scholarly journals Corn Bioethanol Side Streams: A Potential Sustainable Source of Fat-Soluble Bioactive Molecules for High-Value Applications

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1788
Author(s):  
Gabriella Di Lena ◽  
Jose Sanchez del Pulgar ◽  
Ginevra Lombardi Boccia ◽  
Irene Casini ◽  
Stefano Ferrari Nicoli
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Corn Oil ◽  
Lipid Fraction ◽  
Bioactive Molecules ◽  
Array Detector ◽  
Thin Stillage ◽  
Dry Grind ◽  
Liquid Chromatography Tandem Mass ◽  
One Year

This paper reports data from a characterization study conducted on the unsaponifiable lipid fraction of dry-grind corn bioethanol side streams. Phytosterols, squalene, tocopherols, tocotrienols, and carotenoids were quantified by High Performance Liquid Chromatography with Diode-Array Detector (HPLC-DAD) and Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) in different lots of post-fermentation corn oil and thin stillage collected from a bioethanol plant over a time-span of one year. Fat-soluble bioactives were present at high levels in corn oil, with a prevalence of plant sterols over tocols and squalene. Beta-sitosterol and sitostanol accounted altogether for more than 60% of total sterols. The carotenoid profile was that typical of corn, with lutein and zeaxanthin as the prevalent molecules. The unsaponifiable lipid fraction profile of thin stillage was qualitatively similar to that of post-fermentation corn oil but, in quantitative terms, the amounts of valuable biomolecules were much lower because of the very high dilution of this side stream. Results indicate that post-fermentation corn oil is a promising and sustainable source of health-promoting bioactive molecules. The concomitant presence of a variegate complex of bioactive molecules with high antioxidant potentialities and their potential multifaceted market applications as functional ingredients for food, nutraceutical, and cosmeceutical formulations, make the perspective of their recovery a promising strategy to create new bio-based value chains and maximize the sustainability of corn dry-grind bioethanol biorefineries.

scholarly journals Qualitative and Quantitative Analysis of C-glycosyl-flavones of Iris lactea Leaves by Liquid Chromatography/Tandem Mass Spectrometry

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3359 ◽  
Author(s):  
Dan Chen ◽  
Yu Meng ◽  
Yan Zhu ◽  
Gang Wu ◽  
Jun Yuan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Array Detector ◽  
Tandem Mass ◽  
Plant Resources ◽  
Plant Resource ◽  
Liquid Chromatography Tandem Mass ◽  
Main Components

Iris lactea Pall. var. chinensis (Fisch.) Koidz. is a traditional medicinal plant resource. To make full use of the I. lactea plant resources, constituents of I. lactea leaves were determined by high performance liquid chromatography (HPLC)-quadrupole time-of-flight tandem mass spectrometry and 22 C-glycosylflavones were identified or tentatively identified. Optimal extraction of I. lactea leaves was established via single factor investigations combined with response surface methodology. Then, HPLC coupled with a diode array detector was used to quantitatively analyze the six main components of 14 batches of I. lactea leaves grown in different areas. The results showed the C-glycosylflavones were the main components of I. lactea leaves, and the total contents of detected components were relatively stable for the majority of samples. These results provide a foundation for the development and utilization of I. lactea leaves.

scholarly journals Physicochemical stability of compounded midazolam capsules over a one-year storage period

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Pierre-Nicolas Boivin ◽  
Pauline Legendre ◽  
Anne-Claire Bonnaure ◽  
Marie-Antoinette Lester
Keyword(s):  
Clinical Trial ◽  
Liquid Chromatography ◽  
High Performance ◽  
Storage Period ◽  
Hepatic Metabolism ◽  
Hplc Method ◽  
Metabolic Capacity ◽  
Physicochemical Stability ◽  
Liquid Chromatography Tandem Mass ◽  
One Year

Abstract Objectives In patients suffering from chronic liver disease, the hepatic metabolism of drugs is perturbed and the metabolic capacity is difficult to assess. Midazolam could be used as a phenotypical probe to predict the metabolic capacity of CYP3A to adjust dosages of drug substrates of this cytochrome. In this context, a prospective clinical trial is going to be conducted in our institution and a hospital preparation of midazolam capsules suitable for the clinical trial was developed. The objective of the present work was to assess the physicochemical stability of the formulation over 12 months to set shelf life. Methods Three batches of 1 mg capsules were prepared using midazolam hydrochloride and microcrystalline cellulose as a diluent. The capsules were stored at ambient temperature and protected from light. To measure the evolution of the capsules content, a stability-indicating high-performance liquid chromatography (HPLC) method was developed with ultraviolet (UV) detection at 254 nm. Data were confirmed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method. Results After one year, midazolam hydrochloride content remained higher than 95% of the initial concentration in capsules. Conclusions The results show that 1 mg midazolam capsules are stable for 12 months at room temperature and under dark conditions.

scholarly journals Determination of phenmedipham and desmedipham in a commercial herbicide by high performance liquid chromatography

2004 ◽  
pp. 193-198 ◽  
Author(s):  
Eva Loncar ◽  
Petar Sekulic ◽  
Tijana Zeremski-Skoric ◽  
Radomir Malbasa ◽  
Ljiljana Kolarov
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phase Separation ◽  
High Performance ◽  
Reversed Phase ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
One Year

Betanal AM-11 is the herbicide that is using to control one-year old weeds with wide leafs in sugar beet fields. Active ingredients of the herbicide are phenmedipham and desmedipham. Commercial emulsifiable concentrate (EC) contains 80 g/L ( 10% of each active compound. We applied high performance liquid chromatography (HPLC) with diode array detector (DAD) at 254 nm for the determination of phenmedipham and desmedipham in commercial samples of Betanal AM-11. This method involves reversed-phase separation of the components on C-18 bonded silica with methanol-water (51+49, v/v) as the eluent. The procedure was highly selective and reproducible and can be successfully used in determining contents of phenmedipham and desmedipham on micro and macro levels.

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