Soil Bacterial and Fungal Richness and Network Exhibit Different Responses to Long-Term Throughfall Reduction in a Warm-Temperate Oak Forest

Prolonged drought results in serious ecological consequences in forest ecosystems, particularly for soil microbial communities. However, much is unknown about soil microbial communities in their response to long-term consecutive droughts in warm-temperate forests. Here, we conducted a 7-year manipulated throughfall reduction experiment (TFR) to examine the responses of bacterial and fungal communities in terms of richness and networks. Our results show that long-term TFR reduced bacterial, but not fungal, richness, with rare bacterial taxa being more sensitive to TFR than dominant taxa. The bacterial network under the TFR treatment featured a simpler network structure and fewer competitive links compared to the control, implying weakened interactions among bacterial species. Bacterial genes involved in xenobiotic biodegradation and metabolism, and lignin-degrading enzymes were enhanced under TFR treatment, which may be attributed to TFR-induced increases in fine root biomass and turnover. Our results indicate that soil bacterial communities are more responsive than fungi to long-term TFR in a warm-temperate oak forest, leading to potential consequences such as the degradation of recalcitrant organics in soil.

Download Full-text

Long-Term Drought and Warming Alter Soil Bacterial and Fungal Communities in an Upland Heathland

Ecosystems ◽

10.1007/s10021-021-00715-8 ◽

2021 ◽

Author(s):

Fiona M. Seaton ◽

Sabine Reinsch ◽

Tim Goodall ◽

Nicola White ◽

Davey L. Jones ◽

...

Keyword(s):

Climate Change ◽

Microbial Communities ◽

Soil Microbial Communities ◽

Fungal Communities ◽

Its2 Region ◽

Rrna Gene ◽

Summer Drought ◽

Soil Microbial ◽

Soil Bacterial

AbstractThe response of soil microbial communities to a changing climate will impact global biogeochemical cycles, potentially leading to positive and negative feedbacks. However, our understanding of how soil microbial communities respond to climate change and the implications of these changes for future soil function is limited. Here, we assess the response of soil bacterial and fungal communities to long-term experimental climate change in a heathland organo-mineral soil. We analysed microbial communities using Illumina sequencing of the 16S rRNA gene and ITS2 region at two depths, from plots undergoing 4 and 18 years of in situ summer drought or warming. We also assessed the colonisation of Calluna vulgaris roots by ericoid and dark septate endophytic (DSE) fungi using microscopy after 16 years of climate treatment. We found significant changes in both the bacterial and fungal communities in response to drought and warming, likely mediated by changes in soil pH and electrical conductivity. Changes in the microbial communities were more pronounced after a longer period of climate manipulation. Additionally, the subsoil communities of the long-term warmed plots became similar to the topsoil. Ericoid mycorrhizal colonisation decreased with depth while DSEs increased; however, these trends with depth were removed by warming. We largely ascribe the observed changes in microbial communities to shifts in plant cover and subsequent feedback on soil physicochemical properties, especially pH. Our results demonstrate the importance of considering changes in soil microbial responses to climate change across different soil depths and after extended periods of time.

Download Full-text

Different mechanisms underlying divergent responses of autotrophic and heterotrophic respiration to long-term throughfall reduction in a warm-temperate oak forest

Forest Ecosystems ◽

10.1186/s40663-021-00321-z ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Jinglei Zhang ◽

Shirong Liu ◽

Cuiju Liu ◽

Hui Wang ◽

Junwei Luan ◽

...

Keyword(s):

Soil Microorganisms ◽

Fine Root ◽

Fine Root Biomass ◽

Oak Forest ◽

Prolonged Drought ◽

Bacterial Richness ◽

The Impact ◽

Warm Temperate ◽

Throughfall Reduction

Abstract Background There are many studies on disentangling the responses of autotrophic (AR) and heterotrophic (HR) respiration components of soil respiration (SR) to long-term drought, but few studies have focused on the mechanisms underlying its responses. Methods To explore the impact of prolonged drought on AR and HR, we conducted the 2-year measurements on soil CO2 effluxes in the 7th and 8th year of manipulated throughfall reduction (TFR) in a warm-temperate oak forest. Results Our results showed long-term TFR decreased HR, which was positively related to bacterial richness. More importantly, some bacterial taxa such as Novosphingobium and norank Acidimicrobiia, and fungal Leptobacillium were identified as major drivers of HR. In contrast, long-term TFR increased AR due to the increased fine root biomass and production. The increased AR accompanied by decreased HR appeared to counteract each other, and subsequently resulted in the unchanged SR under the TFR. Conclusions Our study shows that HR and AR respond in the opposite directions to long-term TFR. Soil microorganisms and fine roots account for the respective mechanisms underlying the divergent responses of HR and AR to long-term TFR. This highlights the contrasting responses of AR and HR to prolonged drought should be taken into account when predicting soil CO2 effluxes under future droughts.

Download Full-text

Different mechanisms underlying divergent responses of autotrophic and heterotrophic respiration to long-term throughfall reduction in a warm-temperate oak forest

10.21203/rs.3.rs-333150/v1 ◽

2021 ◽

Author(s):

Jinglei Zhang ◽

Shirong Liu ◽

Cuiju Liu ◽

Hui Wang ◽

Junwei Luan ◽

...

Keyword(s):

Soil Microorganisms ◽

Fine Root ◽

Fine Root Biomass ◽

Oak Forest ◽

Prolonged Drought ◽

Bacterial Richness ◽

The Impact ◽

Warm Temperate ◽

Throughfall Reduction

Abstract Background: There are many studies on disentangling the responses of autotrophic (AR) and heterotrophic (HR) respiration components of soil respiration (SR) to long-term drought, but few studies have focused on the mechanisms underlying its responses.Methods: To explore the impact of prolonged drought on AR and HR, We conducted the 2-year measurements on soil CO2 effluxes in the 7th and 8th year of manipulated throughfall reduction (TFR) in a warm-temperate oak forest. Results: Our results showed long-term TFR decreased HR, which was positively related to bacterial richness. More importantly, some bacterial taxa such as Novosphingobium and norank Acidimicrobiia, and fungal Leptobacillium were identified as major drivers of HR. In contrast, long-term TFR increased AR due to the increased fine root biomass and production. The increased AR accompanied by decreased HR appeared to counteract each other, and subsequently resulted in the unchanged SR under the TFR. Conclusions: Our study shows that HR and AR respond in the opposite directions to long-term TFR. Soil microorganisms and fine roots account for the respective mechanisms underlying the divergent responses of HR and AR to long-term TFR. This highlights the contrasting responses of AR and HR to prolonged drought should be taken into account when predicting soil CO2 effluxes under future droughts.

Download Full-text

Faculty Opinions recommendation of Links between soil microbial communities and plant traits in a species-rich grassland under long-term climate change.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.727285484.793549366 ◽

2018 ◽

Author(s):

Carly Stevens

Keyword(s):

Climate Change ◽

Microbial Communities ◽

Plant Traits ◽

Soil Microbial Communities ◽

Soil Microbial

Download Full-text

Effects Of Pb, Cu, Sb, In and Ag Contamination on the Proliferation of Soil Bacterial Colonies, Soil Dehydrogenase Activity, and Phospholipid Fatty Acid Profiles of Soil Microbial Communities

Water Air & Soil Pollution ◽

10.1007/s11270-005-2254-x ◽

2005 ◽

Vol 164 (1-4) ◽

pp. 103-118 ◽

Cited By ~ 59

Author(s):

Tomoyoshi Murata ◽

Masami Kanao-Koshikawa ◽

Takejiro Takamatsu

Keyword(s):

Fatty Acid ◽

Microbial Communities ◽

Dehydrogenase Activity ◽

Phospholipid Fatty Acid ◽

Soil Microbial Communities ◽

Fatty Acid Profiles ◽

Soil Microbial ◽

Soil Bacterial ◽

Soil Dehydrogenase Activity

Download Full-text

Soil bacterial communities interact with silicon fraction transformation and promote rice yield after long-term straw return

10.5194/egusphere-egu21-760 ◽

2021 ◽

Author(s):

Alin Song ◽

Zimin Li ◽

Fenliang Fan

Keyword(s):

Microbial Community ◽

Rice Yield ◽

Soil Microbial Communities ◽

Nutrient Sources ◽

Soil Microbial ◽

Mn Oxide ◽

Straw Return ◽

Soil Bacterial ◽

Healthy Growth

<p>Returning crop straw into soil is an important practice to balance biogenic and bioavailable silicon (Si) pool in paddy, which is crucial for rice healthy growth. However, it remains elusive how straw return affects Si bioavailability, its uptake, and rice yield, owing to little knowledge about soil microbial communities responsible for straw degradation. Here, we investigated the change of soil Si fractions and microbial community in a 39-year-old paddy field amended by a long-term straw return. Results showed that rice straw-return significantly increased soil bioavailable Si and rice yield to from 29.9% to 61.6% and from 14.5% to 23.6%, respectively, compared to NPK fertilization alone. Straw return significantly altered soil microbial community abundance. Acidobacteria was positively and significantly related to amorphous Si, while Rokubacteria at the phylum level, Deltaproteobacteria and Holophagae at the class level were negatively and significantly related to organic matter adsorbed and Fe/Mn-oxide combined Si in soils. Redundancy analysis of their correlations further demonstrated that Si status significantly explained 12% of soil bacterial community variation. These findings suggest that soil bacteria community and diversity interact with Si mobility via altering its transformation, resulting in the balance of various nutrient sources to drive biological silicon cycle in agroecosystem.</p>

Download Full-text

Phosphorus Reduces Negative Effects of Nitrogen Addition on Soil Microbial Communities and Functions

Microorganisms ◽

10.3390/microorganisms8111828 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1828 ◽

Cited By ~ 1

Author(s):

Zongwei Xia ◽

Jingyi Yang ◽

Changpeng Sang ◽

Xu Wang ◽

Lifei Sun ◽

...

Keyword(s):

Microbial Communities ◽

Bacterial Diversity ◽

Soil Microbial Communities ◽

N Deposition ◽

N Fixation ◽

N Addition ◽

Soil Bacterial Diversity ◽

Soil Microbial ◽

Soil Bacterial ◽

P Addition

Increased soil nitrogen (N) from atmospheric N deposition could change microbial communities and functions. However, the underlying mechanisms and whether soil phosphorus (P) status are responsible for these changes still have not been well explained. Here, we investigated the effects of N and P additions on soil bacterial and fungal communities and predicted their functional compositions in a temperate forest. We found that N addition significantly decreased soil bacterial diversity in the organic (O) horizon, but tended to increase bacterial diversity in the mineral (A) horizon soil. P addition alone did not significantly change soil bacterial diversity but mitigated the negative effect of N addition on bacterial diversity in the O horizon. Neither N addition nor P addition significantly influenced soil fungal diversity. Changes in soil microbial community composition under N and P additions were mainly due to the shifts in soil pH and NO3− contents. N addition can affect bacterial functional potentials, such as ureolysis, N fixation, respiration, decomposition of organic matter processes, and fungal guilds, such as pathogen, saprotroph, and mycorrhizal fungi, by which more C probably was lost in O horizon soil under increased N deposition. However, P addition can alleviate or switch the effects of increased N deposition on the microbial functional potentials in O horizon soil and may even be a benefit for more C sequestration in A horizon soil. Our results highlight the different responses of microorganisms to N and P additions between O and A horizons and provides an important insight for predicting the changes in forest C storage status under increasing N deposition in the future.

Download Full-text