scholarly journals Genomic Comparison of Insect Gut Symbionts from Divergent Burkholderia Subclades

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 744
Author(s):  
Kazutaka Takeshita ◽  
Yoshitomo Kikuchi
Keyword(s):  
Genome Comparison ◽  
Stink Bug ◽  
Stink Bugs ◽  
Monophyletic Clade ◽  
Genomic Comparison ◽  
Genomic Features ◽  
The Family ◽  
Gut Symbionts ◽  
Whole Genome Comparison ◽  
Insect Gut

Stink bugs of the superfamilies Coreoidea and Lygaeoidea establish gut symbioses with environmentally acquired bacteria of the genus Burkholderia sensu lato. In the genus Burkholderia, the stink bug-associated strains form a monophyletic clade, named stink bug-associated beneficial and environmental (SBE) clade (or Caballeronia). Recently, we revealed that members of the family Largidae of the superfamily Pyrrhocoroidea are associated with Burkholderia but not specifically with the SBE Burkholderia; largid bugs harbor symbionts that belong to a clade of plant-associated group of Burkholderia, called plant-associated beneficial and environmental (PBE) clade (or Paraburkholderia). To understand the genomic features of Burkholderia symbionts of stink bugs, we isolated two symbiotic Burkholderia strains from a bordered plant bug Physopellta gutta (Pyrrhocoroidea: Largidae) and determined their complete genomes. The genome sizes of the insect-associated PBE (iPBE) are 9.5 Mb and 11.2 Mb, both of which are larger than the genomes of the SBE Burkholderia symbionts. A whole-genome comparison between two iPBE symbionts and three SBE symbionts highlighted that all previously reported symbiosis factors are shared and that 282 genes are specifically conserved in the five stink bug symbionts, over one-third of which have unknown function. Among the symbiont-specific genes, about 40 genes formed a cluster in all five symbionts; this suggests a “symbiotic island” in the genome of stink bug-associated Burkholderia.

scholarly journals A gene based bacterial whole genome comparison toolkit

2019 ◽  
Vol 26 (1) ◽  
pp. 36
Author(s):  
Luciano Antonio Digiampietri ◽  
Vivian Mayumi Yamassaki Pereira ◽  
Geraldo José Santos-Júnior ◽  
Giovani Sousa-Leite ◽  
Priscilla Koch Wagner ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genome Comparison ◽  
Local Alignment ◽  
Whole Genome ◽  
Rrna Processing ◽  
Sequence Alignments ◽  
Genomic Features ◽  
Whole Genomes ◽  
Study Case ◽  
Whole Genome Comparison

Most of the computational biology analysis is made comparing genomic features. The nucleotide and amino acid sequence alignments are frequently used in gene function identification and genome comparison. Despite its widespread use, there are limitations in their analysis capabilities that need to be considered but are often overlooked or unknown by many researchers. This paper presents a gene based whole genome comparison toolkit which can be used not only as an alternative and more robust way to compare a set of whole genomes, but, also, to understand the tradeoff of the use of sequence local alignment in this kind of comparison. A study case was performed considering fifteen whole genomes of the Xanthomonas genus. The results were compared with the 16S rRNA-processing protein RimM phylogeny and some thresholds for the use of sequence alignments in this kind of analysis were discussed.

scholarly journals Inhibitory Copulation Effect of Vibrational Rival Female Signals of Three Stink Bug Species as a Tool for Mating Disruption

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 177
Author(s):  
Aline Moreira Dias ◽  
Miguel Borges ◽  
Maria Carolina Blassioli Moraes ◽  
Matheus Lorran Figueira Coelho ◽  
Andrej Čokl ◽  
...  
Keyword(s):  
Pest Management ◽  
Mating Behavior ◽  
Mating Disruption ◽  
Management Strategies ◽  
Stink Bug ◽  
Stink Bugs ◽  
Vibratory Communication ◽  
Communication Signals ◽  
Long Duration ◽  
Bean Plants

Stink bugs are major pests in diverse crops around the world. Pest management strategies based on insect behavioral manipulation could help to develop biorational management strategies of stink bugs. Insect mating disruption using vibratory signals is an approach with high potential for pest management. The objective of this work was to investigate the effect of conspecific female rival signals on the mating behavior and copulation of three stink bug species to establish their potential for mating disruption. Previously recorded female rival signals were played back to bean plants where pairs of the Neotropical brown stink bug, Euschistus heros, and two green stink bugs, Chinavia ubica and Chinavia impicticornis were placed. Vibratory communication and mating behavior were recorded for each pair throughout the experimental time (20 min). Female rival signals show a disrupting effect on the reproductive behavior of three conspecific investigated stink bug species. This effect was more clearly expressed in E. heros and C. ubica than in C. impicticornis. The likelihood of copulating in pairs placed on control plants, without rival signals, increased 29.41 times in E. heros, 4.6 times in C. ubica and 1.71 times in C. impicticornis. However, in the last case, the effect of female rivalry signals in copulation was not significant. The effect of mating disruption of female rival signals of the three stink bug species may originate from the observed reduction in specific vibratory communication signals emitted, which influences the duet formation and further development of different phases of mating behavior. Our results suggest that female rival signals have potential for application in manipulation and disruption of mating behavior of stink bugs. Further work needs to focus on the effects of female rival signals used in long duration experiments and also their interactions with chemical communication of stink bugs.

Comparative analysis of insect gut symbionts for composition–function relationships and biofuel application potential

Biofuels ◽  
2011 ◽  
Vol 2 (5) ◽  
pp. 529-544 ◽  
Author(s):  
Weibing Shi ◽  
Ugur Uzuner ◽  
Lingxia Huang ◽  
Palmy R Jesudhasan ◽  
Suresh D Pillai ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Application Potential ◽  
Gut Symbionts ◽  
Insect Gut

scholarly journals Whole genome comparison of donor and cloned dogs

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Hak-Min Kim ◽  
Yun Sung Cho ◽  
Hyunmin Kim ◽  
Sungwoong Jho ◽  
Bongjun Son ◽  
...  
Keyword(s):  
Genome Comparison ◽  
Whole Genome ◽  
Whole Genome Comparison

scholarly journals Kahawai Phylogeny and Phylogenetics:  A Genetic Investigation into Commercial and Recreational Fisheries Management and Practice

2021 ◽  
Author(s):  
◽  
Brenton Hodgson
Keyword(s):  
New Zealand ◽  
Common Ancestor ◽  
Coastal Communities ◽  
Commercial Fishing ◽  
Recreational Fisheries ◽  
Monophyletic Clade ◽  
Genetic Investigation ◽  
Fish Stocks ◽  
Modern Times ◽  
The Family

<p>Globally, commercially exploited fish species are coming under more and more pressure as the population of humans grow. Protein from the sea has traditionally been available to coastal communities throughout history. In modern times however, traditional artisanal fisheries have been replaced by commercial fishing industries. It is estimated by some authorities that these modern fisheries have led to decreases in pre-exploitation biomass of desirable species of up to 90%. As desirable species decline, secondary species become more valuable and subject to exploitation. An issue with this exploitation is that management decisions of fish stocks are often based on political or commercial concerns rather than sound science focussed on preserving stocks, and ultimately, fishing industries. To investigate phylogenetic and phylogeographic relationships of fish, kahawai (Arripis trutta) was used as a proxy species. A. trutta is one of only four members of the genus Arripis, which in turn is the sole member of the family Arripidae. It was found that a single, highly connected population of A. trutta inhabit New Zealand waters, and approximately 15 migrants per generation make the journey between New Zealand and Australia, genetically linking these populations. A phylogeny of A. trutta was resolved using mitochondrial DNA, and while COX1 data supported the hypothesis that A. trutta forms a monophyletic clade within the Stromateoids (medusa fish, squaretails and drift fish) and the Scombrids (tuna, mackerel and their allies) suggesting a common ancestor, other data collected during the investigation does not support this hypothesis.</p>

scholarly journals Pentatomids associated with blackberry

2014 ◽  
Vol 38 (3) ◽  
pp. 256-261
Author(s):  
Mauricio Paulo Batistella Pasini ◽  
Alessandro Dal'Col Lúcio
Keyword(s):  
Rio Grande ◽  
Nezara Viridula ◽  
Brown Spot ◽  
Stink Bug ◽  
Stink Bugs ◽  
Rio Grande Do Sul ◽  
Dark Brown Spot ◽  
Euschistus Heros ◽  
Santa Maria ◽  
Piezodorus Guildinii

Information concerning the presence of stink bugs in blackberry (Rubus spp.) in Brazil is sparse. This study aimed to identify the stink bug species associated with blackberry, to establish the daily dynamics and evaluate the fruits damage. The experiment was conducted in Santa Maria, Rio Grande do Sul State, Brazil, in blackberry orchard. Presence and evaluations of stink bugs were done weekly through visual and sweeping samplings at different day times. Five species of pentatomids were identified: Piezodorus guildinii, Nezara viridula, Euschistus heros, Dichelops furcatus and Edessa meditabunda. The bugs attack the drupelets producing a dark brown spot and wrinkled berries.

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