Comparative Transcriptomic Analysis Reveals the Effects of Drought on the Biosynthesis of Methyleugenol in Asarum sieboldii Miq.

Asarum sieboldii Miq., a perennial herb in the family Aristolochiaceae, is widely used to treat colds, fever, headache and toothache in China. However, little is known about the drought-tolerance characteristics of A. sieboldii. In this study, to elucidate the molecular–genetic mechanisms of drought-stress tolerance of A. sieboldii, RNA-seq was conducted. In total, 53,344 unigenes were assembled, and 28,715 unigenes were annotated. A total of 6444 differential-expression unigenes (DEGs) were found, which were mainly enriched in phenylpropanoid, starch and sucrose metabolic pathways. Drought stress revealed significant up-regulation of the unigenes encoding PAL, C4H, HCT, C3H, CCR and IGS in the methyleugenol-biosynthesis pathway. Under the condition of maintaining drought for 15 days and 30 days, drought stress reduced the biosynthesis of volatile oil by 24% and 38%, respectively, while the production of key medicinal ingredients (such as methyl eugenol) was increased. These results provide valuable information about the diverse mechanisms of drought resistance in the A. sieboldii, and the changes in the expression of the genes involved in methyleugenol biosynthesis in response to drought stress.

Download Full-text

Drought tolerance molecular responses of two cultivated varieties Jerusalem artichoke (Helianthus tuberosus L.) as revealed by RNA-Seq

10.21203/rs.3.rs-124586/v1 ◽

2020 ◽

Author(s):

Shipeng Yang ◽

Lihui Wang ◽

Qiwen Zhong ◽

Guangnan Zhang ◽

Haiwang Zhang ◽

...

Keyword(s):

Drought Stress ◽

Drought Resistance ◽

Metabolic Pathways ◽

Jerusalem Artichoke ◽

Tibet Plateau ◽

Helianthus Tuberosus ◽

Rna Seq ◽

Concentration Gradients ◽

Light Reaction ◽

Metabolic Activities

Abstract Background Jerusalem artichoke (Helianthus tuberosus L.) is a highly stress-resistant crop, especially it grows normally in the desertified land of Qinghai-Tibet Plateau in the past two years, and has become a crop with agricultural, industrial and ecological functions. However, there are few studies on drought resistance of Jerusalem artichoke at present, and studies on the mechanisms of stress resistance of Jerusalem artichoke breeding and fructan are seriously lagging behind. In this study, we selected two differentially resistant cultivars for drought stress experiments with different concentration gradients, the aim was finding DEGs and metabolic pathways associated with drought stress. Results Based on an additional analysis of the metabolic pathways under drought stress using MapMan, the most different types of metabolism included secondary metabolism, light reaction metabolism and cell wall. As a whole, QY1 and QY3 both had a large number of up-regulated genes in the flavor pathway. It was suggested that flavonoids could help Jerusalem artichoke to resist drought stress and maintain normal metabolic activities. In addition, the gene analysis of the abscisic acid (ABA) key metabolic pathway showed that QY3 had more genes in NAC and WRKY than QY1, but QY1 had more genes in response to drought stress as a whole. By combining RNA-Seq and WGCNA, a weighted gene co-expression network was constructed and divided into modules. By analyzing specifically the expressed modules, four modules were found to have the highest correlation with drought. Further research on the genes revealed that all 16 genes related to histone, ABA and protein kinase had the highest significance in these pathways. Conclusions These findings represent the first RNA-Seq analysis of drought stress in Jerusalem artichoke, which is of substantial significance to explore the function of drought resistance in Jerusalem artichoke and the excavation of related genes.

Download Full-text

Comparative transcriptomic analysis of contrasting hybrid cultivars reveal key drought-responsive genes and metabolic pathways regulating drought stress tolerance in maize at various stages

PLoS ONE ◽

10.1371/journal.pone.0240468 ◽

2020 ◽

Vol 15 (10) ◽

pp. e0240468

Author(s):

Songtao Liu ◽

Tinashe Zenda ◽

Jiao Li ◽

Yafei Wang ◽

Xinyue Liu ◽

...

Keyword(s):

Drought Stress ◽

Stress Tolerance ◽

Metabolic Pathways ◽

Transcriptomic Analysis ◽

Drought Stress Tolerance ◽

Comparative Transcriptomic Analysis

Download Full-text

Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

Scientific Reports ◽

10.1038/s41598-021-89350-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Qingyuan Li ◽

Xiaojun Su ◽

Huanhuan Ma ◽

Kebing Du ◽

Min Yang ◽

...

Keyword(s):

Genetic Relationships ◽

Molecular Genetic ◽

Geographic Origin ◽

Rna Seq ◽

Camellia Japonica ◽

The Past ◽

Genic Ssr ◽

The Family ◽

Simple Sequence ◽

Molecular Makers

AbstractCamellia is a genus of flowering plants in the family Theaceae, and several species in this genus have economic importance. Although a great deal of molecular makers has been developed for molecular assisted breeding in genus Camellia in the past decade, the number of simple sequence repeats (SSRs) publicly available for plants in this genus is insufficient. In this study, a total of 28,854 potential SSRs were identified with a frequency of 4.63 kb. A total of 172 primer pairs were synthesized and preliminarily screened in 10 C. japonica accessions, and of these primer pairs, 111 were found to be polymorphic. Fifty-one polymorphic SSR markers were randomly selected to perform further analysis of the genetic relationships of 89 accessions across the genus Camellia. Cluster analysis revealed major clusters corresponding to those based on taxonomic classification and geographic origin. Furthermore, all the genotypes of C. japonica separated and consistently grouped well in the genetic structure analysis. The results of the present study provide high-quality SSR resources for molecular genetic breeding studies in camellia plants.

Download Full-text

Organization of metabolic pathways and molecular-genetic mechanisms of xenobiotic degradation in microorganisms: A review

Applied Biochemistry and Microbiology ◽

10.1134/s0003683808020014 ◽

2008 ◽

Vol 44 (2) ◽

pp. 117-135 ◽

Cited By ~ 24

Author(s):

V. G. Khomenkov ◽

A. B. Shevelev ◽

V. G. Zhukov ◽

N. A. Zagustina ◽

A. M. Bezborodov ◽

...

Keyword(s):

Metabolic Pathways ◽

Molecular Genetic ◽

Genetic Mechanisms ◽

Xenobiotic Degradation

Download Full-text

Transcriptome analysis of oil palm (Elaeis guineensis Jacq.) roots under waterlogging stress

Plant Omics ◽

10.21475/poj.13.01.20.p2327 ◽

2020 ◽

pp. 46-56

Author(s):

Sunya Nuanlaong ◽

Suwit Wuthisuthimethavee ◽

Maruay Mekanawakul ◽

Potjamarn Suraninpong

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Wide Spectrum ◽

Molecular Genetic ◽

Ion Torrent ◽

Rna Seq ◽

Waterlogging Stress ◽

Physiological Processes ◽

Genetic Mechanisms ◽

Ion Torrent Sequencing

Waterlogging seriously constrains growth and yields in oil palm. To date, the responsive molecular changes caused by waterlogging in oil palm remain elusive. To elucidate the molecular genetic mechanisms of waterlogging stress, two varieties of oil palm Deli x Lamé and Deli x Ghana were used. The transcriptome profiles of the roots under waterlogging stress and normal conditions were compared via Ion Torrent Sequencing. Four libraries (GNR, GSR, SNR, and SSR) of oil palm roots after 45 days of normal watering and waterlogging stress were constructed. Approximately 6.2 million sequenced reads per library were obtained, with 5.5 million mapped reads (88.64%) similar to the oil palm genome in the GenBank database. A comparison of GNR/GSR showed a high of 3,289 DEGs with most genes up-regulated (1,863 DEGs). The GO analysis revealed the distribution of the DEGs among various pathways, suggesting a wide spectrum of physiological processes impacted by waterlogging stress. Moreover, qRT-PCR showed strong expression of all selected RNA-seq genes in waterlogged Deli x Ghana (GSR), especially GST, SAPK10 and NAC29 that are reported for the time to respond to waterlogging stress. Thus, this study not only reveals the comprehensive mechanisms of waterlogging responsive transcription in oil palm, but also establishes Deli x Ghana as a highly-adaptable variety to waterlogging conditions.

Download Full-text

Investigation on Various Aboveground Traits to Identify Drought Tolerance in Cowpea Seedlings

HortScience ◽

10.21273/hortsci13278-18 ◽

2018 ◽

Vol 53 (12) ◽

pp. 1757-1765 ◽

Cited By ~ 2

Author(s):

Waltram Ravelombola ◽

Ainong Shi ◽

Jun Qin ◽

Yuejin Weng ◽

Gehendra Bhattarai ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Vegetative Growth ◽

Root Architecture ◽

Crop Production ◽

Plant Morphology ◽

Delayed Senescence ◽

Cowpea Cultivars ◽

Genetic Mechanisms ◽

Effects Of Drought

Impacts of drought stress on crop production can significantly impair farmer’s revenue, hence adversely impacting the gross national product growth. For cowpea [Vigna unguiculata (L.) Walp.], which is a legume of economic importance, effects of drought at early vegetative growth could lead to substantial yield losses. However, little has been done with respect to breeding for cowpea cultivars withstanding drought at early vegetative growth. In addition, previous investigations have focused on how plant morphology and root architecture can confer drought tolerance in cowpea, which is not sufficient in efforts to unravel unknown drought tolerance–related genetic mechanisms, potentially of great importance in breeding, and not pertaining to either plant morphology or root architecture. Therefore, the objective of this study was to evaluate aboveground drought-related traits of cowpea genotypes at seedling stage. A total of 30 cowpea genotypes were greenhouse grown within boxes and the experimental design was completely randomized with three replicates. Drought stress was imposed for 28 days. Data on a total of 17 aboveground-related traits were collected. Results showed the following: 1) a large variation in these traits was found among the genotypes; 2) more trifoliate wilt/chlorosis tolerance but more unifoliate wilt/chlorosis susceptible were observed; 3) delayed senescence was related to the ability of maintaining a balanced chlorophyll content in both unifoliate and trifoliate leaves; and 4) the genotypes PI293469, PI349674, and PI293568 were found to be slow wilting and drought tolerant. These results could contribute to advancing breeding programs for drought tolerance in cowpea.

Download Full-text