scholarly journals Assessment of Fibrinogen Macromolecules Interaction with Red Blood Cells Membrane by Means of Laser Aggregometry, Flow Cytometry, and Optical Tweezers Combined with Microfluidics

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1448
Author(s):  
Alexey N. Semenov ◽  
Andrei E. Lugovtsov ◽  
Evgeny A. Shirshin ◽  
Boris P. Yakimov ◽  
Petr B. Ermolinskiy ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Significant Risk ◽  
In Vitro Study ◽  
Significant Risk Factor ◽  
Elevated Concentration ◽  
Rbc Membrane ◽  
Rbc Aggregation

An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase in red blood cells (RBC) aggregation, resulting in hemorheological disorders. Despite the biomedical importance, the mechanisms of fibrinogen-induced RBC aggregation are still debatable. One of the discussed models is the non-specific adsorption of fibrinogen macromolecules onto the RBC membrane, leading to the cells bridging in aggregates. However, recent works point to the specific character of the interaction between fibrinogen and the RBC membrane. Fibrinogen is the major physiological ligand of glycoproteins receptors IIbIIIa (GPIIbIIIa or αIIββ3 or CD41/CD61). Inhibitors of GPIIbIIIa are widely used in clinics for the treatment of various cardiovascular diseases as antiplatelets agents preventing the platelets’ aggregation. However, the effects of GPIIbIIIa inhibition on RBC aggregation are not sufficiently well studied. The objective of the present work was the complex multimodal in vitro study of the interaction between fibrinogen and the RBC membrane, revealing the role of GPIIbIIIa in the specificity of binding of fibrinogen by the RBC membrane and its involvement in the cells’ aggregation process. We demonstrate that GPIIbIIIa inhibition leads to a significant decrease in the adsorption of fibrinogen macromolecules onto the membrane, resulting in the reduction of RBC aggregation. We show that the mechanisms underlying these effects are governed by a decrease in the bridging components of RBC aggregation forces.

scholarly journals Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Micromachines ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 19 ◽  
Author(s):  
Tatiana Avsievich ◽  
Yana Tarakanchikova ◽  
Ruixue Zhu ◽  
Alexey Popov ◽  
Alexander Bykov ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Morphological Changes ◽  
Human Mesenchymal Stem Cells ◽  
Mutual Interaction ◽  
Combined Application ◽  
Rbc Membrane ◽  
Rbc Aggregation ◽  
Cross Bridges

In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

Abstract TP35: Estimation of Red Blood Cells in the Thrombus Using MRI. A Phantom Study with Predetermined Thrombus Components

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Ana Paula Narata ◽  
Isabelle Filipiak ◽  
Richard Bibi ◽  
Jean Philippe Cottier ◽  
Kevin Janot
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Spin Echo ◽  
In Vitro Study ◽  
Fibrin Clot ◽  
Fluid Attenuated Inversion Recovery ◽  
Magnetic Resonance Imaging Mri ◽  
C 50 ◽  
D 20

Background and Purpose: Better understanding about thrombus composition seems necessary, as treatment of acute ischemic stroke (AIS) is focus on clot chemical dissolution and mechanical extraction. We propose to evaluate whether magnetic resonance imaging (MRI) can differentiate white from red clots and estimate red blood cells percentage (RBC%) using clots with predetermined components and an index based on MRI signal intensity (SI). Material and Methods: 5 clots (A=100% fibrin, B=80% RBC, C=50% RBC, D=20% RBC, E=unknown) were fixed in gelatin-manganese solution and studied by: high-resolution 3D T1-weighted (T1MPR), T2-weighted turbo spin echo (T2TSE), T2-weighted gradient echo (T2GE), susceptibility weighted (SWI), fluid-attenuated inversion recovery (FLAIR) and diffusion weighted imaging (DWI) with apparent diffusion coefficient (ADC). SI index was calculated with clot SI and gelatin SI. Statistical analysis compared RBC-clots to fibrin-clot SI index and the correlation of RBC% and SI index in each MRI sequence. Results: Each red clot was different from clot A except clot D in FLAIR. Correlation between clots SI index and RBC concentration were found in T1MPR (r=-0.84), SWI (r=-0.79), T2GE (r=-0.72) and FLAIR (r=0.80). Linear regression resolution provided an indirect RBC estimation for clot E: 47.3 % in T1MPR, SWI 41.5%, T2GE 45.1% and FLAIR 50.9%. Histological analysis confirmed clot E composition. Conclusion: This in vitro study suggests that MRI can differentiate white from red clots except clots with low RBC% in FLAIR and also provide approximate RBC%.

scholarly journals An In Vitro Study of the Effect of Cytotoxic Triorganotin Dimethylaminophenylazobenzoate Complexes on Red Blood Cells

2018 ◽  
Vol 251 (5-6) ◽  
pp. 735-745 ◽  
Author(s):  
Hanna Pruchnik ◽  
Aleksandra Włoch ◽  
Dorota Bonarska-Kujawa ◽  
Halina Kleszczyńska
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

In vitro study on the partitioning of red blood cells using a microchannel network

2022 ◽  
Vol 140 ◽  
pp. 104281
Author(s):  
Toru Hyakutake ◽  
Hiroki Abe ◽  
Yohei Miyoshi ◽  
Manabu Yasui ◽  
Rina Suzuki ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Evaluation of chromium in red blood cells as an indicator of exposure to hexavalent chromium: An in vitro study

2016 ◽  
Vol 255 ◽  
pp. 63-70 ◽  
Author(s):  
Jérôme Devoy ◽  
Antoine Géhin ◽  
Samuel Müller ◽  
Mathieu Melczer ◽  
Aurélie Remy ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Hexavalent Chromium ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

In vitro study of the specific interaction between poly(2-dimethylamino ethylmethacrylate) based polymers with platelets and red blood cells

2015 ◽  
Vol 492 (1-2) ◽  
pp. 55-64 ◽  
Author(s):  
Luca Flebus ◽  
François Lombart ◽  
Lucía Martinez-Jothar ◽  
Chantal Sevrin ◽  
Céline Delierneux ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Specific Interaction ◽  
In Vitro Study ◽  
Vitro Study

Influence of plasma preparations and donor red blood cells on the antioxidant capacity of blood from newborn babies: an in vitro study

1996 ◽  
Vol 85 (2) ◽  
pp. 220-224 ◽  
Author(s):  
RMW Moison ◽  
EJHA Hoof ◽  
PC Clahsen ◽  
D Zoeren-Grobben ◽  
HM Berger
Keyword(s):  
Antioxidant Capacity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals In vitro effect of pentoxifylline and lisofylline on deformability and aggregation of red blood cells from healthy subjects and patients with chronic venous disease.

2013 ◽  
Vol 60 (1) ◽  
Author(s):  
Karolina Słoczyńska ◽  
Mariusz Kózka ◽  
Elżbieta Pękala ◽  
Anna Marchewka ◽  
Henryk Marona
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Chronic Venous Disease ◽  
Venous Disease ◽  
Active Metabolites ◽  
Antecubital Vein ◽  
Rbc Aggregation ◽  
Vitro Effect

Purpose. The aim of the study was to assess the in vitro potency of pentoxifylline (PTX) and one of its most active metabolites lisofylline (LSF) to improve rheological properties of red blood cells (RBC) from healthy individuals and patients with chronic venous disease (CVD). Additionally, the study aimed to compare the effects of PTX and LSF on RBC deformability and aggregation. Methods. Blood samples were collected from healthy volunteers (antecubital vein) and from CVD patients (varicose and antecubital vein). Deformability and aggregation of RBC were assessed using Laser-assisted Optical Rotational Cell Analyser (LORCA). Results. PTX and LSF increased RBC elongation significantly. Additionally, RBC incubation with PTX resulted in a marked decrease in RBC aggregation. PTX reduced the tendency towards the formation of RBC aggregates and of their stability. The beneficial effect of PTX on RBC aggregation was most apparent for those cells whose aggregation tendency and aggregate stability was the greatest. Conclusions. In vitro addition of PTX or LSF effectively increased deformability of RBC from healthy donors and patients with CVD. Thus, LSF may contribute to the in vivo hemorheological effects of pentoxifylline. On the other hand, there was no significant effect of LSF on aggregation of RBC in vitro. Hence, LSF has no contribution to this particular effect of PTX. Additionally, the present study demonstrated the use of RBC with impaired deformability and aggregation for the evaluation of in vitro rheological activity of xenobiotics.

Melatonin chelates iron and binds directly with phenylhydrazine to provide protection against phenylhydrazine induced oxidative damage in red blood cells along with its antioxidant mechanisms: an in vitro study

2018 ◽  
Vol 1 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Sudeshna Paul ◽  
Shamreen Naaz ◽  
Arnab Kumar Ghosh ◽  
Sanatan Mishra ◽  
Aindrila Chattopadhyay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Molecular Mechanisms ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Causative Factor ◽  
Protective Effects

Oxidative stress is an important causative factor for a number of diseases. Phenylhydrazine (PHZ) is a widely accepted model for studying hemolytic anemia by induction of oxidative stress. In the present study, goat red blood cells (RBCs) were incubated in vitro with PHZ (1mM) to generate oxidative stress. To test whether melatonin exhibits protective effects on PHZ induced RBC damage and to explore its potential molecular mechanisms, different concentrations of melatonin (5, 10, 20 and 40 nmoles/ml) were also included. PHZ caused altered profiles on biomarkers of oxidative stress and antioxidative as well as glucose metabolic enzymes in RBCs. These alterations indicated a development of oxidative stress. Melatonin at a concentration of 40 nmoles/ml provided optimal protection against all alterations induced by PHZ. The important cellular membrane proteins, including spectrin and actin, were also damaged by PHZ and this led to RBC deformation similar to that of observed in severe β-thalassaemia; the RBC deformation was also prevented by melatonin. Binding profiles of melatonin with PHZ and ferrous iron indicated favorable binding of melatonin with both of them, respectively. Thus, in addition to the direct antioxidant and free radical scavenging capability, melatonin also inhibited iron overloading by chelating iron and binding with the PHZ. This action of melatonin further reduces free radical generation. Based on the results, melatonin may provide therapeutic relevance to ß-thalassemia and other hemolytic RBC disorders involving oxidative stress. 

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