Amperometric Biosensor Based on Zirconium Oxide/Polyethylene Glycol/Tyrosinase Composite Film for the Detection of Phenolic Compounds

An amperometric biosensor based on the enzyme polyphenoloxidase (PPO), which makes the bioelectrocatalysis of phenolic compounds, was developed and optimized using cathecol as substrate. Polyethersulphone membranes were used for enzyme immobilization. Polyphenoloxidase oxidizes monophenols (cresolase activity) and diphenols (catecholase activity) into the corresponding o-quinones; the o-quinones formed in the enzymatic catalysis are then reduced back to cathecol at 200 mV (vs. Ag, AgCl) at a platinum electrode. The polyphenoloxidase immobilized was from commercial origin or extracted from mushrooms. p-Cresol and phenol substrates were also tested. Reproducibility, response time, linearity, sensitivity, and stability of the biosensor were studied.

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Polycrystalline bismuth oxide films for development of amperometric biosensor for phenolic compounds

Biosensors and Bioelectronics ◽

10.1016/j.bios.2009.05.038 ◽

2009 ◽

Vol 24 (12) ◽

pp. 3671-3676 ◽

Cited By ~ 39

Author(s):

Dan Shan ◽

Jing Zhang ◽

Huai-Guo Xue ◽

Yong-Cai Zhang ◽

Serge Cosnier ◽

...

Keyword(s):

Phenolic Compounds ◽

Bismuth Oxide ◽

Oxide Films ◽

Amperometric Biosensor

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Effects of wood ash, calcium hydroxide and polyethylene glycol treatments on the nutrient availability and on the biological activity of tannins using gas production technique

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200021232 ◽

2007 ◽

Vol 2007 ◽

pp. 220-220

Author(s):

E.F. Nozella ◽

I.C.S. Bueno ◽

P.B. Godoy ◽

D.M.S.S. Vitti

Keyword(s):

Biological Activity ◽

Polyethylene Glycol ◽

Chemical Composition ◽

Phenolic Compounds ◽

Calcium Hydroxide ◽

Nutrient Availability ◽

Nutritive Value ◽

Gas Production ◽

Production Technique ◽

High Alkalinity

Phenolic compounds, mainly tannins, depress the nutritive value of many feedstuffs. Tannins are hydrosoluble polymers which form complexes, essentially with proteins. These complexes are broken under conditions of high acidity (pH <3.5) or high alkalinity (pH =7.5). The aim of this work was to investigate the effects of different treatments on chemical composition and tannin bioassay of tanniniferous plants from the Northeast of Brazil.

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A Highly Sensitive Hydrogen Peroxide Amperometric Biosensor Based on the Single-Layer Graphene-Enzyme Composite Film

ECS Meeting Abstracts ◽

10.1149/ma2009-01/32/1196 ◽

2009 ◽

Keyword(s):

Hydrogen Peroxide ◽

Composite Film ◽

Single Layer ◽

Amperometric Biosensor ◽

Single Layer Graphene ◽

Layer Graphene ◽

Highly Sensitive

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Effect of tannins in the rumen microbial growth measured by 32P incorporation

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200010498 ◽

2005 ◽

Vol 2005 ◽

pp. 138-138

Author(s):

P. B. Godoy ◽

I. C. S. Bueno ◽

S. L. S. Cabral Filho ◽

E. F. Nozella ◽

M. R. S. R Peçanha ◽

...

Keyword(s):

Polyethylene Glycol ◽

Phenolic Compounds ◽

Feed Intake ◽

Microbial Growth ◽

Protein Digestibility ◽

Binding Agent ◽

Sheep And Goats ◽

Synthetic Materials ◽

32P Incorporation

The phenolic compounds are substances which reduce feed intake, protein digestibility and reduce ruminal activity in sheep and goats. To reduce the effects of tannins a lot of synthetic materials, for example PEG (polyethylene glycol) is tested as tannin-binding agent, and let protein free for the digestibility. The aim of this work was to test the effect of tannins in the rumen microbial growth using the in vitro 32P incorporation technique.

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