Vepris macrophylla Essential Oil Produces Notable Antiproliferative Activity and Morphological Alterations in Human Breast Adenocarcinoma Cells

Medicinal plants contain numerous bioactive molecules that synergistically provide therapeutic benefits. We have devoted our attention to various EOs without toxicity to normal cells, studying their activities against human cancer cells. In particular, we have studied the cytotoxicity of Vepris macrophylla (Baker) I. Verd. EO. V. macrophylla is an evergreen tree of Madagascar where is much appreciated as a source of traditional remedies. Its major volatile components are citral, i.e., a mixture of neral and geranial, citronellol and myrcene. The antiproliferative activities of V. macrophylla EO were studied against human breast adenocarcinoma cell line SKBR3. Cellular metabolism was analyzed by MTT assay at different concentrations of EO and at different times of incubation (24, 48 and 72 h). Moreover, morphological and ultrastructural analyses were performed to study its antiproliferative effects against human adenocarcinoma cells, demonstrating the ability of V. macrophylla EO, stored inside numerous intracellular vesicles, to damage both plasma membranes and disorganize the cytoskeleton protein as actin filaments.

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Change in the Expression of BAK، FAS، BAX، TNF-A، BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v27i6.1602 ◽

2019 ◽

Author(s):

Sara Afzali ◽

Abbas Doosti ◽

Mansour Heidari ◽

Nahid Babaei ◽

Parvaneh Keshavarz

Keyword(s):

Staphylococcal Enterotoxin ◽

Breast Adenocarcinoma ◽

Control Group ◽

Staphylococcal Enterotoxin B ◽

Type B ◽

Human Breast ◽

Human Breast Adenocarcinoma ◽

Adenocarcinoma Cells ◽

Enterotoxin B ◽

Mcf 7

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-2 و Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells. Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7 cells transfected with the pcDNA3.1(+)-seb (recombinant) and pcDNA3.1(+) (non-recombinant) plasmids and were selected by culturing in a selective medium of RPMI- 1640 containing 600 μg / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used. Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032). Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.

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