Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits

Soft gingival tissue deficiency remains a severe problem leading to postoperative recession, peri-implantitis, and bone resorption. The use of collagen matrices does not always lead to complete rebuilding of the gingiva volume. The application of mesenchymal stromal cells (MSCs) simultaneously with collagen materials represents a promising approach for the restoration of soft gingival tissues. However, short-term effects of MSCs-enriched collagen grafts after gingival augmentation have not yet been studied properly. Mucograft and Mucoderm matrices were implanted in rabbits (n = 12) simultaneously with the intraoperative injection of rabbit bone marrow-derived mesenchymal stromal cells (BM-MSCs) or without cells. Collagen matrices were implanted under the flap or by the surface technique without intentional primary closure. The samples were harvested seven days after implantation, histological staining with hematoxylin and eosin, and immunohistochemical staining for VEGF, IGF1, and TGF were performed. The use of Mucoderm led to better augmentation outcomes on day 7 compared with Mucograft (p < 0.0001). Gingival augmentation in combination with the local administration of BM-MSCs led to better regeneration of the soft gingival tissues independently of the type of implanted collagen matrices (p < 0.0001). Furthermore, injection of BM-MSCs significantly enhanced gingival vascularization and epithelization with a clear positive correlation between vascular growth and epithelial response. Administration of BM-MSCs in combination with various collagen materials may potentially improve gingiva regeneration.

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Comparative analysis of mesenchymal stromal cells derived from rabbit bone marrow and Wharton’s jelly for adipose tissue engineering

Connective Tissue Research ◽

10.1080/03008207.2019.1631297 ◽

2019 ◽

Vol 61 (6) ◽

pp. 537-545

Author(s):

Linli Li ◽

Jian Dong ◽

Yiqun He ◽

Wei Mao ◽

Han Tang ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Adipose Tissue ◽

Comparative Analysis ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Wharton’S Jelly ◽

Adipose Tissue Engineering ◽

Wharton's Jelly ◽

Rabbit Bone

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Sulfated Hyaluronan Containing Collagen Matrices Enhance Cell-Matrix-Interaction, Endocytosis, and Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Journal of Proteome Research ◽

10.1021/pr300640h ◽

2012 ◽

Vol 12 (1) ◽

pp. 378-389 ◽

Cited By ~ 39

Author(s):

Stefanie Kliemt ◽

Claudia Lange ◽

Wolfgang Otto ◽

Vera Hintze ◽

Stephanie Möller ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Collagen Matrices ◽

Human Mesenchymal Stromal Cells ◽

Matrix Interaction ◽

Cell Matrix

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Morphological Features of Mesenhymal Stroma Cells of Chorionic Villi

Annals of the Russian academy of medical sciences ◽

10.15690/vramn767 ◽

2017 ◽

Vol 72 (1) ◽

pp. 76-83 ◽

Cited By ~ 1

Author(s):

N. V. Nizyaeva ◽

Т. V. Sukhacheva ◽

G. V. Kulikova ◽

M. N. Nagovitsyna ◽

N. E. Kan ◽

...

Keyword(s):

Electron Microscopy ◽

Cell Culture ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Placental Tissue ◽

Control Group ◽

Chorionic Villi ◽

Mean Values ◽

Hematoxylin And Eosin ◽

Hematoxylin And Eosin Staining

Background: Nowadays autologous mesenchymal placental stromal cells (MSCs) may use to treat for various diseases both of the mother and the child. Stroma of the placenta villi is appropriated origin for cell culture isolation. Aim of the study was to evaluate the possibility for selection and use of placental tissue for mesenchymal stromal cells. Materials and methods: The present study was based on 45 placental samples of women aged 27−38 yy. who underwent surgical delivery at 36−40 weeks of gestation. 30 of these women have been enrolled in the basic group including children with congenital abnormalities (CA). The comparison group consisted of 15 patients with physiological pregnancy. We performed histological examination (with hematoxylin and eosin staining), immunohistochemical examination (with use monoclonal antibodies CD90 (1:25; Abcam, UK), СD105 (1:500; Abcam, UK), CD44 (1:25; Dako), СD73 (1:200, Abcam, UK), and electron microscopy (by microscope Philips/FEI Corporation, Eindhoven, Holland). Eclipse 80i microscope (Nikon Corporation, Japan) was used to examine the immunohistochemical reactions as a brown staining. The evaluation of the intensity of reaction was conducted by NIS-Elements Advanced Research 3.2 program (Czech Republic). Student’s t-test and analysis of variance were used to compare the mean values. Differences were considered statistically significant at p0.05. Results: Interstitial cells of the stroma of the villi with CA had fibroblastic differentiation as revealed degenerative changes of the cells. The histologic examination with hematoxylin and eosin staining revealed significant fibrosis of the stroma of the placenta villi in CA group (p0,01). Immunohistochemical study of stem and intermediate chorionic villi revealed no significant differences in staining of CD44+, СD90+, СD73+, and CD105+ cells if compared to the control group (p0.05). Although CD105 expression was significantly lower in the CA group (0.058±0.0049) than in the control group (0.088±0.0039) (p0.05). However, electron microscopy detected the villi interstitial stromal cells with fibroblastic differentiation in CA group. Conclusions: Thus, it is necessary to exclude placenta with obstetrical history, somatic, and congenital pathology of the mother and the child when selecting the placental cell culture. Moreover, choosing a sample the morphological structure of the placenta should be taken into consideration. However, congenital malformations of the fetus, pathology of the mother cultivate mesenchymal stromal cells of placentas is inappropriate and should be taken advantage of the donor cells.

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