scholarly journals Evaluating the Effect of Lignocellulose-Derived Microbial Inhibitors on the Growth and Lactic Acid Production by Bacillus coagulans Azu-10

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 17 ◽  
Author(s):  
Mohamed Ali Abdel-Rahman ◽  
Saad El-Din Hassan ◽  
Amr Fouda ◽  
Ahmed A. Radwan ◽  
Mohammed G. Barghoth ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Production ◽  
Bacillus Coagulans ◽  
Potential Candidate ◽  
Xylose Consumption ◽  
Fermentation Conditions ◽  
Hydroxymethyl Furfural ◽  
Inhibitory Compounds ◽  
Pentose Sugar ◽  
Biomass Materials

Effective lactic acid (LA) production from lignocellulosic biomass materials is challenged by several limitations related to pentose sugar utilization, inhibitory compounds, and/or fermentation conditions. In this study, a newly isolated Bacillus coagulans strain Azu-10 was obtained and showed homofermentative LA production from xylose with optimal fermentation conditions at 50 °C and pH 7.0. Growth of strain Azu-10 and LA-fermentation efficiency were evaluated in the presence of various lignocellulose-derived inhibitors (furans, carboxylic acids, and phenols) at different concentrations. Furanic lignocellulosic-derived inhibitors were completely detoxified. The strain has exhibited high biomass, complete xylose consumption, and high LA production in the presence of 1.0–4.0 g/L furfural and 1.0–5.0 g/L of hydroxymethyl furfural, separately. Moreover, strain Azu-10 exhibited high LA production in the presence of 5.0–15.0 g/L acetic acid, 5.0 g/L of formic acid, and up to 7.0 g/L of levulinic acid, separately. Besides, for phenolic compounds, p-coumaric acid was most toxic at 1.0 g/L, while syringaldehyde or p-hydroxybenzaldehyde, and vanillin at 1.0 g/L did not inhibit LA fermentation. The present study provides an interesting potential candidate for the thermophilic LA fermentation from lignocellulose-derived substrates at the industrial biorefinery level.

scholarly journals A Process Study of Lactic Acid Production from Phragmites australis Straw by a Thermophilic Bacillus coagulans Strain under Non-Sterilized Conditions

Processes ◽  
2018 ◽  
Vol 6 (10) ◽  
pp. 175 ◽  
Author(s):  
Yuming Zhang ◽  
Mengran Li ◽  
Tian Nie ◽  
Zhihua Ni
Keyword(s):  
Lactic Acid ◽  
Phragmites Australis ◽  
Lactic Acid Production ◽  
Scale Up ◽  
Bacillus Coagulans ◽  
Dilute Acid Pretreatment ◽  
Integrated Process ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Process Study

Phragmites australis straw (PAS) is an abundant and renewable wetland lignocellulose. Bacillus coagulans IPE22 is a robust thermophilic strain with pentose-utilizing capability and excellent resistance to growth inhibitors. This work is focused on the process study of lactic acid (LA) production from P. australis lignocellulose which has not been attempted previously. By virtue of thermophilic feature of strain IPE22, two fermentation processes (i.e., separated process and integrated process), were developed and compared under non-sterilized conditions. The integrated process combined dilute-acid pretreatment, hemicellulosic hydrolysates fermentation, and cellulose utilization. Sugars derived from hemicellulosic hydrolysates and cellulose enzymatic hydrolysis were efficiently fermented to LA in a single vessel. Using the integrated process, 41.06 g LA was produced from 100 g dry PAS. The established integrated process results in great savings in terms of time and labor, and the fermentation process under non-sterilized conditions is easy to scale up for economical production of lactic acid from PAS.

scholarly journals Simultaneous saccharification and lactic acid fermentation of the cellulosic fraction of municipal solid waste using Bacillus smithii

2020 ◽  
Author(s):  
Micaela G. Chacón ◽  
Christopher Ibenegbu ◽  
David J. Leak
Keyword(s):  
Lactic Acid ◽  
Municipal Solid Waste ◽  
Solid Waste ◽  
Lactic Acid Production ◽  
Lactic Acid Fermentation ◽  
Potential Candidate ◽  
Acid Fermentation ◽  
Promising Candidate ◽  
Cellulosic Pulp ◽  
Simultaneous Saccharification

Abstract Objective A primary drawback to simultaneous saccharification and fermentation (SSF) processes is the incompatibility of the temperature and pH optima for the hydrolysis and fermentation steps—with the former working best at 50–55 °C and pH 4.5–5.5. Here, nine thermophilic Bacillus and Parageobacillus spp. were evaluated for growth and lactic acid fermentation at high temperature and low pH. The most promising candidate was then carried forward to demonstrate SSF using the cellulosic fraction from municipal solid waste (MSW) as a feedstock. Results B. smithii SA8Eth was identified as the most promising candidate and in a batch SSF maintained at 55 °C and pH 5.0, using a cellulase dose of 5 FPU/g glucan, it produced 5.1 g/L lactic acid from 2% (w/v) MSW cellulosic pulp in TSB media. Conclusion This work has both scientific and industrial relevance, as it evaluates a number of previously untrialled bacterial hosts for their compatibility with lignocellulosic SSF for lactic acid production and successfully identifies B. smithii as a potential candidate for such a process.

scholarly journals Production and Purification of l-lactic Acid in Lab and Pilot Scales Using Sweet Sorghum Juice

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 36 ◽  
Author(s):  
Agata Olszewska-Widdrat ◽  
Maria Alexandri ◽  
José Pablo López-Gómez ◽  
Roland Schneider ◽  
Michael Mandl ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Pilot Scale ◽  
Environmentally Benign ◽  
Downstream Process ◽  
Sweet Sorghum Juice ◽  
Ultra Filtration

Sweet sorghum juice (SSJ) was evaluated as fermentation substrate for the production of l-lactic acid. A thermophilic Bacillus coagulans isolate was selected for batch fermentations without the use of additional nutrients. The first batch of SSJ (Batch A) resulted on higher lactic acid concentration, yield and productivity with values of 78.75 g∙L−1, 0.78 g∙g−1 and 1.77 g∙L−1 h−1, respectively. Similar results were obtained when the process was transferred into the pilot scale (50 L), with corresponding values of 73 g∙L−1, 0.70 g∙g−1 and 1.47 g∙L−1 h−1. A complete downstream process scheme was developed in order to separate lactic acid from the fermentation components. Coarse and ultra-filtration were employed as preliminary separation steps. Mono- and bipolar electrodialysis, followed by chromatography and vacuum evaporation were subsequently carried out leading to a solution containing 905.8 g∙L−1 lactic acid, with an optical purity of 98.9%. The results of this study highlight the importance of the downstream process with respect to using SSJ for lactic acid production. The proposed downstream process constitutes a more environmentally benign approach to conventional precipitation methods.

scholarly journals Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate

2008 ◽  
Vol 78 (5) ◽  
pp. 751-758 ◽  
Author(s):  
Ronald H. W. Maas ◽  
Robert R. Bakker ◽  
Mickel L. A. Jansen ◽  
Diana Visser ◽  
Ed de Jong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Bacillus Coagulans ◽  
Fed Batch

Polymer grade l-lactic acid production from sugarcane bagasse hemicellulosic hydrolysate using Bacillus coagulans

2019 ◽  
Vol 6 ◽  
pp. 26-31 ◽  
Author(s):  
Regiane Alves de Oliveira ◽  
Roland Schneider ◽  
Carlos Eduardo Vaz Rossell ◽  
Rubens Maciel Filho ◽  
Joachim Venus
Keyword(s):  
Lactic Acid ◽  
Sugarcane Bagasse ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Bacillus Coagulans ◽  
Hemicellulosic Hydrolysate

Effect of Fermentation Conditions on L-Lactic Acid Production from Soybean Straw Hydrolysate

2015 ◽  
Vol 25 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Juan Wang ◽  
Qunhui Wang ◽  
Zhong Xu ◽  
Wenyu Zhang ◽  
Juan Xiang
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Fermentation Conditions ◽  
Soybean Straw

scholarly journals Major Role of NAD-Dependent Lactate Dehydrogenases in the Production of l-Lactic Acid with High Optical Purity by the Thermophile Bacillus coagulans

2014 ◽  
Vol 80 (23) ◽  
pp. 7134-7141 ◽  
Author(s):  
Limin Wang ◽  
Yumeng Cai ◽  
Lingfeng Zhu ◽  
Honglian Guo ◽  
Bo Yu
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Lactobacillus Delbrueckii ◽  
Content Type ◽  
High Optical Purity ◽  
Optically Pure

ABSTRACTBacillus coagulans2-6 is an excellent producer of optically purel-lactic acid. However, little is known about the mechanism of synthesis of the highly optically purel-lactic acid produced by this strain. Three enzymes responsible for lactic acid production—NAD-dependentl-lactate dehydrogenase (l-nLDH; encoded byldhL), NAD-dependentd-lactate dehydrogenase (d-nLDH; encoded byldhD), and glycolate oxidase (GOX)—were systematically investigated in order to study the relationship between these enzymes and the optical purity of lactic acid.Lactobacillus delbrueckiisubsp.bulgaricusDSM 20081 (ad-lactic acid producer) andLactobacillus plantarumsubsp.plantarumDSM 20174 (adl-lactic acid producer) were also examined in this study as comparative strains, in addition toB. coagulans. The specific activities of key enzymes for lactic acid production in the three strains were characterizedin vivoandin vitro, and the levels of transcription of theldhL,ldhD, and GOX genes during fermentation were also analyzed. The catalytic activities ofl-nLDH andd-nLDH were different inl-,d-, anddl-lactic acid producers. Onlyl-nLDH activity was detected inB. coagulans2-6 under native conditions, and the level of transcription ofldhLinB. coagulans2-6 was much higher than that ofldhDor the GOX gene at all growth phases. However, for the twoLactobacillusstrains used in this study,ldhDtranscription levels were higher than those ofldhL. The high catalytic efficiency ofl-nLDH toward pyruvate and the high transcription ratios ofldhLtoldhDandldhLto the GOX gene provide the key explanations for the high optical purity ofl-lactic acid produced byB. coagulans2-6.

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