scholarly journals Muscles in Winter: The Epigenetics of Metabolic Arrest

Epigenomes ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 28
Author(s):  
W. Aline Ingelson-Filpula ◽  
Kenneth B. Storey

The winter months are challenging for many animal species, which often enter a state of dormancy or hypometabolism to “wait out” the cold weather, food scarcity, reduced daylight, and restricted mobility that can characterize the season. To survive, many species use metabolic rate depression (MRD) to suppress nonessential metabolic processes, conserving energy and limiting tissue atrophy particularly of skeletal and cardiac muscles. Mammalian hibernation is the best recognized example of winter MRD, but some turtle species spend the winter unable to breathe air and use MRD to survive with little or no oxygen (hypoxia/anoxia), and various frogs endure the freezing of about two-thirds of their total body water as extracellular ice. These winter survival strategies are highly effective, but create physiological and metabolic challenges that require specific biochemical adaptive strategies. Gene-related processes as well as epigenetic processes can lower the risk of atrophy during prolonged inactivity and limited nutrient stores, and DNA modifications, mRNA storage, and microRNA action are enacted to maintain and preserve muscle. This review article focuses on epigenetic controls on muscle metabolism that regulate MRD to avoid muscle atrophy and support winter survival in model species of hibernating mammals, anoxia-tolerant turtles and freeze-tolerant frogs. Such research may lead to human applications including muscle-wasting disorders such as sarcopenia, or other conditions of limited mobility.

1999 ◽  
Vol 21 (2) ◽  
pp. 159-164 ◽  
Author(s):  
K.R. Tiwari ◽  
T.D. Warkentin ◽  
G.A. Penner ◽  
J.G. Menzies

2011 ◽  
Vol 57 (8) ◽  
pp. 1115-1122 ◽  
Author(s):  
Guijun Zhang ◽  
Janet M. Storey ◽  
Kenneth B. Storey

1990 ◽  
Vol 68 (2) ◽  
pp. 617-626 ◽  
Author(s):  
R. J. Rose ◽  
D. R. Hodgson ◽  
T. B. Kelso ◽  
L. J. McCutcheon ◽  
W. M. Bayly ◽  
...  

Changes in blood gases, ions, lactate, pH, hemoglobin, blood temperature, total body metabolism, and muscle metabolites were measured before and during exercise (except muscle), at fatigue, and during recovery in normal and acetazolamide-treated horses to test the hypothesis that an acetazolamide-induced acidosis would compromise the metabolism of the horse exercising at maximal O2 uptake. Acetazolamide-treated horses had a 13-mmol/l base deficit at rest, higher arterial Po2 at rest and during exercise, higher arterial and mixed venous Pco2 during exercise, and a 48-s reduction in run time. Arterial pH was lower during exercise but not in recovery after acetazolamide. Blood temperature responses were unaffected by acetazolamide administration. O2 uptake was similar during exercise and recovery after acetazolamide treatment, whereas CO2 production was lower during exercise. Muscle [glycogen] and pH were lower at rest, whereas heart rate, muscle pH and [lactate], and plasma [lactate] and [K+] were lower and plasma [Cl-] higher following exercise after acetazolamide treatment. These data demonstrate that acetazolamide treatment aggravates the CO2 retention and acidosis occurring in the horse during heavy exercise. This could negatively affect muscle metabolism and exercise capacity.


2010 ◽  
Vol 109 (6) ◽  
pp. 1989-1995 ◽  
Author(s):  
Samuel N. Cheuvront ◽  
Robert W. Kenefick ◽  
Scott J. Montain ◽  
Michael N. Sawka

Environmental heat stress can challenge the limits of human cardiovascular and temperature regulation, body fluid balance, and thus aerobic performance. This minireview proposes that the cardiovascular adjustments accompanying high skin temperatures (Tsk), alone or in combination with high core body temperatures (Tc), provide a primary explanation for impaired aerobic exercise performance in warm-hot environments. The independent (Tsk) and combined (Tsk + Tc) effects of hyperthermia reduce maximal oxygen uptake (V̇o2max), which leads to higher relative exercise intensity and an exponential decline in aerobic performance at any given exercise workload. Greater relative exercise intensity increases cardiovascular strain, which is a prominent mediator of rated perceived exertion. As a consequence, incremental or constant-rate exercise is more difficult to sustain (earlier fatigue) or requires a slowing of self-paced exercise to achieve a similar sensation of effort. It is proposed that high Tsk and Tc impair aerobic performance in tandem primarily through elevated cardiovascular strain, rather than a deterioration in central nervous system (CNS) function or skeletal muscle metabolism. Evaporative sweating is the principal means of heat loss in warm-hot environments where sweat losses frequently exceed fluid intakes. When dehydration exceeds 3% of total body water (2% of body mass) then aerobic performance is consistently impaired independent and additive to heat stress. Dehydration augments hyperthermia and plasma volume reductions, which combine to accentuate cardiovascular strain and reduce V̇o2max. Importantly, the negative performance consequences of dehydration worsen as Tsk increases.


Specific biochemical adaptations permit winter survival at subzero temperatures by both freeze-tolerant and freeze-avoiding insects. Common to both survival strategies is the accumulation of high concentrations of polyols, providing deep supercooling point depression for freeze-avoiding forms and regulating cell volume reduction during extracellular freezing in freeze-tolerant insects. Studies in my laboratory have elucidated the molecular mechanisms (temperature effects on enzyme properties, allosteric regulation, reversible protein phosphorylation) that control the massive conversion of glycogen to polyols and, in some species, regulate the differential synthesis of dual polyols. New studies have highlighted the importance of aerobic ATP production for glycerol biosynthesis, suggested the importance of microcompartmentation for optimal conversion efficiency, documented seasonal changes in the capacity for polyol synthesis versus reconversion to glycogen and analysed the role of protein phosphorylation in enzyme regulation during polyol synthesis.


Author(s):  
Solange Akhere Gwan ◽  
Victor Konfor Ntoban ◽  
Jude N. Kimengsi

Mountainous regions and other difficult terrains, the world over, present significant challenges to communities as they strive to carry out their daily activities. In spite of these difficulties, strategies have been employed by communities to cope with such difficulties, yielding diverse outcomes. The extent and outcomes of survival strategies employed by communities, still beg for scientific and policy edification, in the context of the Western Highlands of Cameroon. This paper contributes to bridge the knowledge gap, by examining the survival strategies employed by locals in the Kom Highlands to confront the challenges presented by the harsh physical environment. 10 key informant interviews were conducted accompanied by a representative survey of 60 farming household heads, drawn from 5 villages in Fundong. The data were analyzed using both descriptive and inferential statistical tools, including the Chi-square analysis. The results reveal that Kom displays a plethora of harsh physical environmental characteristics, prominent among them are the hilly and difficult terrain, the poor soil quality and the generally cold weather conditions witnessed here. Faced with these challenges, the population employed a number of survival strategies in the agricultural sector, housing and transport. These strategies are unfortunately inadequate and such inadequacy is accounted for by their low level of technology, poverty, ignorance and other cultural factors, among others. The study therefore recommends the need to improve and modernize agriculture through the provision of fertilizers at subsidized rates to the farming population, the encouragement of effective slope stabilization and terracing and also for rigorous government intervention in terms of road and fly over constructions.


Geography ◽  
2014 ◽  
Author(s):  
Lois M. Takahashi

Homelessness has been defined along a spectrum of insufficient and inadequate shelter, from literal street sleeping to sleeping in temporary shelters to overcrowded housing circumstances; along a spectrum of time, from continuous to sporadic homeless episodes; and along a spectrum of space, from limited mobility to movement within and across geographic areas. In the United States and the Western industrialized world, homelessness was often framed as a crisis in the 1980s but has since become part of a larger narrative concerning entrenched poverty and income inequality. Research in the 1980s, primarily in sociology, psychology, social work, public policy, urban planning, public health, and geography, focused on defining homelessness, identifying the multiple and intersecting causes of homelessness, clarifying mental health issues faced by homeless persons, and recommending strategies, including emergency shelters, transitional housing, permanent housing with and without social services, and housing with and without prerequisites. Early-21st-century research on homelessness has deepened scholarly and policy understanding of the variety of homeless subpopulations and their specific needs and survival strategies and increasingly has framed homelessness as a particular aspect of the larger structural issues defining poverty and inequality in industrialized countries. Geographers in particular have emphasized the spatial dimensions of homelessness and have provided a countervailing explanation (usually based in social structures such as poverty and social exclusion) to the popular notion that homelessness is the result of individual counterproductive behavior or vulnerabilities. This review article includes both conceptual and empirical research, endeavoring to cover the myriad of conceptual frameworks explaining homelessness and the varied approaches researchers have tested to ameliorate homelessness. The article also summarizes resistant threads of scholarship on homelessness, from theories of revanchism to feminism, and includes both work in the United States and other industrialized countries (in particular, Canada and the United Kingdom). The article is organized into the following sections, which highlight in particular the contributions made by geographers and those with spatial lenses. First, two overview sections summarize publications that have led the field in conceptualizing homelessness for scholars and policymakers; one of the sections highlights specific geographical contributions. A section on reference resources, especially online, follows that and focuses on advocacy organizations. The next few sections show how scholars have described the needs and challenges faced by varying groups of homeless persons, centering on health, mental health, and substance abuse. Geographers and others have worked to reconceptualize homelessness, from descriptions of need and individual deficits to a focus on systems and politics; one section highlights these innovative works. The final four sections summarize alternatives to the population descriptions that comprise the mainstay of homelessness research to focus on stigma and identity, revanchism and resistance, and conceptual and empirical discussions of policies and programs that should be, and have been, developed and delivered to address homelessness. I thank Nathaniel Barlow for expert research assistance, and the editorial team and two anonymous reviewers for their very helpful comments. All errors or omissions remain my responsibility.


2004 ◽  
Vol 122 (3) ◽  
pp. 207-220 ◽  
Author(s):  
P.J Waller ◽  
L Rudby-Martin ◽  
B.L Ljungström ◽  
A Rydzik

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1834 ◽  
Author(s):  
Victoria E.M. Gerber ◽  
Sanoji Wijenayake ◽  
Kenneth B. Storey

The common wood frog,Rana sylvatica, utilizes freeze tolerance as a means of winter survival. Concealed beneath a layer of leaf litter and blanketed by snow, these frogs withstand subzero temperatures by allowing approximately 65–70% of total body water to freeze. Freezing is generally considered to be an ischemic event in which the blood oxygen supply is impeded and may lead to low levels of ATP production and exposure to oxidative stress. Therefore, it is as important to selectively upregulate cytoprotective mechanisms such as the heat shock protein (HSP) response and expression of antioxidants as it is to shut down majority of ATP consuming processes in the cell. The objective of this study was to investigate another probable cytoprotective mechanism, anti-apoptosis during oxygen deprivation and recovery in the anoxia tolerant wood frog. In particular, relative protein expression levels of two important apoptotic regulator proteins, Bax and p-p53 (S46), and five anti-apoptotic/pro-survival proteins, Bcl-2, p-Bcl-2 (S70), Bcl-xL, x-IAP, and c-IAP in response to normoxic, 24 Hr anoxic exposure, and 4 Hr recovery stages were assessed in the liver and skeletal muscle using western immunoblotting. The results suggest a tissue-specific regulation of the anti-apoptotic pathway in the wood frog, where both liver and skeletal muscle shows an overall decrease in apoptosis and an increase in cell survival. This type of cytoprotective mechanism could be aimed at preserving the existing cellular components during long-term anoxia and oxygen recovery phases in the wood frog.


2016 ◽  
Vol 113 (32) ◽  
pp. 8991-8996 ◽  
Author(s):  
Sang Beom Kim ◽  
Jeremy C. Palmer ◽  
Pablo G. Debenedetti

The functional native states of globular proteins become unstable at low temperatures, resulting in cold unfolding and impairment of normal biological function. Fundamental understanding of this phenomenon is essential to rationalizing the evolution of freeze-tolerant organisms and developing improved strategies for long-term preservation of biological materials. We present fully atomistic simulations of cold denaturation of an α-helical protein, the widely studied Trp-cage miniprotein. In contrast to the significant destabilization of the folded structure at high temperatures, Trp-cage cold denatures at 210 K into a compact, partially folded state; major elements of the secondary structure, including the α-helix, are conserved, but the salt bridge between aspartic acid and arginine is lost. The stability of Trp-cage’s α-helix at low temperatures suggests a possible evolutionary explanation for the prevalence of such structures in antifreeze peptides produced by cold-weather species, such as Arctic char. Although the 310-helix is observed at cold conditions, its position is shifted toward Trp-cage’s C-terminus. This shift is accompanied by intrusion of water into Trp-cage’s interior and the hydration of buried hydrophobic residues. However, our calculations also show that the dominant contribution to the favorable energetics of low-temperature unfolding of Trp-cage comes from the hydration of hydrophilic residues.


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