scholarly journals Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide–Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes

2019 ◽  
Vol 9 (2) ◽  
pp. 326 ◽  
Author(s):  
Somasekhar Chinnadayyala ◽  
Jinsoo Park ◽  
Yonghyun Choi ◽  
Jae-Hee Han ◽  
Ajay Yagati ◽  
...  

The improved binding ability of graphene–nanoparticle composites to proteins or molecules can be utilized to develop new cell-based assays. In this study, we fabricated reduced graphene oxide–gold nanoparticles (rGO-AuNP) electrodeposited onto a transparent indium tin oxide (ITO) electrode and investigated the feasibility of the electrochemical impedance monitoring of cell growth. The electrodeposition of rGO–AuNP on the ITO was optically and electrochemically characterized in comparison to bare, rGO-, and AuNP-deposited electrodes. The cell growth on the rGO–AuNP/ITO electrode was analyzed via electrochemical impedance measurement together with the microscopic observation of HEK293 cells transfected with a green fluorescent protein expression vector. The results showed that rGO–AuNP was biocompatible and induced an increase in cell adherence to the electrode when compared to the bare, AuNP-, or rGO-deposited ITO electrode. At 54 h cultivation, the average and standard deviation of the saturated normalized impedance magnitude of the rGO–AuNP/ITO electrode was 3.44 ± 0.16, while the value of the bare, AuNP-, and rGO-deposited ITO electrode was 2.48 ± 0.15, 2.61 ± 0.18, and 3.01 ± 0.25, respectively. The higher saturated value of the cell impedance indicates that the impedimetric cell-based assay has a broader measurement range. Thus, the rGO–AuNP/ITO electrode can be utilized for label-free and real-time impedimetric cell-based assays with wider dynamic range.

RSC Advances ◽  
2014 ◽  
Vol 4 (104) ◽  
pp. 60386-60396 ◽  
Author(s):  
Pratima R. Solanki ◽  
Saurabh Srivastava ◽  
Md. Azahar Ali ◽  
Rajesh Kr. Srivastava ◽  
Anchal Srivastava ◽  
...  

A label-free biosensor has been fabricated using a reduced graphene oxide and anatase titania nanocomposite, deposited on indium tin oxide electrode for the specific recognition of Vibrio cholerae.


2014 ◽  
Vol 14 (5) ◽  
pp. 738-743 ◽  
Author(s):  
Thuy Kieu Truong ◽  
T.N.T. Nguyen ◽  
Tran Quang Trung ◽  
Il Yung Sohn ◽  
Duck-Jin Kim ◽  
...  

2012 ◽  
Vol 468-471 ◽  
pp. 1823-1826 ◽  
Author(s):  
Ya Nan Wang ◽  
Peng Fei Gu ◽  
Jia Jia Cao ◽  
Tian Quan Lv ◽  
Tie Qiang Zhang ◽  
...  

As indium tin oxide (ITO) can not meet the demands of many devices due to its fragility, graphene seems to be a good alternative for transparent conductor purpose. Here we employ two methods to prepare graphene oxide films, and then process them with hydroiodic acid (HI) for reduction, aiming to get an optimized scheme and higher film quality. Finally the reduced graphene oxide films with transmittance of 70~80% at 550nm show sheet resistance of <10kΩ, which are good enough for transparent conductor uses. This study portends a promising future in this field.


Sign in / Sign up

Export Citation Format

Share Document