Comparative analysis of mesenchymal stromal cells derived from rabbit bone marrow and Wharton’s jelly for adipose tissue engineering

2019 ◽  
Vol 61 (6) ◽  
pp. 537-545
Author(s):  
Linli Li ◽  
Jian Dong ◽  
Yiqun He ◽  
Wei Mao ◽  
Han Tang ◽  
...  
Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2163
Author(s):  
Xing Liu ◽  
Adrien Baldit ◽  
Emilie de Brosses ◽  
Frédéric Velard ◽  
Ghislaine Cauchois ◽  
...  

(1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering.


Cytotherapy ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Sudha Balasubramanian ◽  
Parvathy Venugopal ◽  
Swathi Sundarraj ◽  
Zubaidah Zakaria ◽  
Anish Sen Majumdar ◽  
...  

FEBS Open Bio ◽  
2016 ◽  
Vol 6 (11) ◽  
pp. 1054-1066 ◽  
Author(s):  
Claire Mennan ◽  
Sharon Brown ◽  
Helen McCarthy ◽  
Eleni Mavrogonatou ◽  
Dimitris Kletsas ◽  
...  

2015 ◽  
Vol 24 (22) ◽  
pp. 2649-2659 ◽  
Author(s):  
Mark van der Garde ◽  
Melissa van Pel ◽  
Jose Eduardo Millán Rivero ◽  
Alice de Graaf-Dijkstra ◽  
Manon C. Slot ◽  
...  

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