scholarly journals MARCO+ Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1129
Author(s):  
Andrey Elchaninov ◽  
Anastasia Lokhonina ◽  
Polina Vishnyakova ◽  
Anna Soboleva ◽  
Anastasiya Poltavets ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Liver Resection ◽  
Liver Regeneration ◽  
Mouse Liver ◽  
Regenerating Liver ◽  
Male Mice ◽  
The Third ◽  
Macrophage Marker ◽  
Bone Marrow Origin ◽  
Collagenous Structure

Background: Macrophages play a key role in liver regeneration. The fates of resident macrophages after 70% resection are poorly investigated. In this work, using the MARCO macrophage marker (abbreviated from macrophage receptor with collagenous structure), we studied the dynamics of mouse liver resident macrophages after 70% resection. Methods: In BALB/c male mice, a model of liver regeneration after 70% resection was reproduced. The dynamics of markers CD68, TIM4, and MARCO were studied immunohistochemically and by using a Western blot. Results: The number of MARCO- and CD68-positive macrophages in the regenerating liver increased 1 day and 3 days after resection, respectively. At the same time, the content of the MARCO protein increased in the sorted macrophages of the regenerating liver on the third day. Conclusions: The data indicate that the number of MARCO-positive macrophages in the regenerating liver increases due to the activation of MARCO synthesis in the liver macrophages. The increased expression of MARCO by macrophages can be regarded as a sign of their activation. In the present study, stimulation with LPS led to an increase in the expression of the Marco gene in both Kupffer cells and macrophages of bone marrow origin.

scholarly journals Some Special Aspects of Liver Repair after Resection and Administration of Multipotent Stromal Cells in Experiment

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 66
Author(s):  
Igor Maiborodin ◽  
Elena Lushnikova ◽  
Marina Klinnikova ◽  
Swetlana Klochkova
Keyword(s):  
Bone Marrow ◽  
Liver Resection ◽  
Connective Tissue ◽  
Light Microscopy ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Rapid Restoration ◽  
Bone Marrow Origin

Changes in rat liver after resection and injection of autologous multipotent mesenchymal stromal cells of bone marrow origin (MSCs) transfected with the GFP gene and cell membranes stained with red-fluorescent lipophilic membrane dye were studied by light microscopy. It was found that after the introduction of MSCs into the damaged liver, their differentiation into any cells was not found. However, under the conditions of MSCs use, the number of neutrophils in the parenchyma normalizes earlier, and necrosis and hemorrhages disappear more quickly. It was concluded that the use of MSCs at liver resection for the rapid restoration of an organ is inappropriate, since the injected cells in vivo do not differentiate either into hepatocytes, into epithelial cells of bile capillaries, into endotheliocytes and pericytes of the vascular membranes, into fibroblasts of the scar or other connective tissue structures, or into any other cells present in the liver.

Interleukin-6 from intrahepatic cells of bone marrow origin is required for normal murine liver regeneration

Hepatology ◽  
2002 ◽  
Vol 35 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Xavier Aldeguer ◽  
Fotini Debonera ◽  
Abraham Shaked ◽  
Alyssa M. Krasinkas ◽  
Andrew E. Gelman ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Liver Regeneration ◽  
Interleukin 6 ◽  
Murine Liver ◽  
Bone Marrow Origin

scholarly journals A FINE STRUCTURE STUDY OF LIPID IN MOUSE LIVER REGENERATING AFTER PARTIAL HEPATECTOMY

1964 ◽  
Vol 21 (2) ◽  
pp. 233-244 ◽  
Author(s):  
Nancy L. Trotter
Keyword(s):  
Fine Structure ◽  
Partial Hepatectomy ◽  
Mouse Liver ◽  
Osmium Tetroxide ◽  
Structure Study ◽  
Regenerating Liver ◽  
Male Mice ◽  
Small Bodies ◽  
Cytoplasmic Bodies ◽  
Parenchymal Cells

The fine structure of liver 3½ to 72 hours after partial hepatectomy has been compared with that of liver from sham-operated animals; all animals were 60- to 90-day old male mice of the C3H strain. Numerous small bodies with diameters ranging from 300 to 1,000 A have been observed distributed randomly throughout the cytoplasm of the hepatic parenchymal cells at early intervals after partial hepatectomy. In material fixed in osmium tetroxide and embedded in methacrylate, they appear as uniformly electron-opaque bodies, but in permanganate-fixed liver, they display only a peripheral rim of electron-opaque material surrounding a clear core. Each of these cytoplasmic bodies appears to be located within a vesicle. A few of the opaque bodies are also present in sinusoids and in the spaces of Disse; these bodies are not located within vesicular structures. Fat droplets of various sizes are easily distinguished in regenerating liver; with the increase in number of these fat droplets at later postoperative intervals, there occurs a concomitant decrease in the number of cytoplasmic bodies. It is suggested that the cytoplasmic bodies contain some lipid component. Possible explanations of the origin, nature, and fate of the cytoplasmic bodies are discussed.

scholarly journals Modeling Dynamics and Function of Bone Marrow Cells in Mouse Liver Regeneration

Cell Reports ◽  
2017 ◽  
Vol 18 (1) ◽  
pp. 107-121 ◽  
Author(s):  
Elisa Pedone ◽  
Vlad-Aris Olteanu ◽  
Lucia Marucci ◽  
Maria Isabel Muñoz-Martin ◽  
Sameh A. Youssef ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Liver Regeneration ◽  
Mouse Liver ◽  
Bone Marrow Cells ◽  
Dynamics And Function ◽  
And Function ◽  
Marrow Cells

scholarly journals Pharmacological Mobilization of Endogenous Bone Marrow Stem Cells Promotes Liver Regeneration after Extensive Liver Resection in Rats

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Rujun Zhai ◽  
Yongchun Wang ◽  
Le Qi ◽  
George Melville Williams ◽  
Bin Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Resection ◽  
Liver Regeneration ◽  
Bone Marrow Stem Cells

scholarly journals Stimulation of the Liver Regeneration with Bone Marrow Mesenchymal Stem Cells

2021 ◽  
Vol 24 (5) ◽  
pp. 9-18
Author(s):  
Martina Vidová Uğurbaş ◽  
Jana Kaťuchová ◽  
Darina Petrášová ◽  
Timea Špaková ◽  
Jozef Radoňak
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Liver Resection ◽  
Liver Regeneration ◽  
Liver Tumors ◽  
Control Sample ◽  
Sprague Dawley ◽  
Extensive Resection ◽  
Stimulation Of

The main objective of our experiment was to prove the effect of mesenchymal stem cells of bone marrow (MSCs) on the stimulation of liver regeneration. The attention has been paid to adaptation of stem cells to the new environment and their transfer to anatomical structures. The experiment included 40 male Sprague Dawley (SD) rats aged 10 to 12 weeks. Biomodels were divided into five groups in the same number (n=8). Group 1 consisted of a control sample of eight healthy rats. Group 2 consisted of eight rats after liver resection without application of MSCs. Group 3 was after liver resection and application of MSCs. Group 4 after liver injury induce with Thioacetamide (TAA), without transplantation of MSCs. Group 5 was after chemical damage to the liver by TAA administration and MSCs transplantation. The process of stimulation of the liver was observed based on the laboratory values of alanine aminotransferase (ALT), albumin and bilirubin. The weight of the rats in each group was also compared. Animals were sacrificed after 1 day, 7 days, 14 days, and 21 days. In our experiment we found a statistically significant decrease in ALT (P≤0.001) and bilirubin (P≤0.001) was observed in the groups 3 and 5 (treated with MSCs) compared to the groups without MSCs (Groups 2 and 4). The increase in the albumin levels in the groups 3 and 5 was statistically significant. The results of our experiment led us to the conclusion, the transplantation of MSCs has important effect for the treatment and stimulation of liver regeneration following injury. MSCs administration may be extremely useful in a number of clinical applications in the treatment of liver tumors. It will allow us to perform extensive resection of the liver without risk of liver failure

Intrahepatic Portal Application of Autologous CD133+ Bone Marrow Stem Cells to the Liver - Early Experience with a Novel Concept To Support Hepatic Regeneration.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2338-2338
Author(s):  
Jan Schulte am Esch ◽  
Wolfram T. Knoefel ◽  
Michael Klein ◽  
Alireza Ghodsizad ◽  
Guenter Fuerst ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Resection ◽  
Liver Regeneration ◽  
Bone Marrow Cells ◽  
Bone Marrow Stem Cells ◽  
Stem Cell Marker ◽  
Liver Mass ◽  
Novel Concept ◽  
Marrow Cells

Abstract The liver has a large capacity for regeneration after surgical resection. However, below a critical level of future liver remnant volume, liver resection is accompanied by significant increase of postoperative liver failure and mortality. There is accumulating evidence for the contribution of hematopoetic stem cells to participate in liver regeneration. Here we report on three patients that were subjected to intra portal application of CD133+ bone marrow stem cells to support a standard concept of preoperative proliferation of the prospective liver mass. In all three cases, scheduled for right hepatic trisegmentectomy, isolated portal venous embolisation (PVE) of liver segments I and IV–VIII routinely utilized to expand left lateral hepatic segments II/III (prospective remaining liver mass) was questionably sufficient. Autologous bone marrow cells were enriched for stem cell marker CD133 utilizing an immunomagnetic device. For characterisation, each preparation of Cells was subjected to FACS-analyses for markers CD34, CD45 and CD133. Absolute numbers of 2.4 to 12.3 mio CD133+ cells were porto-venously applied to the left-lateral liver segments following PVE of contra lateral liver segments. The treatment with CD133+ was well tolerated without obvious side effects. CT-scan volumetry revealed 2.5 fold increased mean proliferation rates of left-lateral segments when compared to a group of three consecutive patients, treated with the same therapeutic concept - except the application of stem cells. Two to three weeks after intervention, future remnant liver volume was sufficient to perform expanded liver resection, which was applied safely to all patients. This first experience with a novel concept of portovenous application of CD133+ bone marrow cells subsequent to porto-venous embolisation encourages to carry out future controlled trials to evaluate the effectiveness of this approach to boost liver regeneration processes.

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