scholarly journals Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Vu Thi Thanh Tam ◽  
Hoang Le Tuan Anh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Antioxidant Agents ◽  
Antimicrobial And Antioxidant Activity ◽  
Ethyl Acetate Extracts

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.       

scholarly journals Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa

2020 ◽  
Vol 41 (4) ◽  
Author(s):  
Ton That Huu Dat ◽  
Phung Thi Thuy Oanh ◽  
Vu Thi Thanh Tam ◽  
Hoang Le Tuan Anh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Endophytic Bacteria ◽  
Radical Scavenging ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Antioxidant Agents ◽  
Antimicrobial And Antioxidant Activity ◽  
Ethyl Acetate Extracts

Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.       

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals \(\textit{In vitro}\) antioxidant, α-amylase and α-glucosidase inhibitory activities of endophytic bacteria from the roots of the mangrove plant \(\textit{Rhizophora stylosa}\) Griffith

2021 ◽  
Vol 43 (3) ◽  
pp. 125-135
Author(s):  
Ton That Huu Dat ◽  
Oanh Phung Thi Thuy
Keyword(s):  
Microbial Communities ◽  
Endophytic Bacteria ◽  
Biological Molecules ◽  
Bacterial Endophytes ◽  
Mangrove Plant ◽  
Rhizophora Stylosa ◽  
Mangrove Plants ◽  
Inhibitory Activities

Mangrove is one of the highly productive ecosystems and contains diverse plants and microbial communities. Bacterial endophytes from mangroves are considered as a prolific source of biological molecules with important functions in the protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate endophytic bacteria from the roots of mangrove plant Rhizophora stylosa and to screen antioxidant,

scholarly journals An Evaluation of The Antifungal and Antioxidant Activity of Pinus merkusii Bark Ethyl Acetate Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 102-110
Author(s):  
Masendra Masendra ◽  
Brandon Aristo Verick Purba Purba ◽  
Ganis Lukmandaru
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Growth Inhibition ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Compounds ◽  
Potential Source ◽  
Antioxidant Agents ◽  
Pinus Merkusii ◽  
Ethyl Acetate Extracts

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.

scholarly journals ASSESSING COSMECEUTICALS PROPERTIES OF SOME MACROFUNGI FOR IMPROVED SKINCARE

2019 ◽  
pp. 15-19
Author(s):  
AKASH SARASWAT ◽  
PURVI MATHUR ◽  
DOYELI SANYAL
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Sun Protection ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Protection Factor ◽  
Sun Protection Factor ◽  
Ethyl Acetate Extracts

Objective: This study has investigated cosmeceutical properties namely, antibacterial, sun protection factor and total phenolics contents of some selected macrofungi. The studies were conducted on five reference cultures collected from Indian type culture collection center (IMTEC, Chandigarh, India) and five isolates collected from TERI-Deakin Nano Biotechnology Centre facility at TERI Gram, Gurugram, India. Methods: The cosmeceutical properties of the crude extracts from selected macrofungi were analyzed using standard bioassay techniques. Antibacterial activity was analyzed against Staphylococcus epidermidis, Escherichia coli, Micrococcus luteus, Bacillus megaterium, Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii using Agar well diffusion method. The sun protection factor was estimated and calculated using the Mansur equation. Free radical scavenging activity using DPPH was performed to assess the antioxidant activity of the extracts. Results: Ethyl acetate extracts of the broth from P. florida and TERI-G1 cultures showed a broad-spectrum antibacterial activity against S. epidermidis, E. coli, M. luteus and B. megaterium. Ethyl acetate extracts of the broth from TERI-G3 showed the highest SPF activity of 34.02 at 200 µg. ml-1 concentration. Ethyl acetate extract of the broth from F. velutipes, P. florida, P. ostreatus, and TERI-G1 showed comparable antioxidant activity of 66.86%, 79.51%, 82.02%, and 69.58% respectively when compared to ascorbic acid (85.83%) and quercetin (83.09%) taken as positive control in the study and their total phenolic contents were found to be 6.93, 43.68, 20.88 and 13.77 Gallic acid equivalent (GAE) per gram. The minimal inhibitory concentration of F. velutipes, P. florida, P. ostreatus, and TERI-G1 was found to be 3552.89 µg. ml-1, 1250 µg. ml-1, 2418.9 µg. ml-1 and 3219 µg. ml-1 respectively. Conclusion: The work is in progress to identify and characterize TERI-G1and TERI G3 cultures. Further studies on the anti-inflammatory, anti-tyrosinase, elastase inhibition properties of the cultures will be assessed to identify potential cosmeceutical active ingredients with promising applications in cosmeceutical products.

scholarly journals Effect of Extraction Solvent on Total Phenol, Flavonoid Content, and Antioxidant Activity of Avicennia Officinalis

2021 ◽  
Vol 12 (2) ◽  
pp. 2678-2690
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Extraction Process ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Mangrove Plant ◽  
Avicennia Officinalis ◽  
Phenolic And Flavonoid ◽  
Radical Scavenging Assay

The mangrove plant - Avicennia officinalis L has been widely used for the treatment of various diseases such as anti-inflammatory, anticancer, antioxidant therapy. In this study, we optimize the extraction process using the easily obtainable solvents with different polarities toward obtaining high total phenolic- and flavonoid- contents from Avicennia officinalis L's leaves. The influences of solvents (i.e., methanol, ethanol, ethyl acetate, acetone, dichloromethane, and chloroform) in the extraction process on total phenolic-, flavonoid- contents and their antioxidant activity were investigated. Avicennia officinalis L was extracted using six solvents. The total phenolic and flavonoid contents were determined by the phytochemical screening method. The antioxidant activity of extracted phenolics and flavonoids was studied by using 2,2 diphenyl 1 picrylhydrazy (DPPH) radical scavenging assay and 2,2 azinobis (3 ethylbenzothiazoline 6 sulfonate) (ABTS+) radical scavenging assay. As a result, the highest extraction yield was obtained using methanol (10.56%). In addition, the extract obtained using acetone solvent exhibited the highest antioxidant activity in the DPPH assay. Meanwhile, the extract obtained using ethyl acetate solvent showed the highest antioxidant activity in the ABTS+ assay. The acetone- extract also exhibited high phenolic content (100.64 mg GAE/g) and flavonoid content (95.44 mg QCE/g). This study confirms that, besides commonly used solvents such as methanol, ethanol, etc., acetone is also a good solvent for extracting polyphenols from the mangrove plant - Avicennia officinalis L. for antioxidants.

Synthesis and antioxidant activity of new selenium-containing quinolines

2020 ◽  
Vol 16 ◽  
Author(s):  
Benedetta Bocchini ◽  
Bruna Goldani ◽  
Fernanda S.S. Sousa ◽  
Paloma T. Birmann ◽  
Cesar A. Brüning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Radical Scavenging ◽  
Building Blocks ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Pharmacological Activities ◽  
Antioxidant Power ◽  
Antioxidant Agents

Background: Quinoline derivatives have been attracted much attention in drug discovery and synthetic derivatives of these scaffolds present a range of pharmacological activities. Therefore, organoselenium compounds are valuable scaffolds in organic synthesis because their pharmacological activities and their use as versatile building blocks for regio-, chemio-and stereoselective reactions. Thus, the synthesis of selenium-containing quinolines has great significance, and their applicability range from simple antioxidant agents, to selective DNA-binding and photocleaving agents. Objective: In the present study we describe the synthesis and antioxidant activity in vitro of new 7-chloroN(arylselanyl)quinolin-4-amines 5 by the reaction of 4,7-dichloroquinoline 4 with (arylselanyl)-amines 3. Methods: For the synthesis of 7-chloro-N(arylselanyl)quinolin-4-amines 5, we performed the reaction of (arylselanyl)- amines 3 with 4,7-dichloroquinoline 4 in the presence of Et3N at 120 °C in a sealed tube. The antioxidant activities of the compounds 5 were evaluated by the following in vitro assays: 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric ion reducing antioxidant power (FRAP), nitric oxide (NO) scavenging and superoxide dismutase-like activity (SOD-Like). Results: 7-Chloro-N(arylselanyl)quinolin-4-amines 5a-d has been synthesized in yields ranging from 68% to 82% by the reaction of 4,7-dichloroquinoline 4 with arylselanyl-amines 3a-d using Et3N as base, at 120 °C, in a sealed tube for 24 hours and tolerates different substituents, such as -OMe and -Cl, in the arylselanyl moiety. The obtained compounds 5a-d presented significant results with respect to the antioxidant potential, which had effect in the tests of inhibition of radical’s DPPH, ABTS+ and NO, as well as in the test that evaluates the capacity (FRAP) and in the superoxide dismutase-like activity assay (SOD-Like). It is worth mentioning that 7-chloro-N(arylselanyl)quinolin-4-amine 5b presented excellent results, demonstrating a better antioxidant capacity when compared to the others. Conclusion: According to the obtained results 7-chloro-N(arylselanyl)quinolin-4-amines 5 were synthesized in good yields by the reaction of 4,7-dichloroquinoline with arylselanyl-amines and tolerates different substituents in the arylselanyl moiety. The tested compounds presented significant antioxidant potential in the tests of inhibition of DPPH, ABTS+ and NO radicals, as well as in the FRAP and superoxide dismutase-like activity assays (SOD-Like).

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

scholarly journals Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2555
Author(s):  
Sang Koo Park ◽  
Yoon Kyung Lee
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyphenol Content ◽  
Abts Assay ◽  
Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

scholarly journals Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

2014 ◽  
Vol 9 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Mirjana Marčetić ◽  
Silvana Petrović ◽  
Marina Milenković ◽  
Marjan Niketić
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Volatile Fraction ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Antimicrobial And Antioxidant Activity

AbstractThe chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

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