scholarly journals Effects of Germination Black Soy Milk Fermented with Lactobacillus plantarum TWK10 on Anti-Oxidative and Anti-Melanogenesis

2021 ◽  
Vol 12 (1) ◽  
pp. 277
Author(s):  
Te-Hua Liu ◽  
Wei-Ting Chiang ◽  
Meng-Chun Cheng ◽  
Tsung-Yu Tsai
Keyword(s):  
Lactobacillus Plantarum ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Soy Milk ◽  
Melanin Content ◽  
Flavonoid Content ◽  
Ethanol Extracts ◽  
Gallic Acid Equivalent

Black soybean germination or fermentation increases active ingredient bioavailability and anti-oxidative activity. This study investigated the effects of fermented and germinated black soy milk on anti-oxidation and melanogenesis inhibition. The total phenolic content (TPC; 42.66 ± 1.65 mg gallic acid equivalent/g) and total flavonoid content (TFC; 5.43 ± 0.54 mg quercetin equivalent (QE)/g) in ethanol extracts from Lactobacillus plantarum TWK10 (TWK10)-fermented nongermination black soy milk (FNGB) were significantly (p < 0.05) higher than those in ethanol extracts from nonfermented-nongermination black soy milk (NNGB). Although the TPC of ethanol extracts from nonfermented-germination black soy milk (NGB) and fermented-germination black soy milk (FGB) were not significantly different (p > 0.05), the TFC of FGB (1.79 ± 0.08 mg QE/g) was significantly higher than that of NGB (p < 0.05). The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of ethanol extracts from NNGB, FNGB, NGB, and FGB was significantly higher than that of water extracts at 10 mg/mL (p < 0.05). Furthermore, ethanol extracts from both FNGB and FGB significantly reduced the melanin content in zebrafish embryos at 100 µg/mL (p < 0.05) without causing death, malformation or bradycardia. Overall, the antioxidant activity of black soy milk significantly increased after TWK10 fermentation; moreover, ethanol extracts from FNGB and FGB could inhibit melanogenesis, indicating their potential as whitening ingredients.

scholarly journals WATER EXTRACTS FROM THE COMBINATION OF COCCINIA GRANDIS (L.) VOIGT LEAVES AND AVERRHOA BILIMBI L. FRUITS WITH ANTIDIABETIC PROPERTIES: AN IN VITRO STUDY

2020 ◽  
pp. 24-28
Author(s):  
MADE WISNU ADHI PUTRA I ◽  
OLYN TIEN ATE ◽  
GUSTI AYU WITA KUSUMAWATI I ◽  
WAYAN NURSINI NI
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Water Extracts ◽  
Coccinia Grandis ◽  
Gallic Acid Equivalent

Objective: The combination of two or more plants in a product can improve the nature of the activity of the compounds contained and reduce toxicity. This study was aimed to make water extracts from the combination of Coccinia grandis (L.) CG Voigt and Averrhoa bilimbi L. (AB) with antidiabetic properties. Methods: The water extracts were made by decocting the combination of powdered CG Voigt leaves and AB fruits at various mass ratios (1:0, 1:3, 1:1, 3:1, and 0:1) and various amounts of powdered samples (1–5 g). The process was done in 100 ml of boiling water for 5 min. Results: The results showed that the highest total flavonoid content and total phenolic content of 135.1±0.18 mg quercetin equivalent micrograms d.m. and 48.5±3.09 mg gallic acid equivalent/g d.m., respectively, were found in the sample with a combination ratio of 1:3 (1 g). The antioxidant activity test using the 2,2-diphenyl-1-picrylhydrazyl method showed that the sample with a combination ratio of 3:1 had the lowest value of IC50 (4.447±0.446 mg/ml), which indicated the strongest radical scavenging activity, while the highest inhibition of α-amylase activity in the sample with a combination ratio of 1:1 amounted to 1.086±0.264 mg/ml. Conclusion: In general, all combinations of CG Voigt leaves and AB fruits had the potential as antidiabetic health beverages.

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

scholarly journals Evaluation antioxidant capacity and proximate composition in brown seaweed S. crassifolium found in Lombok coast, Indonesia

2021 ◽  
Vol 913 (1) ◽  
pp. 012042
Author(s):  
N Ardiana ◽  
A S Abidin ◽  
B T K Ilhami ◽  
A L Sunarwidhi ◽  
S Widyastuti ◽  
...  
Keyword(s):  
Proximate Composition ◽  
Total Phenolic Content ◽  
Functional Food ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Abstract Seaweed is one of the marine-biota that is widely known for its uses, one of them is in the food functional. Functional food is a program that promotes a healthy diet so that it can prevent a disease when consuming food. With functional food, daily eating patterns can be maintained and also the content of the food consumed daily has a good effect on the body. It is known that in seaweed, especially brown algae (S. crassifolium), it contains antioxidants and other compounds that are good for the health. In this study, phytochemical content and proximate composition was evaluated in S. crassifolium. Including total flavonoid content and total phenolic content, antioxidant capacity both of DPPH and ABTS assay used three type of solvents such as Ethanol, n-Hexana, and ethyl acetate for comparable the best solvents. Also, proximate analysis to determine the content of moisture content, crude fat content, crude protein content, and carbohydrates content. The results showed that n-Hexana and ethyl acetate solvents were significantly than ethanol. Which mean both of them had highest values of total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity. The results of chemical composition in proximate compounds showed that S. crassifolium was suitable to be candidate of functional food.

scholarly journals Antioxidant, Anti-Inflammatory and Antibacterial Activities of the Seeds of A Sri Lankan Variety of Carica Papaya

2019 ◽  
Vol 12 (2) ◽  
pp. 539-547
Author(s):  
Akshani Anjula Wijesooriya ◽  
Srianthie A. Deraniyagala ◽  
Chamari M. Hettiarachchi
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Sri Lankan ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Anti Inflammatory

Plant based remedies are of much importance in healthcare due to low side effects. The effective constituents contained in seeds of a papaya have not been utilized efficiently in the production of medicines. The purpose of this study was driven towards determining the total phenolic content, total flavonoid content, antioxidant capacity, anti-inflammatory activity and antibacterial properties of the aqueous seeds extract of a Sri Lankan variety (Red Lady) of papaya (AESP). The AESP prepared according to the method of “Kasaya” in Ayurvedic medicine was used for the investigations according to standard procedures. Total phenolic content and the total flavonoid content of the AESP were 13.5±2.2 mg (pyrogallol equivalence)/g and 315.9±104.6 mg (quercetin equivalence)/g respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of AESP ranged from 12.4% to 81.2% for concentrations of 1.0-20.0 mg/ml; ascorbic acid gave 31.7% - 91.9% activity. The AESP displayed strong reducing power compared to ascorbic acid in the reducing power assay. The hydroxyl radical scavenging activity of the AESP ranged from 6.6% to 69.1% for concentrations ranging from 20.0-95.0 mg/ml; ascorbic acid activity was 37.7%-74.9%. The nitric oxide radical scavenging activity of AESP was 23.9%-62.7% for concentrations of 2.0-20.0 µg/ml; ascorbic acid gave 26.8%-63.5% activity. AESP concentrations ranging from 75.0-150.0 µg/ml exhibited human red blood cell (HRBC) membrane stabilization protection of 15.5%-22.7% compared to 50.8%-58.4% for aspirin. The AESP showed antibacterial activity against Bacillus subtilis and Staphylococcus aureus bacterial species. AESP possess promising antioxidant, anti-inflammatory and antibacterial activity. The aqueous decoction of the discarded seeds of papaya would facilitate remedies for many diseases in which radicals are implicated as well as assist against certain bacterial infections and also has an anti-inflammatory potential.

scholarly journals Correlation between total flavonoid content and total phenolic content on antioxidant activity of ethanol extracts from three cultivars of papaya leaves

2020 ◽  
Vol 11 (2) ◽  
pp. 1883-1887
Author(s):  
Prakit Chaithada ◽  
Praweena Whenngean ◽  
Radchada Fungfueng ◽  
Saowanee Maungchanburee
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ethanol Extracts ◽  
Dpph Radical Scavenging

The aim of this study was to elucidate the correlation between total flavonoid content and total phenolic content on antioxidants activity of ethanol extracts from three cultivars of papaya leaves: 'Holland,' 'Khak Dam' and 'Red Lady.' All crude extracts were investigated to find antioxidant capacity in DPPH radical scavenging. The result indicated that the ethanol extract of ‘Red Lady’ papaya leaves exhibited the highest level of DPPH radical scavenging activity with the IC50 of 0.18 mg/mL, followed by the ‘Khak Dam’ and ‘Holland’ papaya leaves having an IC50 value of 0.24 and 0.44 mg/mL, respectively. The ethanol extract from 'Red Lady' papaya leaves showed the highest level of total flavonoid content (TFC) of 276.72 ± 1.04 µgQE/g DW and total phenolic content (TPC) of 169.85 ± 6.54 mgGAE/g DW. All three cultivars showed a distinctive correlation between IC50 and the content of both total flavonoid and total phenolic with a negative relationship of Pearson’s correlation of -0.922 and -0.940, respectively.

scholarly journals Phenolic content and antioxidant activity of Echinocystis lobata (Mich.) Torr. ET Gray (Cucurbitaceae)

10.5219/1579 ◽  
2021 ◽  
Vol 15 ◽  
pp. 784-791
Author(s):  
Yulia Vinogradova ◽  
Olga Shelepova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Jan Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Echinocystis Lobata

Echinocystis lobata (Mich.) Torr. et Gray extracts were investigated for their bioactive compounds and their potential for natural antioxidant properties. In vitro antioxidant activity of methanol, ethanol, and water extracts were evaluated by 1,1-diphenyl-picrylhydrazyl (DPPH) radical scavenging activity. Total phenolic content (TPC) and total flavonoid content (TFC) of fruits and seeds were determined. TPC is a fairly constant value and varies weakly both among the different organs of Echinocystis lobata and among the geographical locations of the populations studied. Like other invasive species, Echinocystis lobata is superior to many other plants in terms of the total amount of flavonoids contained in its fruits and seeds. The dried fruits contain more flavonoids than the seeds. Seeds from Slovak populations have higher flavonoid content than those from Russian populations. The results demonstrated that Echinocystis lobata can be a good source of natural antioxidants and have a great potential for commercialization, especially in the pharmaceutical industry.

scholarly journals Evaluation of Phenolic Content and Free Radical Scavenging Activity of Indonesia Wild Honey Collected from Seven Different Regions

2019 ◽  
Vol 8 (6) ◽  
pp. 94
Author(s):  
Y. Riswahyuli ◽  
Abdul Rohman ◽  
Francis M. C. S. Setyabudi ◽  
Sri Raharjo
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content

Several wild honey samples collected from seven different regions in Indonesia were investigated to determine their total phenolic content, flavonoid content, and free radical scavenging activity by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DDPH) radical and phenolic profile. Rutin, (+)-catechin, ferulic acid, and galangin were found to be the major phenolic compounds of Indonesia wild honey. The total phenolic content significantly correlated with the total flavonoid content (p=0.000) and the percentage of DPPH radical scavenging activity (p=0.000). Results indicated that there are different polyphenol profiles among the different regions.

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