scholarly journals Application of Microgel as a Sorbent for Bisphenol Analysis in Liquid Food Samples

2022 ◽  
Vol 12 (1) ◽  
pp. 441
Author(s):  
Anna Kubiak ◽  
Marcin Maćkiewicz ◽  
Marcin Karbarz ◽  
Magdalena Biesaga

Bisphenols are well-known endocrine disruptors that can easily migrate from plastic and can containers to food. Due to the complicated matrix and ultra-low concentrations of bisphenols in food, samples require extensive preparation before instrumental analysis. In this paper, an environmental sensitive microgel was employed as a sorbent for the preconcentration of four bisphenols, bisphenol A (BPA), bisphenol B (BPB), bisphenol E (BPE) and bisphenol F (BPF), from liquid food samples. Liquid chromatography with fluorescence detection (LC-FLD) was used for the quantification of bisphenols. By applying microgel solid-phase extraction procedure, the limits of detections achieved in liquid food samples can be lowered to 0.9 µg·L−1 for BPF and BPA, 2.3 µg·L−1 for BPE and 2.9 µg·L−1 for BPB. Only 5 mg of microgel was sufficient to achieve good recoveries (70.5–109%) with precision (RSD 0.21–5.01%, n = 3) for different analyzed liquid food samples spiked at concentration levels of 50 µg·L−1. In five out of twelve of the analyzed samples (pineapple, mandarin, peach, mushroom and pickles), they were contaminated with BPA, and the determined concentration was in the range of 6.2–22 µg·L−1; however, these results are below the specific migration limit (SML) set for BPA (50 µg·kg−1).

1997 ◽  
Vol 43 (2) ◽  
pp. 312-325 ◽  
Author(s):  
Chi-Kong Lai ◽  
Ting Lee ◽  
Kam-Ming Au ◽  
Albert Yan-Wo Chan

Abstract In this HPLC–diode-array detection method for toxicological drug screening, a mixed-mode solid-phase extraction procedure is optimized for isolation of a broad range of drugs from serum and urine. Basic, neutral, and weakly acidic drugs are uniformly recovered. The extract from the solid-phase cartridge is readily injected to a reversed-phase HPLC column for separation by gradient elution. Unknown drugs and metabolites in urine and serum samples from acute drug poisoning cases are rapidly identified by matching their retention times and ultraviolet spectra with hundreds of reference compounds in the library. Urine metabolites of common toxicants from various medications and drugs of abuse are recorded, with their changes of retention times and ultraviolet spectra as related to their metabolic transformations. Glucuronide conjugates of common benzodiazepines, tricyclic antidepressants, and beta-blockers are examined directly without chemical or enzymatic hydrolysis. The system is reliable for diverse clinical investigations of drug overdoses, drug-induced psychoses, and substance abuse.


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