scholarly journals Sperm Lipid Composition in Early Diverged Fish Species: Internal vs. External Mode of Fertilization

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 172 ◽  
Author(s):  
Kathrin M. Engel ◽  
Viktoriya Dzyuba ◽  
Alexandre Ninhaus-Silveira ◽  
Rosicleire Veríssimo-Silveira ◽  
Dirk Dannenberger ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Cholesterol Content ◽  
Internal Fertilization ◽  
External Fertilization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
External Mode ◽  
Adrenic Acid ◽  
Sperm Membrane ◽  
Layer Chromatography

The lipid composition of sperm membranes is crucial for fertilization and differs among species. As the evolution of internal fertilization modes in fishes is not understood, a comparative study of the sperm lipid composition in freshwater representatives of externally and internally fertilizing fishes is needed for a better understanding of taxa-specific relationships between the lipid composition of the sperm membrane and the sperm physiology. The lipidomes of spermatozoa from stingray, a representative of cartilaginous fishes possessing internal fertilization, and sterlet, a representative of chondrostean fishes with external fertilization, have been studied by means of nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray MS, gas chromatography-(GC) MS, and thin-layer chromatography (TLC). NMR experiments revealed higher cholesterol content and the presence of phosphatidylserine in stingray compared to sterlet sperm. Unknown MS signals could be assigned to different glycosphingolipids in sterlet (neutral glycosphingolipid Gal-Cer(d18:1/16:0)) and stingray (acidic glycosphingolipid sulpho-Gal-Cer(d18:1/16:0)). Free fatty acids in sterlet sperm indicate internal energy storage. GC-MS experiments indicated a significant amount of adrenic acid, but only a low amount of docosahexaenoic acid in stingray sperm. In a nutshell, this study provides novel data on sperm lipid composition for freshwater stingray and sterlet possessing different modes of fertilization.

scholarly journals Lipidoak aztertu eta identifikatzeko TLC kromatografiaren eta MALDI masa-espektrometriaren arteko akoplamendua

2018 ◽  
pp. 149-165
Author(s):  
Antonio Veloso
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Maldi Ims ◽  
Layer Chromatography ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Geruza meheko kromatografia teknika (TLC, ingelesezko Thin Layer Chromatography) oso erabilia izan da hainbat molekularen banaketa eta karakterizaziorako. Bestalde, Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) edo matrizez lagundutako laserraren bidezko desortzioionizazioa/hegaldi-denbora masa-espektrometria teknikaren erabilera gero eta gehiago hedatzen ari da molekula desberdinen analisia egiteko. Gainera, Imaging Mass Spectrometry (IMS) edo masa-espektrometriaren bidezko irudia izeneko teknikak, ahalbidetzen du ehun biologikoetan dauden lipidoen, proteinen eta farmakoen banaketa-irudiak lortzea eta teknika bikaina da molekula horien banaketa aztertzeko. Teknika honek lipido mota ezberdinen identifikazioa eta lokalizazioa ahalbidetzen du, konposatuaren edota familia kimikoaren aukera egin aurretik. Analizatu behar diren konposatuen aukera aldez aurretik egin behar ez denez, aukera dago irudiak sortzeko masa-espektrometroan detektatutako ioietatik abiatuta. Lan honetan, erakusten da lipidoen ikerkuntzarako oso lagungarria dela TLC xaflak eta MALDI IMS teknikak konbinatzea.

scholarly journals Identification and Characterization of the Genes Involved in Glycosylation Pathways of Mycobacterial Glycopeptidolipid Biosynthesis

2006 ◽  
Vol 188 (1) ◽  
pp. 86-95 ◽  
Author(s):  
Yuji Miyamoto ◽  
Tetsu Mukai ◽  
Noboru Nakata ◽  
Yumi Maeda ◽  
Masanori Kai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acyl ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
The Common ◽  
Glycosylation Pathway ◽  
Layer Chromatography ◽  
Identification And Characterization

ABSTRACT Glycopeptidolipids (GPLs) are major components present on the outer layers of the cell walls of several nontuberculous mycobacteria. GPLs are antigenic molecules and have variant oligosaccharides in mycobacteria such as Mycobacterium avium. In this study, we identified four genes (gtf1, gtf2, gtf3, and gtf4) in the genome of Mycobacterium smegmatis. These genes were independently inactivated by homologous recombination in M. smegmatis, and the structures of GPLs from each gene disruptant were analyzed. Thin-layer chromatography, gas chromatography-mass spectrometry, and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses revealed that the mutants Δgtf1 and Δgtf2 accumulated the fatty acyl-tetrapeptide core having O-methyl-rhamnose and 6-deoxy-talose as sugar residues, respectively. The mutant Δgtf4 possessed the same GPLs as the wild type, whereas the mutant Δgtf3 lacked two minor GPLs, consisting of 3-O-methyl-rhamnose attached to O-methyl-rhamnose of the fatty acyl-tetrapeptide core. These results indicate that the gtf1 and gtf2 genes are responsible for the early glycosylation steps of GPL biosynthesis and the gtf3 gene is involved in transferring a rhamnose residue not to 6-deoxy-talose but to an O-methyl-rhamnose residue. Moreover, a complementation experiment showed that M. avium gtfA and gtfB, which are deduced glycosyltransferase genes of GPL biosynthesis, restore complete GPL production in the mutants Δgtf1 and Δgtf2, respectively. Our findings propose that both M. smegmatis and M. avium have the common glycosylation pathway in the early steps of GPL biosynthesis but differ at the later stages.

Investigation of age, sex and menstrual stage variation in human cerumen lipid composition by high performance thin layer chromatography

2007 ◽  
Vol 122 (9) ◽  
pp. 881-886 ◽  
Author(s):  
M Koçer ◽  
T Güldür ◽  
M Akarçay ◽  
M C Miman ◽  
G Beker
Keyword(s):  
Menstrual Cycle ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lipid Composition ◽  
High Performance ◽  
Age Groups ◽  
Cholesterol Content ◽  
Protective Role ◽  
Wax Ester ◽  
Layer Chromatography

AbstractObjective:The objective of the study was to correlate quantitative changes in the lipid composition of human cerumen with changes in age, sex and menstrual cycle stage.Design:Cerumen samples were collected from the external ear canal and analysed using sequential, one dimensional, high performance thin layer chromatography.Subjects:The following age groups of both sexes were investigated: one to 10 years; 11–18 years; 19–40 years; and 40 years and over. Additionally, cerumen samples from subjects in three stages of the menstrual cycle were compared.Results:In the cerumen samples, the peak values for wax ester and cholesterol occured between the ages of one and 10 years for both sexes. However, squalene and triglyceride content reached maximum levels at puberty. Men aged 19–40 years had a significantly greater percentage of cerumen lipid squalene content than women from the same age group; however, their cholesterol content was found to be lower. Regarding the various menstrual cycle stages, cerumen samples taken at the follicular stage from women aged 19–40 years had a significantly lower free fatty acids content, and higher cholesterol and squalene levels, compared with samples taken in the luteal or menstrual stages.Conclusion:The proportions of the lipid constituents of cerumen varied with age, sex and menstrual stage. In cerumen, the main lipid constituent stimulated at puberty appears to be squalene, not wax esters as reported for sebum. The relevance of lipid constituents to cerumen's protective role is discussed.

scholarly journals Dopamine and iron mediated fragmentation of galactocerebroside and cardiolipin in micelles

2007 ◽  
Vol 5 (4) ◽  
Author(s):  
Irina Yurkova ◽  
Mikhail Kisel ◽  
Juergen Arnhold ◽  
Oleg Shadyro
Keyword(s):  
Mixed Micelles ◽  
Laser Desorption ◽  
Oh Radical ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Layer Chromatography ◽  
Radical Transformation ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

AbstractMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and thin-layer chromatography (TLC) have been used to study dopamine and iron mediated free-radical transformation of lipids in their hydrophilic parts. It has been shown that the action of the dopamine/Fe2+ system on galactocerebroside or cardiolipin, which are the components of mixed micelles, results in formation of ceramide or phosphatidic acid and phosphatidylhydroxyacetone, respectively. These data, when combined with results obtained using the ascorbate/Fe2+/H2O2 oxidizing system with the same substrates, demonstrate that the formation of these products proceeds via an OH-radical induced fragmentation taking place in polar moiety of the starting lipids.

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