scholarly journals Strategies for the Immobilization of Eversa® Transform 2.0 Lipase and Application for Phospholipid Synthesis

Catalysts ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1236
Author(s):  
Bruno R. Facin ◽  
Ernestina G. Quinto ◽  
Alexsandra Valerio ◽  
Débora de Oliveira ◽  
Jose V. Oliveira ◽  
...  
Keyword(s):  
Biodiesel Production ◽  
Thermomyces Lanuginosus ◽  
Enzyme Loading ◽  
Interfacial Activation ◽  
Thermomyces Lanuginosus Lipase ◽  
Long Time ◽  
Esterification Reactions ◽  
Reaction Cycles ◽  
Opening Up ◽  
Very High

Eversa® Transform 2.0 lipase (ET2) is a recent lipase formulation derived from the Thermomyces lanuginosus lipase cultivated on Aspergillus oryzae and specially designed for biodiesel production. Since it has not been available for a long time, research on the efficiency of this enzyme in other applications remains unexplored. Moreover, even though it has been launched as a free enzyme, its immobilization may extend the scope of ET2 applications. This work explored ET2 immobilization on octadecyl methacrylate beads (IB-ADS-3) and proved the efficiency of the derivatives for esterification of glycerophosphocholine (GPC) with oleic acid in anhydrous systems. ET2 immobilized via interfacial activation on commercial hydrophobic support Immobead IB-ADS-3 showed maximum enzyme loading of 160 mg/g (enzyme/support) and great stability for GPC esterification under 30% butanone and solvent-free systems. For reusability, yields above 63% were achieved after six reaction cycles for GPC esterification. Considering the very high enzyme loading and the number of reuses achieved, these results suggest a potential application of this immobilized biocatalyst for esterification reactions in anhydrous media. This study is expected to encourage the exploration of other approaches for this enzyme, thereby opening up several new possibilities.

scholarly journals Study of Soybean Oil Hydrolysis Catalyzed by Thermomyces lanuginosus Lipase and Its Application to Biodiesel Production via Hydroesterification

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Elisa d'Avila Cavalcanti-Oliveira ◽  
Priscila Rufino da Silva ◽  
Alessandra Peçanha Ramos ◽  
Donato Alexandre Gomes Aranda ◽  
Denise Maria Guimarães Freire
Keyword(s):  
Soybean Oil ◽  
Methyl Esters ◽  
Reaction Medium ◽  
Acid Catalyst ◽  
Biodiesel Production ◽  
Thermomyces Lanuginosus ◽  
Molar Ratio ◽  
Esterification Reaction ◽  
Thermomyces Lanuginosus Lipase ◽  
Niobic Acid

The process of biodiesel production by the hydroesterification route that is proposed here involves a first step consisting of triacylglyceride hydrolysis catalyzed by lipase from Thermomyces lanuginosus (TL 100L) to generate free fatty acids (FFAs). This step is followed by esterification of the FFAs with alcohol, catalyzed by niobic acid in pellets or without a catalyst. The best result for the enzyme-catalyzed hydrolysis was obtained under reaction conditions of 50% (v/v) soybean oil and 2.3% (v/v) lipase (25 U/mL of reaction medium) in distilled water and at 60∘C; an 89% conversion rate to FFAs was obtained after 48 hours of reaction. For the esterification reaction, the best result was with an FFA/methanol molar ratio of 1:3, niobic acid catalyst at a concentration of 20% (w/w FFA), and 200∘C, which yielded 92% conversion of FFAs to soy methyl esters after 1 hour of reaction. This study is exceptional because both the hydrolysis and the esterification use a simple reaction medium with high substrate concentrations.

scholarly journals Metallo-stannosilicates as inorganic supports to immobilization of lipase from Thermomyces lanuginosus for biodiesel production

2021 ◽  
Vol 46 (1SI) ◽  
pp. 75-89
Author(s):  
Danilo Antonio Da Silva ◽  
Adriano De Vasconcellos ◽  
Jos� Geraldo Nery
Keyword(s):  
Elaeis Guineensis ◽  
Magic Angle Spinning ◽  
Ethyl Esters ◽  
Biodiesel Production ◽  
Thermomyces Lanuginosus ◽  
Free Form ◽  
Magic Angle ◽  
Nickel Ion ◽  
X Ray ◽  
Thermomyces Lanuginosus Lipase

This study reports the application of metallo-stannosilicates as potential inorganic solid matrixes for enzymes immobilization and their use as a heterogenous catalysts in enzymatic transesterification reactions for the conversion of triacylglycerides into fatty acid ethyl esters (FAEEs). Several stannosilicates were synthesized and physicochemical characterized by X-ray powder diffraction (XRD), scanning electron microscopy with energy dispersive X-ray spectrometry (SEM-EDS), Brunauer-Emmett-Teller (BET)-N2 surface area analysis and solid-state magic-angle spinning nuclear magnetic resonance (MAS NMR 29Si and 119Sn nuclei) techniques. The experimental results for enzymes immobilization were promising, especially for a nickel ion-exchanged metallo-stannosilicate, which were able to immobilize 82 +- 6% of Thermomyces lanuginosus lipase and also kept a high enzymatic activity (42 +- 3 U mg�1). Systematic catalytic reactions for conversion of refined palm oil (Elaeis guineensis) using some of these stannosilicates enzymes complexes yielded 63.3 +- 0.7% of FAEEs. It is worth noticing that, when the transesterification reaction was performed with (a) the as-made stannosilicate without enzymes and (b) the equivalent amount of immobilized Thermomyces lanuginosus lipase in its free form, the FAEEs yield sharply decreased to less than 5.0% and 6.3 +- 0.3%, respectively. This result is a clear evidence of a synergistic effect among the metallo-stannosilicates and the immobilized enzymes.

Promoting protein self-association in non-glycosylated Thermomyces lanuginosus lipase based on crystal lattice contacts

2015 ◽  
Vol 1854 (12) ◽  
pp. 1914-1921 ◽  
Author(s):  
Jens Kvist Madsen ◽  
Thomas Rebsdorf Sørensen ◽  
Jørn Døvling Kaspersen ◽  
Maria Berggård Silow ◽  
Jesper Vind ◽  
...  
Keyword(s):  
Crystal Lattice ◽  
Thermomyces Lanuginosus ◽  
Thermomyces Lanuginosus Lipase ◽  
Self Association

scholarly journals Production of Structured Phosphatidylcholine with High Content of Myristic Acid by Lipase-Catalyzed Acidolysis and Interesterification

Catalysts ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 281 ◽  
Author(s):  
Anna Chojnacka ◽  
Witold Gładkowski
Keyword(s):  
Myristic Acid ◽  
Rhizomucor Miehei ◽  
Egg Yolk ◽  
Thermomyces Lanuginosus ◽  
Molar Ratio ◽  
Acyl Donor ◽  
Thermomyces Lanuginosus Lipase ◽  
Screening Experiments ◽  
Miehei Lipase ◽  
Acyl Donors

Synthesis of structured phosphatidylcholine (PC) enriched with myristic acid (MA) was conducted by acidolysis and interesterification reactions using immobilized lipases as catalysts and two acyl donors: trimyristin (TMA) isolated from ground nutmeg, and myristic acid obtained by saponification of TMA. Screening experiments indicated that the most effective biocatalyst for interesterification was Rhizomucor miehei lipase (RML), whereas for acidolysis, the most active were Thermomyces lanuginosus lipase (TLL) and RML. The effect of the molar ratio of substrates (egg-yolk PC/acyl donor), enzyme loading, and different solvent on the incorporation of MA into PC and on PC recovery was studied. The maximal incorporation of MA (44 wt%) was achieved after 48 h of RML-catalyzed interesterification in hexane using substrates molar ratio (PC/trimyristin) 1/5 and 30% enzyme load. Comparable results were obtained in toluene with 1/3 substrates molar ratio. Interesterification of PC with trimyristin resulted in significantly higher MA incorporation than acidolysis with myristic acid, particularly in the reactions catalyzed by RML.

scholarly journals Albian drilling's and its hydropower potential in Algeria: Study and exploitation

2018 ◽  
Vol 38 (1) ◽  
pp. 8-15
Author(s):  
Salim Etsouri ◽  
Ferhat Kaci ◽  
Mohamed Bouaziz
Keyword(s):  
Sustainable Development ◽  
Flow Rate ◽  
Underground Water ◽  
High Flow ◽  
Aquifer System ◽  
Output Pressure ◽  
Turbo Generator ◽  
Hydropower Potential ◽  
Long Time ◽  
Very High

The Continental intercalary groundwater is highly sought for its water as resources hugely mobilized in Northern Sahara. A very high flow rate and output pressure characterizes this underground water. It amounts from 50 to 400 l.s-1 for the flow, and from 5 to 40 bar for pressure. A survey of the Northern Sahara Aquifer System was essential to prove the existence of this potential. This energy appears into the artesian form, which remains very considerable for a very long time in most drilling. We have realised that this energy is immense, as well as the expanded volume of the groundwater, and the importance of its use in agriculture. Unfortunately, this potential remains untapped to this day and the energy of this water is completely neglected. Several turbo generator and/or inverted pump (PATs) integration tests were undergone. The new concept of reflection with respect to the environment and sustainable development has led us to structure our work towards the extension of this potential in order to extract the exploitable energy.

scholarly journals Upaya Konservasi Tanah dan Air pada Daerah Pertanian Dataran Tinggi di Sub-Daerah Aliran Sungai Gandul

2020 ◽  
Vol 25 (3) ◽  
pp. 382-387
Author(s):  
Diah Auliyani
Keyword(s):  
Water Conservation ◽  
Soil And Water Conservation ◽  
Conservation Practices ◽  
Land System ◽  
Long Time ◽  
Agricultural Areas ◽  
Land Cover Maps ◽  
Very High ◽  
Soil And Water ◽  
Erosion Prevention

Land susceptibility to degradation is characterized by the loss of topsoil due to erosion which is considered as a threat to agricultural productivity. Information about land sensitivity to erosion is crucial in determining the appropriate soil and water conservation techniques to avoid land degradation. This study, which was located in the Gandul Sub-Watershed, aims to analyze the level of land sensitivity to erosion in highland agricultural areas. Data analysis was carried out spatially using land system and land-cover maps. Soil and water conservation efforts were determined based on the land function and sensitivity. The land sensitivity to erosion in the Gandul Sub-watershed was categorized into 3 levels which were moderate (3.9%), high (95%), and very high (0.8%). There were various directions for Gandul Sub-Watershed management by considering the level of sensitivity of the land and the function of the area. Creating a ridge is one of the soils and water conservation practices that has been implemented for a long time by local communities as a soil erosion prevention.   Keywords: agriculture, erosion, Gandul, sensitivity

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