A Coumarin-Benzothiazole Derivative as a FRET-Based Chemosensor of Adenosine 5′-Triphosphate

A coumarin-benzothiazole ratiometric probe of ATP was designed and synthesized. The probe is based on incorporation of benzothiazole scaffold as a donor and coumarin nucleus as an acceptor in a single Förster resonance energy transfer/fluorescence resonance energy transfer (FRET) sensing platform. The sensor can detect ATP in aqueous solution with high selectivity over other nucleotide polyphosphate (NPP) anions. Binding of ATP to the sensor results in modulation of FRET efficiency between the donor and the acceptor which afforded a linear relationship between FRET signal and ATP (0.1–10 μM). A limit of detection (LOD) of 94.5 nM was quantified for FRET sensing of ATP by the probe. In addition, Job plot analysis revealed 1:1 binding interaction between the probe and ATP. The FRET probe was successfully utilized in monitoring ATP hydrolysis by apyrase in aqueous solution.

Download Full-text

Peptide-coated palladium nanoparticle for highly sensitive bioanalysis of trypsin in human urine samples

Nanomaterials and Nanotechnology ◽

10.1177/1847980418820391 ◽

2018 ◽

Vol 8 ◽

pp. 184798041882039 ◽

Cited By ~ 7

Author(s):

Guohua Zhou ◽

Huimin Jiang ◽

Yanfang Zhou ◽

Peilian Liu ◽

Yongmei Jia ◽

...

Keyword(s):

Energy Transfer ◽

Fluorescence Resonance Energy Transfer ◽

Palladium Nanoparticles ◽

Limit Of Detection ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Water Soluble ◽

Urine Samples ◽

Fluorescence Resonance ◽

Functional Peptide

In recent years, palladium nanoparticles have been proved as energy acceptor candidates in fluorescence resonance energy transfer-based sensors for analytical and biological purposes. In this article, peptide-coated palladium nanoparticles were prepared using a simple one-step preparation method. The peptide Cys-Ala-Leu-Asn-Asn was used as a ligand, whereas hydrazine hydrate was used as a reductant to obtain water-soluble and stable peptide-coated palladium nanoparticles. Additionally, peptide-coated palladium nanoparticles were functionalized by adding the functional peptide CALNNGGARK(FITC) in combination with Cys-Ala-Leu-Asn-Asn during the preparation process. The prepared functionalized peptide-coated palladium nanoparticles were used for trypsin detection based on the fluorescence resonance energy transfer approach. Under optimized conditions, the proposed method can be used for the detection of trypsin concentrations in the range of approximately 0.2–8-μg/mL with a limit of detection of 0.18-μg/mL. The functionalized peptide-coated palladium nanoparticles were successfully applied for the detection of trypsin in urine samples. Our findings also indicated that peptide-coated palladium nanoparticles can highly quench fluorophores and are suitable for the manufacture of off–on state fluorescent sensors. We anticipated that the peptide-coated palladium nanoparticles proposed in this article will have great potential for the detection of trypsin in urine and other analytical, biological, and clinical applications.

Download Full-text