Examination of Oral Squamous Cell Carcinoma and Precancerous Lesions Using Proximity Extension Assay and Salivary RNA Quantification

Saliva is an easy-to access body fluid with high diagnostic potential. The utilization of saliva for oral cancer diagnosis can be an attractive possibility. Besides the oral cancer, it is important to better understand the precancerous lesions such as oral lichen planus (OLP) and leukoplakia (OLK). In order to examine the changes of salivary proteins in controls, patients with oral cancer, and patients with precancerous conditions, proximity extension assay was utilized. Some proteins and functions were characteristic to the examined groups and can serve as a starting point for further biomarker studies. The different nature of OLK and OLP was demonstrated, showing the malignant transformation and the inflammation as the prominent biological processes in the OLK and OLP, respectively. The salivary level of IL6 was verified using quantitative ELISA and the mRNA level was also studied. Elevated IL6 levels could be detected in precancerous groups compared to controls.

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Complete Blood Count as a Diagnostic Marker in Oral Lichen Planus

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl3.3510 ◽

2020 ◽

Vol 11 (SPL3) ◽

pp. 1766-1771

Author(s):

Indra G ◽

Maragathavalli G ◽

Deepika Rajendran

Keyword(s):

Oral Cancer ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Blood Count ◽

Diagnostic Marker ◽

Complete Blood Count ◽

Precancerous Lesions ◽

Oral Submucous Fibrosis ◽

Oral Precancerous Lesions ◽

Oral Lichen

Oral precancerous lesions and conditions like oral lichen planus, leukoplakia, or oral submucous fibrosis have the potential to develop into oral cancer. Oral cancer accounts for approximately 3 % of all malignancies. Oral lichen planus is a premalignant condition occurring in the oral cavity due to various factors. Blood investigations are routinely done minimally invasive procedures carried out for various diagnostic purposes. The study aims to identify if the complete blood count (CBC) can be used as a pathologic diagnostic marker in an oral premalignant disorder like oral lichen planus. A total of 64 patients (38 female, 26 male) were taken into the study between the period July 2019 - March 2020. Each patient is exposed to blood investigations, and their results are studied. The results of the study show a minimal variation in the blood results. In conclusion, to determine if it can be used as a pathological diagnostic marker in premalignant disorders like oral lichen planus, further studies must be carried out in larger populations.

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Salivary endothelin-1 potential for detecting oral cancer in patients with oral lichen planus or oral cancer in remission

Oral Oncology ◽

10.1016/j.oraloncology.2011.07.032 ◽

2011 ◽

Vol 47 (12) ◽

pp. 1122-1126 ◽

Cited By ~ 35

Author(s):

Yi-Shing Lisa Cheng ◽

Terry Rees ◽

Lee Jordan ◽

Lance Oxford ◽

John O’Brien ◽

...

Keyword(s):

Oral Cancer ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Endothelin 1 ◽

Oral Lichen

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CD146 expression in oral lichen planus and oral cancer

Clinical Oral Investigations ◽

10.1007/s00784-019-02871-7 ◽

2019 ◽

Vol 24 (1) ◽

pp. 325-332 ◽

Cited By ~ 1

Author(s):

Sarinthon Pariyawathee ◽

Ekarat Phattarataratip ◽

Kobkan Thongprasom

Keyword(s):

Oral Cancer ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Oral Lichen ◽

Cd146 Expression

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Oral lichen planus (OLP), oral lichenoid lesions (OLL), oral dysplasia, and oral cancer: retrospective analysis of clinicopathological data from 2002–2011

Oral and Maxillofacial Surgery ◽

10.1007/s10006-014-0469-y ◽

2014 ◽

Vol 19 (2) ◽

pp. 149-156 ◽

Cited By ~ 25

Author(s):

S. Casparis ◽

J. M. Borm ◽

S. Tektas ◽

J. Kamarachev ◽

M. C. Locher ◽

...

Keyword(s):

Oral Cancer ◽

Retrospective Analysis ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Oral Dysplasia ◽

Oral Lichenoid Lesions ◽

Oral Lichen

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A clinical study on precancerous lesions of oral cavity in patients attending dental hospital, Telangana

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20195048 ◽

2019 ◽

Vol 6 (11) ◽

pp. 4739

Author(s):

Soundarya Hanumakonda ◽

Marri Swetha ◽

Mallela Bhashitha ◽

Harsha Mudigonda

Keyword(s):

Oral Cavity ◽

Oral Lichen Planus ◽

Precancerous Lesions ◽

Oral Submucous Fibrosis ◽

Age Distribution ◽

Precancerous Lesion ◽

Number Of Patients ◽

Dental Hospital ◽

Submucous Fibrosis ◽

Oral Lichen

Background: Aim of the retrospective study is to determine the type and frequency of the precancerous lesions of the oral cavity and to assess their sex, age distribution among the patients.Methods: Study was conducted based on year-wise data collected during 2015 to 2018 of all the patients with precancerous lesions of oral cavity.Results: The total number of patients was 1247, out of which 803 (64.39%) were males and 444 (35.60%) were females. Most common precancerous lesion is oral submucous fibrosis (70.24%) followed by oral lichen planus (19.08%), leukoplakia (8.98%), erythroplakia (1.68%).Conclusions: We observed marked geographical variation in the incidences of various precancerous lesions. Mostly males are affected by precancerous lesions than females.

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Detection of hepatitis C virus RNA in oral lichen planus and oral cancer tissues

Journal of Oral Pathology and Medicine ◽

10.1034/j.1600-0714.2000.290604.x ◽

2000 ◽

Vol 29 (6) ◽

pp. 259-266 ◽

Cited By ~ 75

Author(s):

Yumiko Nagao ◽

Michio Sata ◽

Seiji Noguchi ◽

Tamiko Seno'O ◽

Moritoshi Kinoshita ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Oral Cancer ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Hepatitis C Virus Rna ◽

Virus Rna ◽

Cancer Tissues ◽

Oral Lichen

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Assessment and Comparison of Salivary Survivin Biomarker in Oral Leukoplakia, Oral Lichen Planus, and Oral Cancer: A Comparative Study

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1415 ◽

2017 ◽

Vol 8 (2) ◽

pp. 73-76 ◽

Cited By ~ 1

Author(s):

Ruchika Garg ◽

Arvind V Shetti ◽

Anjana S Bagewadi

Keyword(s):

Oral Cancer ◽

Comparative Study ◽

Malignant Transformation ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Oral Leukoplakia ◽

Control Group ◽

Significant Difference ◽

Potentially Malignant ◽

Oral Lichen

ABSTRACT Introduction Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity. It is preceded by various potentially malignant conditions like oral leukoplakia and oral lichen planus. Survivin is an inhibitor of apoptosis whose levels have been found increased in oral cancer as well as various potentially malignant conditions. Thus survivin can act as a biomarker and help in early detection of potentially malignant conditions which can prevent its transformation into malignancy. Aim To assess and compare the level of total human salivary survivin in oral leukoplakia, oral lichen planus, oral cancer, and control group. Materials and methods A total of 96 subjects were included in the study, which were further grouped into 24 in each group. The saliva was analyzed for survivin level among all the four groups. Survivin concentration (pg/mL) was studied in relation to clinical data. The results were analyzed using Mann—Whitney U test to derive the statistical difference. Results The average of levels of survivin in control group was 0.199 pg/mL, in oral leukoplakia group 0.312 pg/mL, in oral lichen planus group 0.380 pg/mL, and in oral cancer group 0.430 pg/mL. A comparison of all these groups revealed statistically significant difference among the groups. Conclusion Survivin may not be considered as an independent predictor of the malignant transformation for premalignant lesions but it can be an indicator for an increased risk of malignant transformation. How to cite this article Garg R, Shetti AV, Bagewadi AS. Assessment and Comparison of Salivary Survivin Biomarker in Oral Leukoplakia, Oral Lichen Planus, and Oral Cancer: A Comparative Study. World J Dent 2017;8(2):73-76.

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