prokaryote carbon fixation, and methane metabolism. The present results provide a comprehensive understanding of the adaptations and functions of microbes in relation to environmental transition and mangrove succession in intertidal regions. High microbial diversity and carbon metabolism in R. apiculata might in turn facilitate and maintain the formation of climax mangroves in the middle region of the Merbok river estuary.