scholarly journals Responses of Polyamine-Metabolic Genes to Polyamines and Plant Stress Hormones in Arabidopsis Seedlings

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3283
Author(s):  
Yusaku Yariuchi ◽  
Takashi Okamoto ◽  
Yoshiteru Noutoshi ◽  
Taku Takahashi
Keyword(s):  
Methyl Jasmonate ◽  
Plant Hormones ◽  
Amine Oxidase ◽  
Stress Hormones ◽  
Plant Stress ◽  
Polyamine Metabolism ◽  
Negative Feedback Regulation ◽  
Oxidase Gene ◽  
Spermine Synthase ◽  
Metabolic Genes

In plants, many of the enzymes in polyamine metabolism are encoded by multiple genes, whose expressions are differentially regulated under different physiological conditions. For comprehensive understanding of their regulation during the seedling growth stage, we examined the expression of polyamine metabolic genes in response to polyamines and stress-related plant hormones in Arabidopsis thaliana. While confirming previous findings such as induction of many of the genes by abscisic acid, induction of arginase genes and a copper amine oxidase gene, CuAOα3, by methyl jasmonate, that of an arginine decarboxylase gene, ADC2, and a spermine synthase gene, SPMS, by salicylic acid, and negative feedback regulation of thermospermine biosynthetic genes by thermospermine, our results showed that expressions of most of the genes are not responsive to exogenous polyamines. We thus examined expression of OsPAO6, which encodes an apoplastic polyamine oxidase and is strongly induced by polyamines in rice, by using the promoter-GUS fusion in transgenic Arabidopsis seedlings. The GUS activity was increased by treatment with methyl jasmonate but neither by polyamines nor by other plant hormones, suggesting a difference in the response to polyamines between Arabidopsis and rice. Our results provide a framework to study regulatory modules directing expression of each polyamine metabolic gene.

Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha

1989 ◽  
Vol 1008 (2) ◽  
pp. 157-167 ◽  
Author(s):  
Paul G Bruinenberg ◽  
Melchior Evers ◽  
Hans R Waterham ◽  
Jeroen Kuipers ◽  
Annika C Arnberg ◽  
...  
Keyword(s):  
Amine Oxidase ◽  
Hansenula Polymorpha ◽  
Cloning And Sequencing ◽  
Oxidase Gene

scholarly journals Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)

2021 ◽  
pp. 39-43
Author(s):  
Ákos Mendel ◽  
László Kovács ◽  
Erzsébet Kiss
Keyword(s):  
Nicotiana Tabacum ◽  
Salt Tolerance ◽  
Lower Class ◽  
Arginine Decarboxylase ◽  
Polyamine Metabolism ◽  
Spermine Synthase ◽  
Nicotiana Tabacum L ◽  
Exogenous Addition ◽  
Precursor Molecules

Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital and essential aliphatic amines which are also present in plants. Although ethylene and polyamines are also involved in fruit ripening, the genes coding them must also take part in other biosynthetic pathways. In the ethylene and polyamines play an important role in development of salt stress tolerance, and in responses for biotic and abiotic stresses. Exogenous application of all three main polyamines (Put, Spd, Spm) increase salt tolerance of plants, but, accordingly to previous experiments, spermidine has the main effect on the enhancement of salt tolerance. Nicotiana tabacum L. plants were grown in vitro on MS medium, the treatments were as follows: arginine (150 mg l-1), putrescine (10 mg l-1), spermidine (10 mg l-1). Proline, chlorophyll a, b and polyamine contents were measured. The obtained results show that the arginine decarboxylase and the spermidine synthase genes involved in polyamine metabolism, cannot be enhanced by exogenous addition of their precursor molecules. On the contrary, the spermine synthase gene has a positive effect to the lower-class forms of polyamines.

scholarly journals An amine oxidase gene from mud crab, Scylla paramamosain, regulates the neurotransmitters serotonin and dopamine in vitro

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0204325
Author(s):  
Junguo Liu ◽  
Ming Zhao ◽  
Wei Song ◽  
Lingbo Ma ◽  
Xiu Li ◽  
...  
Keyword(s):  
Amine Oxidase ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Oxidase Gene

Polyamine metabolism during cardiac hypertrophy

1980 ◽  
Vol 239 (5) ◽  
pp. E372-E378 ◽  
Author(s):  
A. E. Pegg ◽  
H. Hibasami
Keyword(s):  
Cardiac Hypertrophy ◽  
Polyamine Metabolism ◽  
Decarboxylase Activity ◽  
Elevated Concentration ◽  
Enzyme Protein ◽  
Carboxylase Activity ◽  
Rapid Reduction ◽  
Spermine Synthase

Treatment with thyroxine for 7 days to produce myocardial hypertrophy led to an increase in the content of putrescine, spermidine, and spermine in the rat heart. The content of decarboxylated S-adenosylmethionine, the source of the aminopropyl groups needed for polyamine synthesis, was increased by the thyroxine treatment as were the activities of ornithine and S-adenosylmethionine decarboxylases. The enhanced S-adenosylmethionine decarboxylase activity measured in vitro was due to an increase in the amount of enzyme protein as measured by immunotitration with a specific antiserum. In vivo, decarboxylation of S-adenosylmethionine was, therefore, increased both by the increased amount of enzyme protein and by the elevated concentration of putrescine (which activates the enzyme) brought about by the enhanced ornithine carboxylase activity. Spermine synthase did not change significantly during the treatment and spermidine synthase increased only slightly. Therefore, the accumulation of polyamines was mediated predominantly via the increased availability of both putrescine and decarboxylated S-adenosylmethionine. Administration of 1,3-diamino-2-propanol led to a rapid reduction in the activity of ornithine decarboxylase in the heart, and continued exposure to this substance by its inclusion in the drinking water completely prevented the increase in concentration of putrescine and polyamines in response to thyroxine. However, cardiac hypertrophy as measured by the increase in cardiac mass was not prevented by such treatment with 1,3-diaminopropanol, showing that the increased content of polyamines was not essential for the hypertrophic response.

scholarly journals Bioactive role of plant stress hormone methyl jasmonate against lipopolysaccharide induced arthritis

Heliyon ◽  
2020 ◽  
Vol 6 (11) ◽  
pp. e05432
Author(s):  
S.M. Gunjegaonkar ◽  
S.B. Wankhede ◽  
T.S. Shanmugarajan ◽  
S.D. Shinde
Keyword(s):  
Methyl Jasmonate ◽  
Plant Stress ◽  
Stress Hormone

scholarly journals Chemical sensing of plant stress at the ecosystem scale

2008 ◽  
Vol 5 (5) ◽  
pp. 1287-1294 ◽  
Author(s):  
T. Karl ◽  
A. Guenther ◽  
A. Turnipseed ◽  
E. G. Patton ◽  
K. Jardine
Keyword(s):  
Plant Hormones ◽  
Systemic Acquired Resistance ◽  
Chilling Injury ◽  
Plant Stress ◽  
Acquired Resistance ◽  
Warning Signal ◽  
Ambient Air ◽  
Aerosol Formation ◽  
Night Time ◽  
The Impact

Abstract. Significant ecosystem-scale emissions of methylsalicylate (MeSA), a semivolatile plant hormone thought to act as the mobile signal for systemic acquired resistance (SAR), were observed in an agroforest. Our measurements show that plant internal defence mechanisms can be activated in response to temperature stress and are modulated by water availability on large scales. Highest MeSA fluxes (up to 0.25 mg/m2/h) were observed after plants experienced ambient night-time temperatures of ~7.5°C followed by a large daytime temperature increase (e.g. up to 22°C). Under these conditions estimated night-time leaf temperatures were as low as ~4.6°C, likely inducing a response to prevent chilling injury. Our observations imply that plant hormones can be a significant component of ecosystem scale volatile organic compound (VOC) fluxes (e.g. as high as the total monoterpene (MT) flux) and therefore contribute to the missing VOC budget. If generalized to other ecosystems and different types of stresses these findings suggest that semivolatile plant hormones have been overlooked by investigations of the impact of biogenic VOCs on aerosol formation events in forested regions. Our observations show that the presence of MeSA in canopy air serves as an early chemical warning signal indicating ecosystem-scale stresses before visible damage becomes apparent. As a chemical metric, ecosystem emission measurements of MeSA in ambient air could therefore support field studies investigating factors that adversely affect plant growth.

scholarly journals Cloning of a Novel Nicotine Oxidase Gene from Pseudomonas sp. Strain HZN6 Whose Product Nonenantioselectively Degrades Nicotine to Pseudooxynicotine

2013 ◽  
Vol 79 (7) ◽  
pp. 2164-2171 ◽  
Author(s):  
Jiguo Qiu ◽  
Yun Ma ◽  
Jing Zhang ◽  
Yuezhong Wen ◽  
Weiping Liu
Keyword(s):  
Amine Oxidase ◽  
Sole Source ◽  
Site Directed Mutagenesis ◽  
Broad Host Range ◽  
Upstream Sequence ◽  
Oxidase Gene ◽  
Content Type ◽  
Reading Frame ◽  
Degrading Bacteria ◽  
Nicotine Degradation

ABSTRACTPseudomonassp. strain HZN6 utilizes nicotine as its sole source of carbon, nitrogen, and energy. However, its catabolic mechanism has not been elucidated. In this study, self-formed adaptor PCR was performed to amplify the upstream sequence of the pseudooxynicotine amine oxidase gene. A 1,437-bp open reading frame (designatednox) was found to encode a nicotine oxidase (NOX) that shows 30% amino acid sequence identity with 6-hydroxy-l-nicotine oxidase fromArthrobacter nicotinovorans. Thenoxgene was cloned into a broad-host-range cloning vector and transferred into the non-nicotine-degrading bacteriaEscherichia coliDH5α (DH-nox) andPseudomonas putidaKT2440 (KT-nox). The transconjugant KT-nox obtained nicotine degradation ability and yielded an equimolar amount of pseudooxynicotine, while DH-nox did not. Reverse transcription-PCR showed that thenoxgene is expressed in both DH5α and KT2440, suggesting that additional factors required for nicotine degradation are present in aPseudomonasstrain(s), but not inE. coli. The mutant of strain HZN6 withnoxdisrupted lost the ability to degrade nicotine, but not pseudooxynicotine. These results suggested that thenoxgene is responsible for the first step of nicotine degradation. The (RS)-nicotine degradation results showed that the two enantiomers were degraded at approximately the same rate, indicating that NOX does not show chiral selectivity. Site-directed mutagenesis revealed that both the conserved flavin adenine dinucleotide (FAD)-binding GXGXXG motif and His456 are essential for nicotine degradation activity.

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