scholarly journals Cortisol Dose-Dependently Impairs Migration and Tube-like Formation in a Trophoblast Cell Line and Modulates Inflammatory and Angiogenic Genes

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 980
Author(s):  
Talita Guerreiro Rodrigues Húngaro ◽  
Marcos F. Gregnani ◽  
Thaís Alves-Silva ◽  
Florian Herse ◽  
Natalia Alenina ◽  
...  
Keyword(s):  
Cell Line ◽  
Placental Tissue ◽  
Trophoblast Cell ◽  
Continuous Treatment ◽  
Extravillous Trophoblast ◽  
Continuous Exposure ◽  
Trophoblastic Cells ◽  
And Function ◽  
Angiogenic Genes ◽  
Trophoblast Cell Line

Several stimuli can change maternal hormone levels during pregnancy. These changes may affect trophoblastic cells and modulate the development of the embryo and the placental tissue itself. Changes in cortisol levels are associated with impaired trophoblast implantation and function, in addition to other pregnancy complications. This study aims to analyze the effects of low and high doses of cortisol on an extravillous trophoblast cell line, and the effects of various exposures to this hormone. SGHPL-4 cells were treated with cortisol at five doses (0–1000 nM) and two exposures (continuous: 24 h/day; and intermittent: 2 h/day). In intermittent treatment, cortisol acted mainly as an anti-inflammatory hormone, repressing gene expression of kinin B1 receptors, interleukin-6, and interleukin-1β. Continuous treatment modulated inflammatory and angiogenic pathways, significantly repressing angiogenic factors and their receptors. Cortisol affected cell migration and tube-like structures formation. In conclusion, both continuous and intermittent exposure to cortisol repressed the expression of inflammatory genes, while only continuous exposure repressed the expression of angiogenic genes, suggesting that a sustained increase in the levels of this hormone is more harmful than a high short-term increase. Cortisol also impaired tube-like structures formation, and kinin receptors may be involved in this response.

scholarly journals Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Yi Wang ◽  
Xi-Ping Luo ◽  
Chi Eung Danforn Lim ◽  
Wu Shun Felix Wong ◽  
Gang Zhong
Keyword(s):  
Cell Line ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Regulatory Effect ◽  
Significant Difference ◽  
Mrna And Protein Expression ◽  
Toll Like Receptor 2 ◽  
Polymerase Chain ◽  
Tlr2 Mrna ◽  
Trophoblast Cell Line

Objective. To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1). Methods. TEV-1 cells were incubated with different doses of peptidoglycan. The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining. Results. TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane. Compared with the untreated control, TEV-1 cells incubated with 30 g/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment (). However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control () after 24 hours of treatment. On the other hand, 10 g/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (). Conclusion. Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells. It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.

scholarly journals Limited replication of human cytomegalovirus in a trophoblast cell line

2021 ◽  
Vol 102 (11) ◽  
Author(s):  
Kadeem Hyde ◽  
Nowshin Sultana ◽  
Andy C. Tran ◽  
Narina Bileckaja ◽  
Claire L. Donald ◽  
...  
Keyword(s):  
Cell Line ◽  
Vertical Transmission ◽  
Human Cytomegalovirus ◽  
Molecular Mechanisms ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Cell Protein ◽  
Trophoblast Cells ◽  
Hcmv Replication ◽  
Trophoblast Cell Line

Several viruses, including human cytomegalovirus (HCMV), are thought to replicate in the placenta. However, there is little understanding of the molecular mechanisms involved in HCMV replication in this tissue. We investigated replication of HCMV in the extravillous trophoblast cell line SGHPL-4, a commonly used model of HCMV replication in the placenta. We found limited HCMV protein expression and virus replication in SGHPL-4 cells. This was associated with a lack of trophoblast progenitor cell protein markers in SGHPL-4 cells, suggesting a relationship between trophoblast differentiation and limited HCMV replication. We proposed that limited HCMV replication in trophoblast cells is advantageous to vertical transmission of HCMV, as there is a greater opportunity for vertical transmission when the placenta is intact and functional. Furthermore, when we investigated the replication of other vertically transmitted viruses in SGHPL-4 cells we found some limitation to replication of Zika virus, but not herpes simplex virus. Thus, limited replication of some, but not all, vertically transmitted viruses may be a feature of trophoblast cells.

Endothelin-1 promotes migration and induces elevation of [Ca2+]i and phosphorylation of MAP kinase of a human extravillous trophoblast cell line

2003 ◽  
Vol 201 (1-2) ◽  
pp. 63-73 ◽  
Author(s):  
Chandan Chakraborty ◽  
Yousef P. Barbin ◽  
Subrata Chakrabarti ◽  
Peter Chidiac ◽  
S.Jeffrey Dixon ◽  
...  
Keyword(s):  
Map Kinase ◽  
Cell Line ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Endothelin 1 ◽  
Trophoblast Cell Line
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