scholarly journals Molecular Characterization and Expression of the LAP3 Gene and Its Association with Growth Traits in the Blood Clam Tegillarca granosa

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 54
Author(s):  
Hanhan Yao ◽  
Fuzhen Ren ◽  
Yongbo Bao ◽  
Yinghui Dong ◽  
Zhihua Lin
Keyword(s):  
Leucine Aminopeptidase ◽  
Catalytic Domain ◽  
Growth Traits ◽  
Genomic Structure ◽  
Shell Height ◽  
Proximal Promoter ◽  
Mizuhopecten Yessoensis ◽  
Tegillarca Granosa ◽  
Significant Difference ◽  
Blood Clam

Leucine aminopeptidase 3 (LAP3) is a metallopeptidase that cleaves N-terminal residues and is involved in protein maturation and degradation. In this study, we characterized the leucine aminopeptidase 3 (LAP3) gene from Tegillarca granosa (Tg-LAP3 for short), which appeared to consist of 15,731 nucleotides encoding 530 amino acids. We identified 12 introns and 13 exons in the Tg-LAP3 gene, suggesting a highly conserved genomic structure. The proximal promoter sequence consists of 1922 bps with a typical TATA box structure, which is the general structural characteristic of core promoters in eukaryotes. We found two functional domains in the Tg-LAP3 protein, including an N-terminal domain (41–174aa) and a peptidase_M17 catalytic domain (209–522aa). Multiple alignment showed that Tg-LAP3 shares 73.4% identity with LAP3 of Mizuhopecten yessoensis and 55.2–70.7% identity with LAP3 of other species. Quantitative analysis of Tg-LAP3 in embryos/larvae and adult tissues indicated that the highest expression occurred in eyebot larva, with limited expression in other stages; among tissues, the highest expression was found in the liver (p < 0.05). Association analysis found that three single-nucleotide polymorphisms (SNPs) (g.-488A > G, g.-1123C > T, and g.-1304C > A) in the proximal promoter were successfully typed, but there was no significant difference in growth traits (body weight, shell length, shell width, and shell height) among these genotypes. The results of our study demonstrate the functional roles of the Tg-LAP3 gene and provide valuable information for molecular marker-assisted selection (MAS) of the blood clam.

scholarly journals Dynamics of stock for yesso scallop Mizuhopecten yessoensis (Jay, 1856) in the northwestern Tatar Strait from the beginning of its fishery to nowadays

2021 ◽  
Vol 201 (3) ◽  
pp. 533-546
Author(s):  
P. A. Dulenina ◽  
A. A. Dulenin
Keyword(s):  
Shell Height ◽  
Mizuhopecten Yessoensis ◽  
Tatar Strait ◽  
Yesso Scallop ◽  
The Mean ◽  
Commercial Value ◽  
Northwestern Tatar Strait ◽  
Commercial Stock

Settlements of yesso scallop Mizuhopecten yessoensis in the northwestern Tatar Strait (within the waters of Khabarovsk Region) are considered on the data of scuba (< 20 m depth) and dredge (> 20 m depth) surveys conducted in 2001–2018. To 2018, only two settlements remain in this area from 9–12 ones in 2010–2014; both remained settlements have no commercial value. Trend to decreasing of the settlements density is shown: the mean density was 3.0 ind./m2 in 2001, 0.20 ind./m2 in 2010, and 0.0005 ind./m2 in 2018. Commercially valuable scallops with the shell height > 120 mm prevailed in the settlements (86–100 %), whereas juveniles were rare or absent (1.6 %, on average) in all years of surveys. CPUE decreased from 200 kg/diver/hour in 2001–2003 to 10 kg/diver/hour in 2018. These changes are obviously reasoned by overexploitation of the population using dredging and scuba gathering. Total commercial stock of yesso scallop dropped to the minimal value in 2018: 200 t, that was in 4.5 times lower than the established limit. Thus, 5 years ban is established for M. yessoensis landing in the area.

scholarly journals RESPONSE OF SOME WHEAT GROWTH TRAITS FOR FOR FOLIAR SPRAYING WITH HUMIC AND GLUTAMIC ACID

2019 ◽  
Vol 50 (6) ◽  
Author(s):  
Baqir & Zeboon
Keyword(s):  
Growth Rate ◽  
Humic Acid ◽  
Glutamic Acid ◽  
Growth Traits ◽  
Flag Leaf ◽  
Block Design ◽  
Dry Weight ◽  
Engineering Sciences ◽  
Significant Difference ◽  
Two Factors

A field experiment was conducted at the Agricultural Experiment Unit, College of Agriculture Engineering Sciences, University of Baghdad, during two winter seasons 2016-2017 and 2017-2018 to study the response of some growth traits for wheat Cv. Al forat to foliar spraying using with humic glutamic acid and acid. A factorial experiment was with in Randomized Complete Block Design applied three replications, it involved two factor ,first factor was glutamic acid with three concentrations (0,250,500) mg L-1, second factor was humic acid with three concentrations (0,1,2) ml L-1, have been sprayed at tillering and flowering stages. The results showed that all the studed growth traits (plant height, ,number of tillers, flag leaf area dry weight for plant , crop growth rate ,relative growth rate and biological yield) were affected  by spraying with humic acid and glutamic acid for two season concentration , 2ml -1 from humic acid was superior on most of studies traits , as for glutamic acid ,plants treated with concentrations 250 and 500 mg L-1 were produced the highest mean for studies traits but without significant difference between them in some traits .The interaction between two factors was significant on most studies growth traits .

scholarly journals Hormones and Heterosis in Hybrid Balsam Poplar (Populus balsamifera L.)

Forests ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 143 ◽  
Author(s):  
Yue Hu ◽  
Barb Thomas
Keyword(s):  
Growth Performance ◽  
Growth Traits ◽  
Block Design ◽  
Field Trials ◽  
Stem Volume ◽  
Hybrid Vigour ◽  
Populus Balsamifera ◽  
Balsam Poplar ◽  
Significant Difference ◽  
Cross Type

Balsam poplar (Populus balsamifera L.) is a transcontinental tree species in North America, making it an ideal species to study intra-specific hybrid vigour as a tool for increasing genetic gain in growth. We tested the hypothesis that intra-specific breeding of disparate populations of balsam poplar would lead to the expression of hybrid vigour and we determined the role of endogenous hormones linked to ecophysiological and growth performance. In September 2009, three field trials were established in Canada (two in Alberta (AB), i.e., Fields AB1 and AB2, and one in Quebec (QC), i.e., Field QC1) in conjunction with Alberta-Pacific Forest Industries Inc. and the Ministry of Forests, Wildlife and Parks, Quebec. Five male parents from each province as well as five female parents from QC and four female parents from AB were used for breeding intra-regional and inter-regional crosses. Based on a significant difference at year six for height and diameter, from the AB1 and AB2 field trials, the AB × QC cross-type was selected for further study. Cuttings from the AB × QC cross-type were grown in a randomized complete block design under near-optimal greenhouse conditions. Families were identified as slow- or fast-growing, and the relationship between hormone levels and growth performance of the genotypes within the families were examined. In late June, after 34 days of growth, internode tissue samples collected from each progeny were analyzed for gibberellic acids, indole-3-acetic acid, and abscisic acid content. Stem volume of two-month-old rooted cuttings, grown under optimal greenhouse conditions, was positively and significantly correlated with the photosynthetic rate, greenhouse growth, and stem volume of 8-year-old field-grown trees (Fields AB1 values: r = 0.629 and p = 0.012; AB2 values: r = 0.619 and p = 0.014, and QC1 values: r = 0.588 and p = 0.021, respectively). We determined that disparate and native populations of balsam poplar can be bred to produce superior progeny with enhanced stem growth traits.

The effects of sperm-egg ratios on polyspermy in the blood clam, Tegillarca granosa

2011 ◽  
Vol 43 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Yinghui Dong ◽  
Hanhan Yao ◽  
Zhihua Lin ◽  
Dongli Zhu
Keyword(s):  
Tegillarca Granosa ◽  
Blood Clam
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