Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions

This study aimed to assess the relationship and possible interactions between metallothioneins (MTs) and megalin (LRP-2) in different grades of oral squamous cell carcinoma (OSCC) and premalignant lesions of the oral mucosa (oral leukoplakia and oral lichen planus). The study included archived samples of 114 patients and control subjects. Protein expression was examined by immunohistochemistry and immunofluorescence, and staining quantification was performed by ImageJ software. Protein interaction in cancer tissue was tested and visualized by proximity ligation assay. Mann-Whitney and Kruskal-Wallis tests were used to determine the significance of differences between each group, whereas Pearson correlation coefficient was performed to test correlation. Expression of both proteins differed significantly between each group showing the same pattern of gradual increasing from oral lichen planus to poorly differentiated OSCC. Moreover, MTs and megalin were found to co-express and interact in cancer tissue, and their expression positively correlated within the overall study group. Findings of prominent nuclear and chromosomal megalin expression suggest that it undergoes regulated intramembrane proteolysis upon MTs binding, indicating its ability to directly affect gene expression and cellular division in cancer tissue. The data obtained point to the onco-driving potential of MTs-megalin interaction.

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Oral lichen planus and its recent management: A review

Update Dental College Journal ◽

10.3329/updcj.v10i2.50179 ◽

2020 ◽

Vol 10 (2) ◽

pp. 29-34

Author(s):

Md Ashif Iqbal ◽

Suraiya Yesmin ◽

Fathimath Maaisha ◽

Shaama Ibrahim ◽

Puja Gotame

Keyword(s):

Lichen Planus ◽

Oral Lichen Planus ◽

Cost Effective ◽

Premalignant Lesions ◽

Treatment Modalities ◽

Cochrane Library ◽

Long Run ◽

Dermatological Disease ◽

Keywords Lichen Planus ◽

Oral Lichen

Background: Oral Lichen Planus (OLP) is one of the most common dermatological disease which is present in the oral cavity. It is a chronic autoimmune, mucocutaneous disease that affects oral mucosa as well as the skin, genital mucosa and other sites of the body.Method: In this review study, various databases such as Google Scholar, PubMed Central, Hinari and Cochrane library were searched for articles with keywords lichen planus, oral lichen planus, premalignant lesions, management of Lichen planus. Articles were searched from January 2015 to 5th November 2020.Result: From the 34 articles obtained after reviewing the abstracts, most relevant 32articles were evaluated in this study.Conclusion: The etiology, pathophysiology, clinical presentation, histopathological features, diagnosis and various management for oral lichen planus is discussed. This article also compares the existing and the most recent treatment modalities that are available throughout the world that are discussed in the literatures. However, more intensive studies must be carried out to find the best treatments which are cost-effective in the long run. Update Dent. Coll. j: 2020; 10 (2): 29-34

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Expression of HER2/neu in premalignant lesions of oral cavity

10.1101/2020.07.24.20161489 ◽

2020 ◽

Author(s):

Vansh Verma ◽

Dr. Ashesh Kumar Jha ◽

Dr. Karsing Patiri ◽

Dr. Nikhil Arora

Keyword(s):

Oral Cancer ◽

Oral Cavity ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Normal Mucosa ◽

Premalignant Lesions ◽

Severe Dysplasia ◽

Positive Contribution ◽

Cytoplasmic Staining ◽

Oral Lichen

Context: Molecular alterations in premalignant lesions of oral cavity are not well known, many reports and have found increased HER2 expression to be correlated with poor prognosis in oral cancer. However,literature on expression of HER2 in premalignant lesions is limited and data is conflicting in nature. Overexpression of HER2 in premalignant lesions may denote its positive contribution in malignant transformation of these lesions. Aims: To evaluate the expression of HER2 in premalignant lesions of oral cavity. Settings and Design: In this prospective observational study of 2 months, patients attending OPD at Department of ENT and meeting the inclusion criteria were included. Methods and Material: 23 samples of Leukoplakia and 1 sample of oral lichen planus were stained by routine H&E to confirm clinical diagnosis and assess dysplasia if any, 5 samples of normal mucosa were used as control. Immunohistochemical staining for HER2 was done. ASCO/CAP 2018 guidelines were used for reporting the results. Statistical analysis used: Percentage of lesions expressing cytoplasmic or membranous expression was calculated. Results: 1 sample of Leukoplakia with severe dysplasia expressed focal membranous staining. 20% leukoplakia lesions expressed cytoplasmic staining. Staining was not observed in oral lichen planus and leucoplakia without dysplasia. Conclusions: Membranous expression in Severe dysplasia and higher expression in oral cancer is in concordance with the multistep theory of carcinogenesis. Larger studies are needed if HER2 is to be proposed as a marker for oral premalignant lesions. Significance of cytoplasmic staining in oral premalignant lesions needs to be elucidated.

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Photodynamic therapy of oral leukoplakia and oral lichen planus using methylene blue: A pilot study

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545815400052 ◽

2015 ◽

Vol 08 (01) ◽

pp. 1540005 ◽

Cited By ~ 6

Author(s):

Savarimuthu Wilfred Prasanna ◽

Ekta Ingle ◽

Prakasa Rao Aruna ◽

Chidambaranathan Pravada ◽

Dornadula Koteeswaran ◽

...

Keyword(s):

Methylene Blue ◽

Photodynamic Therapy ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Light Exposure ◽

Oral Leukoplakia ◽

Premalignant Lesions ◽

Therapeutic Modality ◽

Oral Lichen

Premalignant lesions like oral lichen planus (OLP), oral leukoplakia (OL) has a fair probability of transforming into malignancy and they are perverse toward conventional therapies. Photodynamic therapy (PDT) has been considered as an alternative/complimentary therapeutic modality for the management of premalignant lesions. In this study, methylene blue-mediated photodynamic therapy (MB-PDT) was used as a possible alternative method for the treatment of OLP and OL. A total of 15 OLP lesions and 13 OL lesions were enrolled in the study. The patients were irradiated using metal halide lamp filtered at 630 ± 10 nm, with a light exposure dose of 120 J/cm2 per sitting. For the OLP lesions, MB-PDT was performed once a week for four weeks and for the OL lesions, MB-PDT was performed twice a week for three weeks. Lesions were evaluated pre- and post- and at follow-up sessions by changes in sign and symptom scores, and size of lesions. We have observed a 53.3% of complete reduction in the treated OLP lesions and their decrease in size, sign and symptom score after treatment and at follow-up session was statistically significant. We have also observed complete response for one OL lesion of the 13 treated lesions. The result indicates that MB-PDT is an effective modality in management of OLP and OL. Among the two types of premalignancies treated with MB-PDT, OLP lesions responded much better than that of OL.

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