scholarly journals Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 885
Author(s):  
Takafumi Shimizu ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Shunsuke Watanabe ◽  
Mitsunori Seo
Keyword(s):  
Abscisic Acid ◽  
Plant Hormone ◽  
Leaf Surface ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Cell Types ◽  
Acid Transport ◽  
Wild Type ◽  
Vascular Tissues ◽  
Abscisic Acid Transport

The plant hormone abscisic acid (ABA) is actively synthesized in vascular tissues and transported to guard cells to promote stomatal closure. Although several transmembrane ABA transporters have been identified, how the movement of ABA within plants is regulated is not fully understood. In this study, we determined that Arabidopsis NPF4.6, previously identified as an ABA transporter expressed in vascular tissues, is also present in guard cells and positively regulates stomatal closure in leaves. We also found that mutants defective in NPF5.1 had a higher leaf surface temperature compared to the wild type. Additionally, NPF5.1 mediated cellular ABA uptake when expressed in a heterologous yeast system. Promoter activities of NPF5.1 were detected in several leaf cell types. Taken together, these observations indicate that NPF5.1 negatively regulates stomatal closure by regulating the amount of ABA that can be transported from vascular tissues to guard cells.

scholarly journals A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1247-1255 ◽  
Author(s):  
Eiji Nambara ◽  
Masaharu Suzuki ◽  
Suzanne Abrams ◽  
Donald R McCarty ◽  
Yuji Kamiya ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Growth And Development ◽  
Plant Hormone ◽  
The Other ◽  
Aba Signaling ◽  
Wild Type ◽  
Plant Growth And Development ◽  
Diverse Range ◽  
Abscisic Acid Signaling ◽  
Aba Insensitive

Abstract The plant hormone abscisic acid (ABA) controls many aspects of plant growth and development under a diverse range of environmental conditions. To identify genes functioning in ABA signaling, we have carried out a screen for mutants that takes advantage of the ability of wild-type Arabidopsis seeds to respond to (−)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA. The premise of the screen was to identify mutations that preferentially alter their germination response in the presence of one stereoisomer vs. the other. Twenty-six mutants were identified and genetic analysis on 23 lines defines two new loci, designated CHOTTO1 and CHOTTO2, and a collection of new mutant alleles of the ABA-insensitive genes, ABI3, ABI4, and ABI5. The abi5 alleles are less sensitive to (+)-ABA than to (−)-ABA. In contrast, the abi3 alleles exhibit a variety of differences in response to the ABA isomers. Genetic and molecular analysis of these alleles suggests that the ABI3 transcription factor may perceive multiple ABA signals.

Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells

2021 ◽  
Author(s):  
Md Tahjib-Ul-Arif ◽  
Shintaro Munemasa ◽  
Toshiyuki Nakamura ◽  
Yoshimasa Nakamura ◽  
Yoshiyuki Murata
Keyword(s):  
Plasma Membrane ◽  
Stomatal Closure ◽  
Anion Channel ◽  
Guard Cells ◽  
Cytosolic Calcium ◽  
Peak Height ◽  
Extracellular Calcium ◽  
Wild Type ◽  
Anion Channels ◽  
In The Wild

Abstract Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild-type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild-type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.

scholarly journals PYR/PYL/RCAR Abscisic Acid Receptors Regulate Root Cell hydraulic Conductivity through Activating Aquaporin expression

2018 ◽  
Vol 10 (3) ◽  
pp. 7
Author(s):  
Xiaolin Wang ◽  
Ningning Zhang ◽  
Jiakun Yan
Keyword(s):  
Abscisic Acid ◽  
Hydraulic Conductivity ◽  
Physical Environment ◽  
Stomatal Closure ◽  
Root Cell ◽  
Physiological Adaptation ◽  
Pressure Probe ◽  
Wild Type ◽  
Long Time ◽  
Pcr Technology

With a varied physical environment, plants have developed specific mechanisms that allow them to rapidly perceive and respond to stresses in the environment. As one of many pathways conserved in a long time evolution, the abscisic acid (ABA) signaling pathway has been identified as a central regulator to control gene expression and generate physiological adaptation to various stressful conditions in plant. As abscisic acid receptors, PYR/PYL/RCARs have been proved to regulate stomatal closure. Here we detected the root cell hydraulic conductivity and the aquaporin expression under treatment of exogenous ABA in wild-type and pyr1/pyl1/pyl2/pyl4 mutant Arabidopsis using cell pressure probe and qRT-PCR technology. The results showed that PYR/PYL/RCAR receptors regulate root cell hydraulic conductivity through activating aquaporin expression. Lastly, we discussed how plants maintain water balance combined with the data obtained from published articles.

scholarly journals Abscisic Acid Transport in Human Erythrocytes

2015 ◽  
Vol 290 (21) ◽  
pp. 13042-13052 ◽  
Author(s):  
Tiziana Vigliarolo ◽  
Lucrezia Guida ◽  
Enrico Millo ◽  
Chiara Fresia ◽  
Emilia Turco ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Human Erythrocytes ◽  
Acid Transport ◽  
Abscisic Acid Transport

Alteration of anion channel kinetics in wild-type and abi1-1 transgenic Nicotiana benthamiana guard cells by abscisic acid

1997 ◽  
Vol 12 (1) ◽  
pp. 203-213 ◽  
Author(s):  
Alexander Grabov ◽  
Jeffrey Leung ◽  
Jerome Giraudat ◽  
Michael R. Blatt
Keyword(s):  
Abscisic Acid ◽  
Nicotiana Benthamiana ◽  
Anion Channel ◽  
Guard Cells ◽  
Channel Kinetics ◽  
Wild Type

scholarly journals Downregulating VAC14 in Guard Cells Causes Drought Hypersensitivity by Inhibiting Stomatal Closure

2020 ◽  
Vol 11 ◽  
Author(s):  
Zong-Qi Wang ◽  
Qi Liu ◽  
Ju-Hua Wu ◽  
Juan Li ◽  
Jun-Min He ◽  
...  
Keyword(s):  
Stomatal Closure ◽  
Guard Cells ◽  
Land Plant ◽  
Scaffolding Protein ◽  
Stomatal Movement ◽  
Wild Type ◽  
Physiological Factors ◽  
Surrounding Environment ◽  
Intracellular Activities

Stomata are a key land plant innovation that permit the regulation of gaseous exchanges between the plant interior and the surrounding environment. By opening or closing, stomata regulate transpiration of water though the plant; and these actions are coordinated with acquisition of CO2 for photosynthesis. Stomatal movement is controlled by various environmental and physiological factors and associates with multiple intracellular activities, among which the dynamic remodeling of vacuoles plays a crucial role. Phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2] is critical for dynamic remodeling of vacuoles. Its production requires a PI(3,5)P2-metabolizing complex consisting of FAB1/PIKfyve kinases, SAC phosphatases, and the scaffolding protein VAC14. Although genetic or pharmacological downregulation of PI(3,5)P2 causes hyposensitivity to ABA-induced stomatal closure, whether the effect of PI(3,5)P2 on stomatal movement is cell-autonomous and the physiological consequences of its reduction were unclear. We report that downregulating Arabidopsis VAC14 specifically in guard cells by artificial microRNAs (amiR-VAC14) results in enlarged guard cells and hyposensitivity to ABA- and dark-induced stomatal closure. Vacuolar fission during stomatal closure is compromised by downregulating VAC14 in guard cells. Exogenous application of PI(3,5)P2 rescued the amiR-VAC14 phenotype whereas PI(3,5)P2 inhibitor YM201636 caused wild-type plants to have inhibited stomatal closure. We further show that downregulating VAC14 specifically in guard cells impairs drought tolerance, suggestive of a key role of guard cell-produced PI(3,5)P2 in plant fitness.

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