scholarly journals Synthesis and Characterization of Controlled Nitric Oxide Release from S-Nitroso-N-Acetyl-d-Penicillamine Covalently Linked to Polyvinyl Chloride (SNAP-PVC)

2018 ◽  
Vol 5 (3) ◽  
pp. 72 ◽  
Author(s):  
Sean Hopkins ◽  
Megan Frost
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Response ◽  
Polyvinyl Chloride ◽  
Light Emitting ◽  
Nitric Oxide Release ◽  
Passive Mechanism ◽  
Controllable Release ◽  
Covalently Linked ◽  
Nitric Oxide Releasing

Polyvinyl chloride (PVC) is one of the most widely used polymers in medicine but has very poor biocompatibility when in contact with tissue or blood. To increase biocompatibility, controlled release of nitric oxide (NO) can be utilized to mitigate and reduce the inflammatory response. A synthetic route is described where PVC is aminated to a specified degree and then further modified by covalently linking S-nitroso-N-acetyl-d-penicillamine (SNAP) groups to the free primary amine sites to create a nitric oxide releasing polymer (SNAP-PVC). Controllable release of NO from SNAP-PVC is described using photoinitiation from light emitting diodes (LEDs). Ion-mediated NO release is also demonstrated as another pathway to provide a passive mechanism for NO delivery. The large range of NO fluxes obtained from the SNAP-PVC films indicate many potential uses in mediating unwanted inflammatory response in blood- and tissue-contacting devices and as a tool for delivering precise amounts of NO in vitro.

scholarly journals New nitric oxide-releasing indomethacin derivatives with 1,3-thiazolidine-4-one scaffold: Design, synthesis, in silico and in vitro studies

2021 ◽  
Vol 139 ◽  
pp. 111678
Author(s):  
Alexandru Sava ◽  
Frederic Buron ◽  
Sylvain Routier ◽  
Alina Panainte ◽  
Nela Bibire ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Silico ◽  
In Vitro Studies ◽  
Scaffold Design ◽  
Design Synthesis ◽  
Nitric Oxide Releasing

Nitric Oxide-Donating Derivatives of Chrysin Stimulate Angiogenesis and Upregulating VEGF Production

2011 ◽  
Vol 340 ◽  
pp. 363-368 ◽  
Author(s):  
Xiao Qing Zou ◽  
Yong Lan Ding ◽  
Sheng Ming Peng ◽  
Chang Ping Hu ◽  
Han Wu Deng ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Therapeutic Option ◽  
Therapeutic Angiogenesis ◽  
Vegf Mrna ◽  
No Donor ◽  
Endothelial Migration ◽  
Nitric Oxide Releasing ◽  
Derivatives Of

Angiogenesis, the development of new capillaries from pre-existing vessels, requires the coordinate activation of endothelial cells, which migrate and proliferate to form functional vessels. Endothelial dysfunction and decreased nitric oxide bioavailability may underscore the impairment of angiogenesis. As such, the delivery of exogenous NO is an attractive therapeutic option that has been used to therapeutic angiogenesis. In this paper, a novel group of hybrid nitric oxide-releasing chrysin derivatives was synthesized. The results indicated that all these chrysin derivatives exhibited promotion of endothelial migration and tubulogenesis in vitro as well as stimulation angiogenesis in vivo.Furthermore, all compounds released NO upon incubation with phosphate buffer at pH 7.4 and enhanced VEGF secretion and VEGF mRNA expression of endothelial cells. These hybrid ester NO donor prodrugs offer a potential drug design concept for the development of therapeutic or preventive agents for angiogenesis deficiency due to ischemic diseases.

scholarly journals 536 In vitro and in vivo efficacy of nitric oxide-releasing antiviral therapeutic agents

2016 ◽  
Vol 136 (5) ◽  
pp. S95
Author(s):  
K.A. McHale ◽  
K. Balogh ◽  
H. Wang ◽  
S. Hollenbach ◽  
N. Christensen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Therapeutic Agents ◽  
In Vivo Efficacy ◽  
Nitric Oxide Releasing

Nitric oxide-releasing porous materials and their potential regulation of in-vitro biological functions

2018 ◽  
Vol 120 ◽  
pp. S151
Author(s):  
Rosana Pinto ◽  
Cristina Fernandes ◽  
João Pires ◽  
Fernando Antunes ◽  
João Rocha ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Porous Materials ◽  
Biological Functions ◽  
Nitric Oxide Releasing

Nitric Oxide Releasing Polyurethanes with Covalently Linked Diazeniumdiolated Secondary Amines

2006 ◽  
Vol 7 (3) ◽  
pp. 987-994 ◽  
Author(s):  
Melissa M. Reynolds ◽  
Joseph A. Hrabie ◽  
Bong K. Oh ◽  
Jeffrey K. Politis ◽  
Michael L. Citro ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Secondary Amines ◽  
Covalently Linked ◽  
Nitric Oxide Releasing

scholarly journals Tylosema esculentum(Marama) Tuber and Bean Extracts Are Strong Antiviral Agents against Rotavirus Infection

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Walter Chingwaru ◽  
Runner T. Majinda ◽  
Sam O. Yeboah ◽  
Jose C. Jackson ◽  
Petrina T. Kapewangolo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Seed Coat ◽  
Antiviral Agents ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Human Infant ◽  
Nitric Oxide Release ◽  
Water Extracts ◽  
Tylosema Esculentum

Tylosema esculentum(marama) beans and tubers are used as food, and traditional medicine against diarrhoea in Southern Africa. Rotaviruses (RVs) are a major cause of diarrhoea among infants, young children, immunocompromised people, and domesticated animals. Our work is first to determine anti-RV activity of marama bean and tuber ethanol and water extracts; in this case on intestinal enterocyte cells of human infant (H4), adult pig (CLAB) and adult bovine (CIEB) origin. Marama cotyledon ethanolic extract (MCE) and cotyledon water extract (MCW) without RV were not cytotoxic to all cells tested, while seed coat and tuber extracts showed variable levels of cytotoxicity. Marama cotyledon ethanolic and water extracts (MCE and MCW, resp.) (≥0.1 mg/mL), seed coat extract (MSCE) and seed coat water extract (MSCW) (0.01 to 0.001 mg/mL), especially ethanolic, significantly increased cell survival and enhanced survival to cytopathic effects of RV by at least 100% after in vitro co- and pre-incubation treatments. All marama extracts used significantly enhanced nitric oxide release from H4 cells and enhanced TER (Ω/cm2) of enterocyte barriers after coincubation with RV. Marama cotyledon and seed coat extracts inhibited virion infectivity possibly through interference with replication due to accumulation of nitric oxide. Marama extracts are therefore promising microbicides against RV.

scholarly journals Nitric Oxide-Releasing Nanoparticles Are Similar to Efinaconazole in Their Capacity to Eradicate Trichophyton rubrum Biofilms

2021 ◽  
Vol 11 ◽  
Author(s):  
Caroline Barcelos Costa-Orlandi ◽  
Luis R. Martinez ◽  
Níura Madalena Bila ◽  
Joel M. Friedman ◽  
Adam J. Friedman ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Trichophyton Rubrum ◽  
Fungal Infections ◽  
Antifungal Drugs ◽  
New Drugs ◽  
Prolonged Treatment ◽  
Clinical Strain ◽  
Causative Agents ◽  
Nitric Oxide Releasing

Filamentous fungi such as Trichophyton rubrum and T. mentagrophytes, the main causative agents of onychomycosis, have been recognized as biofilm-forming microorganisms. Nitric oxide-releasing nanoparticles (NO-np) are currently in development for the management of superficial and deep bacterial and fungal infections, with documented activity against biofilms. In this context, this work aimed to evaluate, for the first time, the in vitro anti-T. rubrum biofilm potential of NO-np using standard ATCC MYA-4438 and clinical BR1A strains and compare it to commonly used antifungal drugs including fluconazole, terbinafine and efinaconazole. The biofilms formed by the standard strain produced more biomass than those from the clinical strain. NO-np, fluconazole, terbinafine, and efinaconazole inhibited the in vitro growth of planktonic T. rubrum cells. Similarly, NO-np reduced the metabolic activities of clinical strain BR1A preformed biofilms at the highest concentration tested (SMIC50 = 40 mg/mL). Scanning electron and confocal microscopy revealed that NO-np and efinaconazole severely damaged established biofilms for both strains, resulting in collapse of hyphal cell walls and reduced the density, extracellular matrix and thickness of the biofilms. These findings suggest that biofilms should be considered when developing and testing new drugs for the treatment of dermatophytosis. Development of a biofilm phenotype by these fungi may explain the resistance of dermatophytes to some antifungals and why prolonged treatment is usually required for onychomycosis.

scholarly journals Development of Antimicrobial Nitric Oxide-Releasing Fibers

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1445
Author(s):  
Daniel C. Wang ◽  
Justin R. Clark ◽  
Richard Lee ◽  
Adam H. Nelson ◽  
Anthony W. Maresso ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Release Kinetics ◽  
Antimicrobial Properties ◽  
No Donor ◽  
Long Term Stability ◽  
Effective Delivery ◽  
Culture Models ◽  
Delivery Platform ◽  
Nitric Oxide Releasing

Nitric oxide (NO) is a highly reactive gas molecule, exhibiting antimicrobial properties. Because of its reactive nature, it is challenging to store and deliver NO efficiently as a therapeutic agent. The objective of this study was to develop NO-releasing polymeric fibers (NO-fibers), as an effective delivery platform for NO. NO-fibers were fabricated with biopolymer solutions of polyvinyl pyrrolidone (PVP) and ethylcellulose (EC), and derivatives of N-diazeniumdiolate (NONOate) as NO donor molecules, using an electrospinning system. We evaluated in vitro NO release kinetics, along with antimicrobial effects and cytotoxicity in microorganisms and human cell culture models. We also studied the long-term stability of NONOates in NO-fibers over 12 months. We demonstrated that the NO-fibers could release NO over 24 h, and showed inhibition of the growth of Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA), without causing cytotoxicity in human cells. NO-fibers were able to store NONOates for over 12 months at room temperature. This study presents the development of NO-fibers, and the feasibility of NO-fibers to efficiently store and deliver NO, which can be further developed as a bandage.

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