scholarly journals The Sperm Structure and Spermatogenesis of Trypophloeus klimeschi (Coleoptera: Curculionidae: Scolytinae)

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 583
Author(s):  
Jing Gao ◽  
Guanqun Gao ◽  
Jiaxing Wang ◽  
Hui Chen
Keyword(s):  
Electron Microscopy ◽  
Golgi Apparatus ◽  
Reproductive System ◽  
Chromatin Condensation ◽  
Ejaculatory Duct ◽  
Seminal Vesicles ◽  
Male Reproductive System ◽  
Nuclear Chromatin ◽  
Accessory Glands ◽  
Close Phylogenetic Relationship

The male reproductive system, sperm structure, and spermatogenesis of Trypophloeusklimeschi (Coleoptera: Curculionidae: Scolytinae), which is one of the most destructive pests of Populus alba var. pyramidalis (Bunge), were investigated using light microscopy, scanning electron microscopy, and transmission electron microscopy. The male reproductive system of T.klimeschi is composed of testes, seminal vesicles, tubular accessory glands, multilobulated accessory glands, vasa deferentia, and a common ejaculatory duct. In spermatogenesis, two phenomena are apparent: The nuclear chromatin condenses into two different patterns, and an oval preacrosomal vesicle is present at the flank of the Golgi apparatus. The sperm are short, measuring 76.7 ± 1.8 μm in length, and are 508.1 ± 12.9 nm in width. The sperm are composed of a three-layer acrosomal complex, a cylindrical nucleus, two mitochondrial derivatives, a 9 + 9 + 2 axoneme, and two accessory bodies with a large “puff”-like expansion. Mature sperm are individually stored in seminal vesicles. During spermiogenesis, the similarities in the nuclear chromatin condensation characteristics of Curculioninae and Scolytinae are indicative of their close phylogenetic relationship. It appears that the preacrosomal vesicle being flanked by the Golgi apparatus is a characteristic of spermatogenesis in Curculionidae.

scholarly journals The Concept of Male Reproductive Anatomy

2021 ◽  
Author(s):  
Oyovwi Mega Obukohwo ◽  
Nwangwa Eze Kingsley ◽  
Rotu Arientare Rume ◽  
Emojevwe Victor
Keyword(s):  
Reproductive System ◽  
Reproductive Tract ◽  
Prostate Gland ◽  
Ejaculatory Duct ◽  
Seminal Vesicles ◽  
Female Reproductive Tract ◽  
Male Reproductive System ◽  
Male Gametes ◽  
Human Reproductive ◽  
Reproductive Anatomy

The human reproductive system is made up of the primary and secondary organs, which helps to enhances reproduction. The male reproductive system is designed to produce male gametes and convey them to the female reproductive tract through the use of supportive fluids and testosterone synthesis. The paired testis (site of testosterone and sperm generation), scrotum (compartment for testis localisation), epididymis, vas deferens, seminal vesicles, prostate gland, bulbourethral gland, ejaculatory duct, urethra, and penis are the parts of the male reproductive system. The auxiliary organs aid in the maturation and transportation of sperm. Semen is made up of sperm and the secretions of the seminal vesicles, prostate, and bulbourethral glands (the ejaculate). Ejaculate is delivered to the female reproduc¬tive tract by the penis and urethra. The anatomy, embryology and functions of the male reproductive system are discussed in this chapter.

scholarly journals A Glycoproteinaceous Secretion in the Seminal Vesicles of the Termite Coptotermes gestroi (Isoptera: Rhinotermitidae)

Insects ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 428
Author(s):  
Lara T. Laranjo ◽  
Ives Haifig ◽  
Ana Maria Costa-Leonardo
Keyword(s):  
Reproductive System ◽  
Periodic Acid ◽  
First Record ◽  
Distal Portion ◽  
Seminal Vesicles ◽  
Male Reproductive System ◽  
Periodic Acid Schiff ◽  
Accessory Glands ◽  
Primary Reproductives ◽  
Coptotermes Gestroi

Coptotermes gestroi is a subterranean termite with colonies generally headed by a pair of primary reproductives, although neotenics may occur. In this study, the male reproductive system was compared during different life stages of nymphs, alates, neotenic reproductives, and kings of C. gestroi, focusing on the modifications of this system along the maturation of these individuals. The structure of the male reproductive system follows the pattern described for insects, although C. gestroi males do not exhibit conspicuous penises and differentiated accessory glands. In kings, each testis consisted of about seven lobes, significantly increased in size as compared to younger males. The spermatogenesis begins in third-instar nymphs, which already presented spermatozoa in the testes. The seminal vesicles are individualized in C. gestroi and have a secretory distal portion and a proximal portion with a role in spermatozoa storage. The secretion of the seminal vesicles is strongly periodic acid Schiff (PAS)-positive, whereas the xylidine Ponceau test revealed proteins that increase in quantity while the males become older. This is the first record of glycoproteins in the lumen of seminal vesicles in termites. Further studies will clarify how they are produced and interact in the physiology and nutrition of the non-flagellate spermatozoa of C. gestroi.

The systematic position of Chaitophorinae (Hemiptera, Aphidoidea) in the light of anatomy research

2004 ◽  
Vol 35 (3) ◽  
pp. 317-327 ◽  
Author(s):  
Waclaw Wojciechowski ◽  
Karina Wieczorek
Keyword(s):  
Cross Sections ◽  
Reproductive System ◽  
Ejaculatory Duct ◽  
Systematic Position ◽  
Male Reproductive System ◽  
Taxonomic Position ◽  
Morphological Data ◽  
Histological Structure ◽  
Accessory Glands ◽  
Paraffin Method

AbstractThe structure of the male reproductive system of 14 Chaitophorine species is discussed: Chaitophorus capreae, C. leucomelas, C. nassonovi, C. populeti, C. populialbae, C. salicti, C. salijaponicus niger, C. tremulae, C. truncatus, C. vittelinae, Sipha maydis, Periphyllus aceris, P. coracinus, P. lyropictus. All species, except Sipha maydis, which lives on Poacea (grasses), feed on trees of the families Aceraceae (maples) and Salicaceae (poplars and willows). On the basis of longitudinal sections, cross-sections (paraffin method) and total preparation, the following elements in the structure of the male reproductive system have been examined: the number of testis follicles and the stage of the spermatogenesis; the connection of follicles and vasa deferentia, the position and the histological structure of the wall of vasa deferentia; and the development accessory glands and ejaculatory duct. These characters were then used to supplement morphological data in order to verify the taxonomic position of Chaitophorinae.

scholarly journals Morphology and histology of the male reproductive system of Collaria oleosa (Distant, 1883) (Heteroptera: Miridae)

Bionatura ◽  
2020 ◽  
Vol 5 (2) ◽  
pp. 1122-1125
Author(s):  
Mayra Vélez ◽  
Glenda Dias ◽  
Irina Morales ◽  
Raul N. C. Guedes ◽  
José Lino-Neto
Keyword(s):  
Reproductive System ◽  
Vas Deferens ◽  
Seminal Vesicles ◽  
Male Reproductive System ◽  
General Morphology ◽  
Accessory Glands ◽  
New Information ◽  
Ejaculatory Bulb

Collaria oleosa (Distant, 1883) is a phytophagous bug often observed causing injuries in wheat, barley, and oat crops, besides grass pastures. The male reproductive system of C. oleosa has been studied anatomically and histologically. It consists of a pair of testes, each one with two follicles, one pair of seminal vesicles, two pairs of external accessory glands, and an ejaculatory bulb. The testicular follicles exhibit cysts with spermatogonia, spermatocytes, spermatids, and spermatozoa bundles. The testes are connected to the seminal vesicles by the vas deferens. The seminal vesicles are connected to the ejaculatory bulb and are bound by the accessory glands. In mirids belonging to the Stenodemini tribe, the general morphology of the male reproductive system is similar. However, two follicles per testis may be characteristic of the genus Collaria. The anatomy and histology of the male reproductive system in C. oleosa provides new information and also contributes to the understanding of the systematics of Stenodemini as well as the Miridae family in general.

Anatomical investigations of the male reproductive system of five species of Calaphidinae (Hemiptera, Aphidoidea)

2006 ◽  
Vol 37 (4) ◽  
pp. 457-465 ◽  
Author(s):  
Karina Wieczorek
Keyword(s):  
Reproductive System ◽  
Ejaculatory Duct ◽  
Proximal Part ◽  
Male Reproductive System ◽  
Morphological Data ◽  
Developmental Trends ◽  
Histological Sections ◽  
Accessory Glands ◽  
Whole Mount

AbstractThe structure of the male reproductive system of 5 species of Calaphidinae Clethrobius comes, Euceraphis betulae, Myzocallis (Lineomyzocallis) walshii, Tuberculatus (Tuberculoides) annulatus and Tuberculatus (Tuberculoides) neglectus is discussed. C. comes and E. betulae feed on birches (Betula), whereas M. (L.) walshii, T. (T.) annulatus, and T. (T.) neglectus are associated with oaks (Quercus). On the basis of histological sections and whole mount preparations the following elements in the structure of the male reproductive system have been examined: the number of testis follicles and the stage of spermatogenesis, the connection of follicles and vasa deferentia, the position of the proximal part of vasa deferentia as well as the development of accessory glands and the ejaculatory duct. In males of studied species the characters of the reproductive system have been observed: each testis holds 3 or 4 follicles, strongly elongated and arranged in a rosette. Vasa deferentia run separately along their length, only in the proximal part their walls cling together. Accessory glands are strongly elongated, sometimes asymmetric (T.(T.) annulatus) whereas the ejaculatory duct is usually reduced (in exception C. comes). These characters were then used to supplement morphological data in order to interpret developmental trends in the Calaphidinae.

Cellular analyses of sea urchin metamorphosis

Zygote ◽  
1999 ◽  
Vol 8 (S1) ◽  
pp. S77-S78
Author(s):  
Yukiko Sato ◽  
Ikuko Yazaki
Keyword(s):  
Electron Microscopy ◽  
Fatty Acids ◽  
Squamous Epithelium ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Chromatin Condensation ◽  
Environmental Cues ◽  
Nuclear Chromatin ◽  
Intracellular Changes ◽  
Hemicentrotus Pulcherrimus

Larvae of marine invertebrates undergo metamorphosis in response to environmental cues (Chia & Burke, 1978). In sea urchins, free fatty acids (Kitamura et al., 1993), dibromomethane (Taniguchi et al., 1994), pheromonal peptides (Burke, 1984) and L-glutamine (Yazaki & Harashima, 1994; Yazaki, 1995) have been known as metamorphosis-inducing substances. The mechanisms by which cells respond to these cues and how the larval tissues are absorbed have not been clear, however. In the present study, we used L-glutamine (Gln) and a natural cue, green algae (Ulvella sp.), to induce metamorphosis of Hemicentrotus pulcherrimus and Anthocidaris crassispina, and investigated the intracellular changes during metamorphosis.After being subjected to 10−5–10−3 M Gln for 10–24 h, larvae cease swimming, settle, begin to retract their larval arms, extrude the primary podia and finally evert their echinus rudiment (ER). In H. pulcherrimus, larvae retracted their arms from 6 h to 24 h after the start of Gln treatment and then everted the ER. A. crassispina larvae underwent similar processes to those of H. pulcherrimus. The larval surface is composed of squamous epithelium and columnar epithelium. The epithelium of the ciliary bands or epaulets is columnar.In the squamous epithelium, the nuclear chromatin in the larval arms and body, and in the oesophagus, markedly condensed after treatment with Gln for 24 h. Electron microscopy revealed swelling of both nuclei and mitochondria, while their membranes seemed to be intact. In the cytoplasm, lipid-like structures and electron-dense substances appeared. A further 24 h after Gln treatment, the chromatin condensation had progressed. Most nuclei in which chromatin had condensed were positive to the TUNEL assay, which detects DNA fragmentation. These results suggest that cell death in the squamous epithelium is apoptotic rather than necrotic.

scholarly journals Ovary Structure and Oogenesis of Trypophloeus klimeschi (Coleoptera: Curculionidae: Scolytinae)

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1099
Author(s):  
Jing Gao ◽  
Jiaxing Wang ◽  
Hui Chen
Keyword(s):  
Electron Microscopy ◽  
Reproductive System ◽  
Secretory Cells ◽  
Female Reproductive System ◽  
Transition Stage ◽  
Accessory Glands ◽  
Transmission Electron ◽  
Secondary Stage ◽  
Terminal Filament ◽  
Structure And Ultrastructure

The female reproductive system, ovary structure and ultrastructure of Trypophloeus klimeschi (Coleoptera: Curculionidae: Scolytinae) were investigated using light microscopy, scanning electron microscopy, and transmission electron microscopy. Its female reproductive system is comprised of two ovaries (each ovary has two ovarioles), lateral oviducts, common oviduct, spermathecal sac, spermathecal pump, two accessory glands and bursa copulatrix. Well-developed endoplasmic reticulum can be clearly seen in the secretory cells of spermathecal sac. This species has telotrophic meroistic ovarioles that are comprised of terminal filament, tropharium, vitellarium and pedicel. The terminal filaments are simple; each is comprised of cellular peritoneal sheath. The presence of several clusters of nurse cells in the tropharium is indicative that its ovarioles conform to the transition stage. This indicates that there are at least two different types (transition stage and secondary stage) of ovarioles in Curculionidae.

Ultrastructure of the epithelium of the upper ejaculatory duct of the mealworm beetle, Tenebrio molitor

1987 ◽  
Vol 45 ◽  
pp. 814-815
Author(s):  
S. Bricker ◽  
G. M. Happ
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ejaculatory Duct ◽  
Tenebrio Molitor ◽  
Shear Forces ◽  
Accessory Glands ◽  
Ultrastructural Evidence ◽  
Transmission Electron ◽  
Mealworm Beetle ◽  
Quinone Tanning

The male mealworm, Tenebrio molitor produces a spermatophore to facilitate transfer of sperm to the female. The wall of the spermatophore is largely produced from the secretions of the paired bean-shaped accessory glands (BAGs). As the cottony pre-spermatophoric mass from the BAGs comes together in the ejaculatory duct where it is molded into the spermatophore, it becomes tougher and more elastic. The mechanisms involved in this stabilization of the wall of the spermatophore were unknown. Mechanisms of stabilization of other acellular structures assembled in extracellular space include quinone-tanning and β-sclerotization in cuticle, shear forces in silk, and pH changes in the spermatophore of Rhodnius. The cells found in the epithelium of the upper ejaculatory duct of the mealworm beetle were examined by transmission electron microscopy for ultrastructural evidence of a role in the stabilization of the spermatophore wall.

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