scholarly journals Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel

Gels ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 3
Author(s):  
Cédric Decarpigny ◽  
Anne Ponchel ◽  
Eric Monflier ◽  
Rudina Bleta
Keyword(s):  
Catalytic Activity ◽  
High Efficiency ◽  
Catalytic Performance ◽  
Candida Antarctica ◽  
Pluronic F127 ◽  
Supramolecular Hydrogel ◽  
Lipase B ◽  
Interfacial Activation ◽  
Beneficial Effects ◽  
Highly Active

Surface modification plays a key role in the fabrication of highly active and stable enzymatic nanoreactors. In this study, we report for the first time the effect of various functional groups (epoxy, amine, trimethyl, and hexadecyl) on the catalytic performance of lipase B from Candida antarctica (CALB) incorporated within a monolithic supramolecular hydrogel with multiscale pore architecture. The supramolecular hydrogel formed by host-guest interactions between α-cyclodextrin (α-CD) and Pluronic F127 was first silicified to provide a hierarchically porous material whose surface was further modified with different organosilanes permitting both covalent anchoring and interfacial activation of CALB. The catalytic activity of nanoreactors was evaluated in the liquid phase cascade oxidation of 2,5-diformylfuran (DFF) to 2,5-furandicarboxylic acid (FDCA) under mild conditions. Results showed that high FDCA yields and high efficiency conversion of DFF could be correlated with the ability of epoxy and amine moieties to keep CALB attached to the carrier, while the trimethyl and hexadecyl groups could provide a suitable hydrophobic-hydrophilic interface for the interfacial activation of lipase. Cationic cross-linked β-CD was also evaluated as an enzyme-stabilizing agent and was found to provide beneficial effects in the operational stability of the biocatalyst. These supramolecular silicified hydrogel monoliths with hierarchical porosity may be used as promising nanoreactors to provide easier enzyme recovery in other biocatalytic continuous flow processes.

scholarly journals High-efficiency catalytic performance over mesoporous Ni/beta zeolite for the synthesis of quinoline from glycerol and aniline

RSC Advances ◽  
2017 ◽  
Vol 7 (16) ◽  
pp. 9551-9561 ◽  
Author(s):  
An Li ◽  
Chen Huang ◽  
Cai-Wu Luo ◽  
Wen-Jun Yi ◽  
Zi-Sheng Chao
Keyword(s):  
Catalytic Activity ◽  
Vapor Phase ◽  
High Efficiency ◽  
Zeolite Catalyst ◽  
Catalytic Performance ◽  
Beta Zeolite ◽  
Phase Process

Quinoline was synthesized via the typical Skraup approach with a vapor-phase process. The mesoporous Ni/beta zeolite catalyst exhibited high-efficiency catalytic activity and an enhanced ability of anti-deactivation.

Candida antarctica lipase B-catalyzed regioselective deacylation of dihydroxybenzenes acylated at both phenolic hydroxy groups

2016 ◽  
Vol 94 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Toshifumi Miyazawa ◽  
Manabu Hamada ◽  
Ryohei Morimoto
Keyword(s):  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Primary Alcohols ◽  
Lipase B ◽  
Highly Active ◽  
Direct Acylation ◽  
Acyl Acceptors ◽  
Hydroxy Groups ◽  
Phenolic Hydroxy

Candida antarctica lipase B proved to be highly active in the deacylation of substituted hydroquinones and resorcinols acylated at both phenolic hydroxy groups. The deacylation reactions were much faster than the corresponding direct acylations of these dihydroxybenzenes catalyzed by the same lipase. More importantly, they took place generally in a markedly regioselective manner: the acyloxy group remote from the substituent was preferentially cleaved. The main or exclusive products obtained were the regioisomers of those produced through the direct acylation of the dihydroxybenzenes. In the case of alkyl-substituted hydroquinone derivatives, the regioselectivity increased with an increase in the bulk of the substituent. In the case of 4-substituted diacylated resorcinols, the 3-O-monoacyl derivatives were obtained generally as the sole products. Quite interestingly, some secondary alcohols proved to act as better acyl acceptors than the corresponding primary alcohols in these enzymatic deacylations.

Magnetic Nanoparticles Supported Acidic Ionic Liquid as a Highly Active and Reusable Catalyst for Esterification

2016 ◽  
Vol 852 ◽  
pp. 485-488 ◽  
Author(s):  
Qiang Zhang ◽  
Xin Zhao ◽  
Xue Hua Zhu ◽  
Ji Hang Li
Keyword(s):  
Ionic Liquid ◽  
Catalytic Activity ◽  
Magnetic Nanoparticles ◽  
Catalytic Performance ◽  
Significant Loss ◽  
Reusable Catalyst ◽  
Hydrogen Sulfate ◽  
External Magnet ◽  
Acidic Ionic Liquid ◽  
Highly Active

A magnetic nanoparticles supported dual acidic ionic liquid catalyst was prepared via anchoring 3-sulfobutyl-1-(3-propyltriethoxysilane) imidazolium hydrogen sulfate onto the surface of silica-coated Fe3O4 nanoparticles. And this novel supported acidic ionic liquid catalyst showed good catalytic performance in esterification. More importantly, the catalyst could be easily recovered by an external magnet and reused six times without significant loss of catalytic activity.

scholarly journals Interfacial Activation of Candida antarctica Lipase B: Combined Evidence from Experiment and Simulation

Biochemistry ◽  
2015 ◽  
Vol 54 (38) ◽  
pp. 5969-5979 ◽  
Author(s):  
Themistoklis Zisis ◽  
Peter L. Freddolino ◽  
Petri Turunen ◽  
Muriel C. F. van Teeseling ◽  
Alan E. Rowan ◽  
...  
Keyword(s):  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Interfacial Activation ◽  
Experiment And Simulation

scholarly journals PTFE-Carbon Nanotubes and Lipase B from Candida antarctica—Long-Lasting Marriage for Ultra-Fast and Fully Selective Synthesis of Levulinate Esters

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1518
Author(s):  
Anna Szelwicka ◽  
Agnieszka Siewniak ◽  
Anna Kolanowska ◽  
Sławomir Boncel ◽  
Anna Chrobok
Keyword(s):  
Carbon Nanotubes ◽  
Levulinic Acid ◽  
Active Phase ◽  
Candida Antarctica ◽  
High Yield ◽  
Lipase B ◽  
Multi Wall Carbon Nanotubes ◽  
Interfacial Activation ◽  
Fischer Esterification ◽  
Heterogeneous Biocatalyst

An effective method for levulinic acid esters synthesis by the enzymatic Fischer esterification of levulinic acid using a lipase B from Candida antarctica (CALB) immobilized on the advanced material consisting of multi-wall carbon nanotubes (MWCNTs) and a hydrophobic polymer—polytetrafluoroethylene (Teflon, PTFE)—as a heterogeneous biocatalyst, was developed. An active phase of the biocatalyst was obtained by immobilization via interfacial activation on the surface of the hybrid material MWCNTs/PTFE (immobilization yield: 6%, activity of CALB: 5000 U∙L∙kg−1, enzyme loading: 22.5 wt.%). The catalytic activity of the obtained biocatalyst and the effects of the selected reaction parameters, including the agitation speed, the amount of PTFE in the CALB/MWCNT-PTFE biocatalyst, the amount of CALB/MWCNT-PTFE, the type of organic solvent, n-butanol excess, were tested in the esterification of levulinic acid by n-butanol. The results showed that the use of a two-fold excess of levulinic acid to n-butanol, 22.5 wt.% of CALB on MWCNT-PTFE (0.10 wt.%) and cyclohexane as a solvent at 20 °C allowed one to obtain n-butyl levulinate with a high yield (99%) and selectivity (>99%) after 45 min. The catalyst retained its activity and stability after three cycles, and then started to lose activity until dropping to a 69% yield of ester in the sixth reaction run. The presented method has opened the new possibilities for environmentally friendly synthesis of levulinate esters.

Highly Active and Selective PtSnM1/γ-Al2O3 Catalyst for Direct Propane Dehydrogenation

2021 ◽  
Vol 43 (3) ◽  
pp. 342-342
Author(s):  
Arshid M Ali Arshid M Ali ◽  
Abdulrahim A Zahrani Abdulrahim A Zahrani ◽  
Muhammad A Daous Muhammad A Daous ◽  
Muhammad Umar Seetharamulu Podila and Lachezar A Petrov Muhammad Umar Seetharamulu Podila and Lachezar A Petrov
Keyword(s):  
Catalytic Activity ◽  
Coke Formation ◽  
Protective Layer ◽  
Catalytic Performance ◽  
Active Species ◽  
Propane Dehydrogenation ◽  
Propane Conversion ◽  
Feed Composition ◽  
Highly Active ◽  
Al2o3 Catalyst

This study is aimed to understand the role of alkaline earth elements (AEE) to the catalytic performance of PtSnM1/γ-Al2O3catalystfor the direct propane dehydrogenation (where M1 = Mg, Ca, Sr, Ba). All the catalysts were prepared by using wet impregnation.The overall catalytic performance of all the catalysts was studied at different reaction temperatures, feed composition ratios and GHSV. The best operating reaction conditions were575and#186;C, feed composition ratio of C3H8:H2:N2 = 1.0:0.5:5.5 and GHSV of 3800h-1. An optimal addition of “Ca” to PtSn//γ-Al2O3 catalyst, enhanced the catalytic activity of PtSnM1/γ-Al2O3 catalyst in comparison to other studied AEE. This catalyst had shown the highest propane conversion (~ 55.8 %) with 95.7 % propylene selectivity and least coke formation (7.11 mg.g-1h-1). In general, the increased catalytic activity of PtSnM1/γ-Al2O3 is attributed to the reduced coking extent during the reaction. In addition, the enhanced thermal stability of the PtSnCa/γ-Al2O3catalystis because of the protective layer betweenγ-Al2O3 and active metal, which allows the formation of active species such as PtSn, PtCa2 and Pt2Al phases?

Significantly increased catalytic activity of Candida antarctica lipase B for the resolution of cis-(±)-dimethyl 1-acetylpiperidine-2,3-dicarboxylate

2018 ◽  
Vol 8 (18) ◽  
pp. 4718-4725 ◽  
Author(s):  
Jiang-Wei Shen ◽  
Jia-Mei Qi ◽  
Xiao-Jian Zhang ◽  
Zhi-Qiang Liu ◽  
Yu-Guo Zheng
Keyword(s):  
Catalytic Activity ◽  
Binding Pocket ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Rational Engineering ◽  
Engineering Approach

Structure-based semi-rational engineering approach was applied to alter the binding pocket and substrate channel for enhancing the activity of CALB towards moxifloxacin chiral intermediate.

Sign in / Sign up

Export Citation Format

Share Document