CO2 Laser Photoacoustic Spectrometer for Measuring Acetone in the Breath of Lung Cancer Patients

A CO2 laser has the advantages of being high in power and having many laser lines in the 9–11 µm infrared region. Thus, a CO2 laser photoacoustic spectrometer (PAS) can have a multi-component measurement capability for many gas compounds that have non-zero absorption coefficients at the laser lines, and therefore can be applied for measuring several volatile organic compounds (VOCs) in the human breath. We have developed a CO2 laser PAS system for detecting acetone in the human breath. Although acetone has small absorption coefficients at the CO2 laser lines, our PAS system was able to obtain strong photoacoustic (PA) signals at several CO2 laser lines, with the strongest one being at the 10P20 line. Since at the 10P20 line, ethylene and ammonia also have significant absorption coefficients, these two gases have to be included in a multi-component measurement with acetone. We obtained the lowest detection limit of our system for the ethylene, acetone, and ammonia are 6 ppbv, 11 ppbv, and 31 ppbv, respectively. We applied our PAS system to measure these three VOCs in the breath of three groups of subjects, i.e., patients with lung cancer disease, patients with other lung diseases, and healthy volunteers.

Download Full-text

Sequencing and structure analysis of UBC gene for breast and ‎lung cancer

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i3.1329 ◽

2019 ◽

Vol 10 (3) ◽

pp. 1640-1645

Author(s):

Saleen Salam Abdulhadi ◽

Abbas Abdullah Mohammed‎

Keyword(s):

Lung Cancer ◽

Cancer Patients ◽

Genetic Alterations ◽

Cancer Disease ◽

Blood Samples ◽

Lung Cancer Patients ◽

Control Subjects ◽

Exon 1 ◽

Healthy Control ◽

Transition Mutation

In the present study, sequencing approach has been adopted for exploring the ‎genetic alteration of sequences for the ubiquitin gene (UBC) in patients of breast and ‎lung cancer and comparing the results with a normal sequence that obtained from NCBI. ‎The aim of this study was to detect for genetic alterations of UBC gene in the breast and ‎lung cancer patients then compare with healthy control subjects, to investigate the ‎association between the mutations at the intron region of the UBC gene and cancer disease, ‎‎40 blood samples were examined from patients with breast and lung cancer aged ranged from (17-65) years, were collected at Al-Amal Hospital of cancer in Baghdad ‎province/Iraq, the period of collecting samples were from October/2018 to January/2019. ‎While twenty-two blood samples from healthy control subjects were collected at ages ‎ranged from(19-59). After DNA extraction, the PCR primer was designed to amplify the ‎region in the UBC gene (part of exon 1 and the whole intron). Here we report the polymorphism of the intron sequence of the UBC gene in Iraqi population as the results of sequencing the PCR amplified products showed three different transition mutation G→A, ‎C→T, T→C in patients with breast cancer were also appeared in healthy control subjects. While nine transition mutations appeared in lung cancer patients, at different locations ‎of the sequence were detected by BLAST tool. ‎

Download Full-text

Analysis of differential metabolites in lung cancer patients based on metabolomics and bioinformatics

Future Oncology ◽

10.2217/fon-2019-0818 ◽

2020 ◽

Vol 16 (18) ◽

pp. 1269-1287 ◽

Cited By ~ 2

Author(s):

Chenchen Zhao ◽

Xianbin Kong ◽

Shuang Han ◽

Xiaojiang Li ◽

Tong Wu ◽

...

Keyword(s):

Lung Cancer ◽

Cancer Patients ◽

Biological Significance ◽

Underlying Disease ◽

Multivariate Statistical ◽

Clinical Trial Registration ◽

Cancer Disease ◽

Metabolic Markers ◽

Urine Metabolites ◽

Lung Cancer Patients

Aim: Based on metabonomics, the metabolic markers of lung cancer patients were analyzed, combined with bioinformatics to explore the underlying disease mechanism. Materials & methods: Based on case–control design, using UPLC-Q-TOF/MS, urine metabolites were detected in discovery and validation set. Multivariate statistical analysis were performed to identify potential markers for lung cancer. A network analysis was constructed to integrate lung cancer disease targets with the above metabolic markers, and its possible mechanism and biological significance were explained. Results: A total of 35 potential markers were identified, 11 of which overlapped. Five key markers have a good linear correlation with serum biochemical indicators. Conclusion: The occurrence and development of lung cancer are closely related to disturbance of D-Glutamine and D-glutamate metabolism, amino acid imbalance. This test was registered on China clinical trial registration center ( www.chictr.org.cn/index.aspx ), registration number was ChiCTR1900025543.

Download Full-text

Lifestyle and Sociodemographic and Economic Characteristics of Patients with Lung Cancer in Morocco

Canadian Respiratory Journal ◽

10.1155/2020/8031541 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Imane Harkati ◽

Mohamed Kamal Hilali ◽

Nezha Oumghar ◽

Mouna Khouchani ◽

Mohamed Loukid

Keyword(s):

Lung Cancer ◽

Cancer Patients ◽

Negative Impact ◽

Demographic Factors ◽

Normal Weight ◽

World Health ◽

Cancer Disease ◽

Lung Cancer Patients ◽

Health Organization ◽

Socioeconomic And Demographic Factors

Background. Lifestyle maintenance is a crucial condition before and after lung cancer disease. According to the previous research in the scientific databases, the effect of the interaction between socioeconomic and demographic factors on the lifestyle of lung cancer patients in Southern Morocco regions remains unexamined. Accordingly, this study was designed to examine the relationship between socioeconomic factors, demographic factors, and the lifestyle of lung cancer patients. Methods. A total of 133 patients with lung cancer were divided into 103 men and 30 women with a sex ratio of 3.43 and ages varying between 28 and 82 years, and they served as informants for the study and filled in a questionnaire to provide information on their sociodemographic background, various economic characteristics, and their lifestyle. These patients have also been submitted to an anthropometric examination following the standardized procedure recommended by the World Health Organization. The survey was conducted from July 2013 to March 2015 at the Oncology and Radiotherapy Department, at Mohammed VI Hospital Center in Marrakech, Morocco. Results. The preliminary results showed that the average age of patients was 59 ± 9 years. A proportion of 81% lived in the Marrakech-Safi region and 19% lived in four other southern regions. Among the patients, 6% were smokers, while 14% were nonsmokers and 80% were ex smokers. Following the discovery of the disease, 26% revealed that they had sleep disorders and 98% were reported to have a lack of appetite. Obesity, normal weight, and underweight were also taken as criteria to categorize the patients; thus, obese informants represented 23% of the total number, those having normal weight reached 67% and the patients having underweight represented 10%. Conclusion. Sociodemographic variables and various economic characteristics were shown to have a negative impact on the lifestyle of lung cancer patients.

Download Full-text