scholarly journals Initial Characterization of PDMAEMA: Styrene Porous Polymer Monolithic Morphologies

2021 ◽  
Vol 11 (15) ◽  
pp. 7097
Author(s):  
Madisyn Hayes ◽  
Alyssa Smith ◽  
Corbin Arrasmith ◽  
Willow Davis ◽  
Charlisa R. Daniels
Keyword(s):  
Small Molecules ◽  
Capillary Electrochromatography ◽  
Stationary Phases ◽  
Porous Polymer ◽  
Dynamic Studies ◽  
Initial Characterization ◽  
Stimuli Response ◽  
Membrane Technologies ◽  
External Stimuli

This research aimed to investigate a new fabrication of poly-(2-dimethyl(aminoethyl) methacrylate)-co-styrene in a porous polymer monolithic morphology. Poly-(2-dimethyl(aminoethyl) methacrylate) (PDMAEMA) is responsive to changes in pH while styrene remains unresponsive to external stimuli. IR, UV/Vis, and SEM were employed to determine that the proposed porous polymer (PPM) monolith can present pH-initiated stimuli response while remaining intact. The copolymerization of PDMAEMA with styrene has not been previously documented in a PPM morphology. It is important to demonstrate that the PPM retains tunable capabilities without destruction to the material. The utility of PDMAEMA copolymers is wide-reaching and this new adaptation of its tunability in a resilient PPM can serve as a distinct preface to original applications in fields such as surface modification, membrane technologies and stationary phases. To further the groundwork of this communication, dynamic studies on the interactions of small molecules with the pores of the monolith will be essential and accomplished via capillary electrochromatography.

scholarly journals Identification and initial characterization of POLIII-driven transcripts by msRNA-sequencing.

RNA Biology ◽  
2021 ◽  
Author(s):  
Peter Zorn ◽  
Danny Misiak ◽  
Michael Gekle ◽  
Marcel Köhn
Keyword(s):  
Initial Characterization

scholarly journals Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C

2021 ◽  
Vol 11 (4) ◽  
pp. 1941
Author(s):  
Seungmin Yeom ◽  
Myung Chul Lee ◽  
Shambhavi Pandey ◽  
Jaewoon Lim ◽  
Sangbae Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Stem Cell ◽  
Vitamin C ◽  
Suspension Culture ◽  
Small Molecules ◽  
Tumor Development ◽  
Embryonic Stem ◽  
Short Term ◽  
External Stimuli

Induced pluripotent stem cells (iPSCs) are a good medicine source because of their potential to differentiate into various tissues or cells. However, traditionally, iPSCs made by specific transgenes and virus vectors are not appropriate for clinical use because of safety concerns and risk of tumor development. The goal of this research was to develop an alternative method for reprogramming, using small molecules and external stimuli. Two groups were established: short-term shear stress (STSS) under suspension culture and a combination of short-term shear stress and vitamin C (SSVC) under suspension culture. For STSS, the pipetting was carried out for cells twice per day for 2 min for 14 days in the embryonic stem cell (ES) medium. In the case of SSVC, the procedure was the same as for STSS however, its ES medium included 10 µM of vitamin C. After 14 days, all spheroids were picked and checked for pluripotency by ALP (alkaline phosphatase) assay and immunocytochemistry. Both groups partially showed the characteristics of stem cells but data demonstrated that the spheroids under shear stress and vitamin C had improved stem cell-like properties. This research showed the possibility of external stimuli and small molecules to reprogram the somatic cells without the use of transgenes.

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