scholarly journals The Pro-Oxidant Activity of Red Wine Polyphenols Induces an Adaptive Antioxidant Response in Human Erythrocytes

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 800
Author(s):  
Idolo Tedesco ◽  
Carmela Spagnuolo ◽  
Gian Luigi Russo ◽  
Maria Russo ◽  
Carmen Cervellera ◽  
...  
Keyword(s):  
Red Wine ◽  
Redox System ◽  
Underlying Mechanism ◽  
Antioxidant Response ◽  
Human Erythrocytes ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Oxidant Effect

The protective effect of dealcoholized red wine on human health has been partially associated with its polyphenolic components, suggesting that the pool of polyphenols, including flavonoids and anthocyanins, can be responsible for the functional effects of this beverage. We hypothesize a new role of red wine polyphenols (RWp) in modulating the antioxidant potential of erythrocytes, protecting them against oxidative stress. We previously demonstrated that RWp activated the Plasma Membrane Redox System (PMRS), which is involved in neutralizing plasma free radicals. Here, we investigated the underlying mechanism triggered by RWp in the activation of PMRS via the involvement of GSH. Hence, treatment of human erythrocytes with RWp (73 μg/mL Gallic Acid Equivalents) increased GSH intracellular concentration, which depends upon the activation of glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD), whose enzymatic activities increase of about 30% and 47%, respectively. Changes in the GSH pathway induced by RWp were associated with a slight but significant increase in reactive oxygen species (ROS). We conclude that the pro-oxidant effect of RWp promoted an adaptive stress response in human erythrocytes, which enhances their antioxidant defense.

Role of endothelial cell membrane transport in red wine polyphenols-induced coronary vasorelaxation: involvement of bilitranslocase

2013 ◽  
Vol 4 (10) ◽  
pp. 1452 ◽  
Author(s):  
Lovro Ziberna ◽  
Jong-Hun Kim ◽  
Cyril Auger ◽  
Sabina Passamonti ◽  
Valérie Schini-Kerth
Keyword(s):  
Endothelial Cell ◽  
Cell Membrane ◽  
Membrane Transport ◽  
Red Wine ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols ◽  
Cell Membrane Transport

Role of gender and estrogen receptors in the rat aorta endothelium-dependent relaxation to red wine polyphenols

2009 ◽  
Vol 51 (2-3) ◽  
pp. 140-146 ◽  
Author(s):  
Modou Oumy Kane ◽  
Eric Anselm ◽  
Yanna Dantas Rattmann ◽  
Cyril Auger ◽  
Valérie B. Schini-Kerth
Keyword(s):  
Estrogen Receptors ◽  
Red Wine ◽  
Rat Aorta ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols ◽  
Endothelium Dependent Relaxation

Apoptotic effect of red wine polyphenols on human colon cancer SNU-C4 cells

2006 ◽  
Vol 44 (6) ◽  
pp. 898-902 ◽  
Author(s):  
M.-J. Kim ◽  
Y.-J. Kim ◽  
H.-J. Park ◽  
J.-H. Chung ◽  
K.-H. Leem ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Red Wine ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Apoptotic Effect ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols

Angiotensin II Induces the Vascular Expression of VEGF and MMP-2 in vivo: Preventive Effect of Red Wine Polyphenols

2008 ◽  
Vol 45 (5) ◽  
pp. 386-394 ◽  
Author(s):  
Allison Walter ◽  
Nelly Etienne-Selloum ◽  
Mamadou Sarr ◽  
Modou Oumy Kane ◽  
Alain Beretz ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Red Wine ◽  
Preventive Effect ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols ◽  
Vascular Expression

Endothelium-derived contracting factors mediate the Ang II-induced endothelial dysfunction in the rat aorta: preventive effect of red wine polyphenols

2009 ◽  
Vol 459 (5) ◽  
pp. 671-679 ◽  
Author(s):  
Modou O. Kane ◽  
Nelly Etienne-Selloum ◽  
Soccoro V. F. Madeira ◽  
Mamadou Sarr ◽  
Allison Walter ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Red Wine ◽  
Rat Aorta ◽  
Ang Ii ◽  
Preventive Effect ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols

scholarly journals eNOS Promoter Activation by Red Wine Polyphenols: an Interaction Study

2013 ◽  
Vol 60 (1) ◽  
pp. 27-33 ◽  
Author(s):  
E. Kurin ◽  
N. Fakhrudin ◽  
M. Nagy
Keyword(s):  
Reporter Gene ◽  
Red Wine ◽  
Interaction Analysis ◽  
Mass Action ◽  
Luciferase Reporter ◽  
Interaction Study ◽  
Luciferase Reporter Gene ◽  
Gene Assay ◽  
Wine Polyphenols ◽  
Red Wine Polyphenols

Beneficial effects of red wine polyphenols on cardiovascular health are well known. The aim of our research was an interaction study of four red wine polyphenols – resveratrol (R), quercetin (Q), kaempferol (KF) and isorhamnetin (IR) of their ability to activate endothelial NO synthase (eNOS) promoter when used alone and in equimolar mixtures. To determine their activity, we performed a luciferase reporter gene assay on EA.hy926 cells stably transfected with a luciferase reporter gene construct containing eNOS promoter. The Bradford assay was also performed to account the cytotoxicity and/or the cell number differences. The median effect equation, as an interaction analysis evaluating synergy or antagonism of the combinations was done according to mass-action law principle. Isobolographic method was performed on selected double mixtures and dose reduction index was calculated for all mixtures. All single polyphenols activated eNOS promoter. The EC50 values were in micromolar concentrations ranging from 3.44 μM (R2 = 0.96) for kaempferol to 9.89 μM for isorhamnetin (R2 = 0.94). All mixtures activated eNOS promoter, but their interactions varied from synergy (Q+R, Q+IR+KF, Q+R+KF and Q+R+IR+KF), through additive (R+IR+KF) to antagonistic interaction (R+IR, R+KF, Q+IR, Q+KF, IR+KF and R+Q+IR). In this study, we show for the first time that red wine polyphenols activated eNOS promoter when used alone and in mixtures with different type of interactions.

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