Post-Harvest Non-Conventional and Traditional Methods to Control Cadophora luteo-olivacea: Skin Pitting Agent of Actinidia chinensis var. deliciosa (A. Chev.)

Cadophora luteo-olivacea represents a critical problem for kiwifruit in the post-harvest phase, mainly for its little note epidemiology. The study presented some results about the possibility of preserving kiwifruit from skin pitting symptoms using alternative methods to fungicides. By in vitro assays, antagonist mechanisms of action against pathogen isolates were tested. Trichoderma harzianum (Th1) showed the highest inhibitory activity against C. luteo-olivacea isolates by volatile, non-volatile, and by dual culture assay, displaying an inhibition respectively by 90%, 70.6%, and 78.8%, and with respect to Aureobasidium pullulans (L1 and L8) by 23.3% and 25.8%, 50% and 34.7%, and 22.5% and 23.6%, respectively. Further, the sensitivity on CFU and mycelial growth of C. luteo-olivacea isolates to fludioxonil, and CaCl2 was tested, displaying interesting EC50 values (0.36 and 0.92 g L−1, 22.5 g L−1, respectively). The effect of Brassica nigra defatted meal was tested as biofumigation assays and through FT-IR (Fourier-Transform Infrared) spectroscopy. The above-mentioned treatments were applied in vivo to evaluate their efficacy on kiwifruits. Our data demonstrated that alternative solutions could be considered to control postharvest pathogens such as C. luteo-olivacea.

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In vitro and in vivo Assay for Assessment of the Biological Control Potential of Streptomyces sp. KT

Journal of Plant Studies ◽

10.5539/jps.v7n1p10 ◽

2017 ◽

Vol 7 (1) ◽

pp. 10

Author(s):

Tatsuya Ohike ◽

Minori Maeda ◽

Tetsuya Matsukawa ◽

Masahiro Okanami ◽

Shin’ichiro Kajiyama ◽

...

Keyword(s):

Biological Control ◽

Suppressive Effect ◽

Phytopathogenic Fungi ◽

Dual Culture ◽

Biological Control Agents ◽

Bipolaris Oryzae ◽

Biological Control Potential ◽

Dual Culture Assay

Rhizoctonia solani is fungal plant pathogen that infects many different host plants. Recently, biological control agents that are friendly to the environment and ecosystems have attracted much attention as an alternative to the use of chemical fungicide which have been used worldwide to control soil borne pathogens including R. solani. In this study, 53 strains of actinomycetes isolated from environmental soils, and antifungal activities of them were assessed by the dual culture assay. Strain KT showed strong inhibitory activities against 8 phytopathogenic fungi. A great suppressive effect on R. solani growth was observed in the inoculation test of plants using cucumber and chin-geng-sai. In addition, infection of Bipolaris oryzae also could be suppressed in the detached leaf assay using oats. As a result of genetic analysis, it was shown that KT was a species closely related to Streptomyces lavenduligriseus NRRL B-3173T. However, as far as we know, there is no report for biological control agents using S. lavenduligriseus. This study suggests that the strain KT may useful as biological control agents to suppress various crop diseases.

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Efficacy, toxicity, and lethality of plants with potential anthelmintic activity in small ruminants in Brazil

Revista Brasileira de Saúde e Produção Animal ◽

10.1590/s1519-9940200232019 ◽

2019 ◽

Vol 20 ◽

Author(s):

Vanessa Daniele Mottin ◽

Jurandir Ferreira da Cruz ◽

Milton Resende Teixeira Neto ◽

Gabriele Marisco ◽

Jennifer Souza Figueredo ◽

...

Keyword(s):

Small Ruminants ◽

Anthelmintic Activity ◽

Production Costs ◽

Alternative Methods ◽

In Vitro Assays ◽

Toxicity Tests ◽

Action Preparation ◽

Chemical Inputs

SUMMARY The use of medicinal plants as a therapeutic method in the control of diseases has been increasing in animal production. In the case of small ruminants, the endoparasitic disease is a major concern, since they are considered the greater sanitary problem, especially when considering the phenomenon of parasite resistance to the medicines used. Therefore, the development of alternative methods of endoparasitary control in goats and sheep has become a pressing need. The use of plants to control endoparasites can reduce the use of chemical inputs, making more environmentally sustainable livestock, minimizing the occurrence of parasitic resistance to conventional medicines and contributing to the reduction of production costs. In this sense, several studies have been carried out in order to evaluate the in vitro and in vivo activity of plants with anthelmintic potential. However, despite the potentiality found in many studies, the results are sometimes contradictory or do not replicate in vivo the same efficacy obtained in the in vitro assays. Another relevant aspect is the low utilization of the lethality and toxicity tests, which are indispensable so that the knowledge can be spread and applied by the producers in their herds. Thus, this review aims to provide the results from studies carried out in Brazil with extract of plants with potential for control of parasitic disease in goats and sheep, describing the main evaluated plants species, mechanisms of action, preparation forms and tests of efficacy, toxicity, and lethality.

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Biocontrol of Pythium Damping-Off on Pepper (Capsicum Annuum) with Selected Fungal and Rhizobacterial Agents

International Journal of Phytopathology ◽

10.33687/phytopath.009.01.3083 ◽

2020 ◽

Vol 9 (1) ◽

pp. 29-42

Author(s):

Sabrine Mannai ◽

Hayfa Jabnoun-Khiareddine ◽

Bouzid Nasraoui ◽

Mejda Daami-Remadi

Keyword(s):

Bacillus Pumilus ◽

Pythium Ultimum ◽

Positive Control ◽

Dual Culture ◽

Damping Off ◽

Plant Weight ◽

Dual Culture Assay ◽

Emergence Percentage

Pythium ultimum is a common soilborne pathogen causing serious losses of pepper seedlings in nurseries and few weeks post-planting. Two pepper associated-P. ultimum isolates (P1 and P2) were shown pathogenic to pepper cv. Altar causing post-emergence damping-off with P2 isolate being the most aggressive. Fungal and bacterial antagonists have been evaluated in vitro and in vivo for their ability to suppress P. ultimum. In dual culture assay, Trichoderma harzianum, T. viride and Gliocladium virens inhibited pathogen radial growth by 18.54, 17.52 and 15.24%, respectively, relative to control, while none of the tested bacteria was shown able to significantly inhibit pathogen growth. However, drastic changes in pathogen hyphae expressed as strong lysis, the formation of mycelial cords and mycoparasitism have been observed. Pepper seeds treated with fungal antagonists’ conidial suspensions showed 60, 50 and 60% less pre-emergence damping-off infections, respectively, compared to the positive control. When tested as root dipping, only G. virens resulted in 40% reduced post-emergence damping-off. An improved seedlings fresh weight, by 79.31 and 76%, was respectively induced by G. virens-, and T. viride-based treatments while an increment of 27.58, 25.33 and 22.22 % was recorded following treatments with G. virens, T. viride and T. harzianum, relative to the positive control. The majority of tested bacterial isolates, applied as a seed treatment, had significantly improved the emergence percentage of inoculated seedlings as compared to control with Burkholderia glathei isolate 35 being the most efficient. When applied as root dipping, reduction of post-emergence damping-off ranged between 40 and 100% with Pseudomonas aureofaciens isolate 314 being the most effective agent. Seedlings treated with P. aureofaciens (314) and Bacillus pumilus (420) showed 35.38 and 28.51% higher heights, respectively. Plant weight was enhanced by 73.06, 61.18, 77.39, 61.8 and 67.93% over control following treatments with P. aureofaciens isolates 314 and 31, Bacillus pumilus 420, P. fluorescens and P. putida 227.

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Assessing hydrothermal treatment and antagonistic yeasts combination for mango anthracnose control

Acta Agrícola y Pecuaria ◽

10.30973/aap/2021.7.0071012 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

María de Jesús Ochoa-Rosas ◽

Ernestina Valadez-Moctezuma ◽

Gustavo Mena-Nevarez ◽

Teodoro Espinosa-Solares ◽

Artemio Pérez-López

Keyword(s):

Hydrothermal Treatment ◽

In Vitro Assays ◽

Harvest Management ◽

Postharvest Diseases ◽

Post Harvest ◽

Almost All ◽

Imagej Software ◽

Production Areas

Anthracnose caused by Colletotrichum gloeosporioides is the main post-harvest disease in mango in almost all production areas in the world. Hydrothermal treatment (HT: 46.1 °C for 70 min) and antagonism of yeasts Pichia guilliermondii, Candida oleophila and Candida quercitrusa were evaluated in vitro and in vivo to determine the most appropriate post-harvest management of anthracnose in "Ataulfo" mango. The pathogen C. gloeosporioides and the yeasts were evaluated at concentrations of 105 conidia ml-1 and 108 cells ml-1, respectively. The growth areas of the pathogen subjected to HT and in interaction with the yeasts were evaluated with ImageJ software. Subsequently, the most effective strains and the HT were evaluated in "Ataulfo" mango inoculated with C. gloeosporioides and the severity of the anthracnose was determined. In the in vitro assays, P. guilliermondii strain CDBB-932 was the most effective (18.6%) in controlling pathogen growth, presenting an inhibition halo that the rest of the yeasts did not show. In the in vivo assays, the combination of HT and C. quercitrusa (strain 42) was the most effective (96.8%) in the control of anthracnose. The hydrothermal treatment in combination with yeasts could be implemented preventative control of the postharvest diseases.

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Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502009000400021 ◽

2009 ◽

Vol 45 (4) ◽

pp. 759-766 ◽

Cited By ~ 13

Author(s):

Edith Cristina Laignier Cazedey ◽

Flávia Chiva Carvalho ◽

Flávia Angélica Másquio Fiorentino ◽

Maria Palmira Daflon Gremião ◽

Hérida Regina Nunes Salgado

Keyword(s):

Chemical Properties ◽

Alternative Methods ◽

In Vivo Studies ◽

In Vitro Assays ◽

Promising Alternative ◽

Animal Protection ◽

In Vivo Tests ◽

Pain And Suffering

Tests in animals are used as models in toxicological and investigative studies. However, such tests have been considered inhumane because they can cause pain and suffering to experimental animals, while these methods can often be subjective. Protests calling for animal protection have questioned the effectiveness of in vivo tests and suggest the introduction of alternative, in vitro methods. International organizations, such as the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the National Institute of Health (NIH), the Organization for Economic Co-operation and Development (OECD), that regulate and develop new alternative animal models, have indicated the running of preliminary assays and execution of sequential tests, which consider physical-chemical properties and data of in vitro assays, before performing in vivo studies. Towards this background, the objective of the present article was to select promising alternative methods such as Corrositex®, BCOP and HET-CAM, intended to refine or replace the use of animals and reduce their suffering.

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Potencial de extratos etanólicos de propólis e extratos aquosos de plantas espontâneas no controle de doenças pós-colheita do morango

Revista Verde de Agroecologia e Desenvolvimento Sustentável ◽

10.18378/rvads.v11i5.4175 ◽

2016 ◽

Vol 11 (1) ◽

pp. 57

Author(s):

Gabriela Silva Moura ◽

Jonas Marcelo Jaski ◽

Gilmar Franzener

Keyword(s):

Antifungal Activity ◽

Botrytis Cinerea ◽

Gray Mold ◽

Alternative Methods ◽

Wild Plants ◽

Rhizopus Nigricans ◽

Post Harvest ◽

Control Water

A cultura do morangueiro é severamente acometida por várias doenças, dentre elas o mofo cinzento, causado por Botrytis cinerea é considerada a doença mais severa na pós-colheita. Visando reduzir o uso de fungicidas sintéticos, vem sendo realizadas pesquisas propondo a utilização de métodos alternativos de controle de patógenos pós-colheita envolvendo a utilização de extratos vegetais, uso de biofungicidas e óleos essenciais. Assim, o presente trabalho teve como objetivo avaliar o potencial de diferentes extratos de própolis e plantas espontâneas no controle de podridão pós-colheita causada pelo fungo B. cinerea em morangos. Para avaliar a atividade antifúngica direta dos extratos etanólico de própolis e extratos aquosos de plantas espontâneas sobre B. cinerea, foi realizado o experimento in vitro, utilizando-se os tratamentos própolis verde 0,5%; própolis verde 2,5%; própolis marrom 0,5%; própolis marrom 2,5%; língua-de-vaca 10%; assa-peixe 10%; rubim 10%; tansagem 10%; testemunha (água). As medições do diâmetro das colônias foram iniciadas 48, 72 e 96 horas após a instalação do experimento. No experimento in vivo os frutos foram imersos nos tratamentos descritos acima. Após cinco dias avaliou-se a incidência e severidade da doença mofo cinzento e das doenças pós-colheita como antracnose e podridão de Rhizopus que apareceram no experimento. Utilizou-se o delineamento experimental inteiramente casualizado (DIC) com quatro e cinco repetições para o ensaio in vitro e in vivo, respectivamente. Os resultados mostram que os extratos etanólicos de própolis verde e marrom a 2,5% apresentaram in vitro e in vivo atividade antifúngica a B. cinerea e Rhizopus nigricans, respectivamente.Potential of propolis extracts and extracts etanol spontaneous plants aqueous in control of diseases of strawberry post-harvestAbstract: The strawberry crop is severely affected by various diseases, including gray mold, caused by Botrytis cinerea is considered the most severe disease in post-harvest fruit. To reduce the use of synthetic fungicides, has been carried out research proposing the use of alternative methods of control postharvest pathogens involving the use of plant extracts, use of biofungicides, essential oils among others. Thus, this study aimed to evaluate the potential of different propolis extracts and wild plants in the control of post-harvest rot caused by the fungus Botrytis cinerea in strawberries. To evaluate the direct antifungal activity of ethanolic extracts of propolis and aqueous extracts of wild plants of B. cinerea, the in vitro experiment was performed, using treatments propolis 0.5%; propolis 2.5%; brown propolis 0.5%; brown propolis 2.5%; control (water + alcohol 2%); cow tongue 10%; assa-fish 10%; rubim 10%; tansagem 10%; control (water). Measurements of the diameter of the colonies were started 48, 72 and 96 hours after installation of the experiment. Conducted the in vivo experiment in which the fruits of strawberry plants were immersed in the treatments described above. After five days we evaluated the incidence and severity of gray mold disease and post-harvest diseases such as anthracnose and Rhizopus rot appearing in the experiment. We used a completely randomized design (CRD) with four and five replicates for the in vitro assay and in vivo, respectively. The results show that ethanol extracts of green and brown propolis 2.5% presented in vitro and in vivo antifungal activity to B. cinerea and Rhizopus nigricans respectively.

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Evaluation of in-vivo animal and in-vitro models for prediction of dermal absorption in man

Human & Experimental Toxicology ◽

10.1177/0960327107085826 ◽

2008 ◽

Vol 27 (4) ◽

pp. 281-288 ◽

Cited By ~ 18

Author(s):

I Jakasa ◽

S Kezic

Keyword(s):

Animal Models ◽

Human Skin ◽

Predictive Value ◽

Percutaneous Absorption ◽

In Vitro Models ◽

Alternative Methods ◽

In Vitro Assays ◽

Rat Skin

Risk assessment of dermal exposure to chemicals requires percutaneous absorption data to link the external exposure to the systemic uptake. The most reliable data on percutaneous absorption are obtained from in-vivo human volunteer studies. In addition to ethical constrains, the conduct of these studies is not feasible for the large number of industrial chemicals in use today. Therefore, there is an increasing need for alternative methods to determine percutaneous absorption such as in-vitro assays and methods performed in vivo in experimental animals. In this article, recent comparative in-vitro and in-vivo studies on percutaneous absorption have been addressed with emphasis on the factors that may affect the predictive value of the in-vitro models. Furthermore, the use of animal models, in particular the rat skin, in prediction of percutaneous absorption in the human skin has been reviewed. In-vitro assays showed to be largely influenced by the experimental circumstances, such as type and thickness of the skin, receptor fluid, and the way in which percutaneous absorption is calculated. Rat skin showed consistently to be more permeable than human skin. However, the difference between human and rat skin does not show a consistent pattern between chemicals hampering prediction of human percutaneous absorption. To increase predictive value of in-vitro and animal models, the influence of experimental factors on the percutaneous absorption should be systematically investigated by comparison with human in-vivo data, resulting in more prescriptive guidelines.

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A in Vivo Assay for Standardizing Heparin Preparations

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646810 ◽

1979 ◽

Vol 41 (03) ◽

pp. 576-582

Author(s):

A R Pomeroy

Keyword(s):

In Vitro Assays ◽

British Pharmacopoeia ◽

In Vitro Method ◽

Standard Preparation ◽

Reliable Estimation ◽

Assay Procedure ◽

Accuracy And Precision ◽

Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

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Potentially Thrombogenic Materials in Factor IX Concentrates

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647857 ◽

1975 ◽

Vol 33 (03) ◽

pp. 617-631 ◽

Cited By ~ 47

Author(s):

H. S Kingdon ◽

R. L Lundblad ◽

J. J Veltkamp ◽

D. L Aronson

Keyword(s):

Human Plasma ◽

Antithrombin Iii ◽

Factor Ix ◽

In Vitro Assays ◽

Substantial Agreement ◽

Coefficient Of Correlation

SummaryFactor IX concentrates manufactured from human plasma and intended for therapeutic infusion in man have been suspected for some time of being potentially thrombogenic. In the current studies, assays were carried out in vitro and in vivo for potentially thrombogenic materials. It was possible to rank the various materials tested according to the amount of thrombogenic material detected. For concentrates not containing heparin, there was substantial agreement between the in vivo and in vitro assays, with a coefficient of correlation of 0.77. There was no correlation between the assays for thrombogenicity and the antithrombin III content. We conclude that many presently available concentrates of Factor IX contain substantial amounts of potentially thrombogenic enzymes, and that this fact must be considered in arriving at the decision whether or not to use them therapeutically.

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An Attempt at Biological Control of Blossom Blight of Rose Caused by Botrytis cinerea Using some Local Trichoderma spp. Strains

Acta Phytopathologica et Entomologica Hungarica ◽

10.1556/038.55.2020.010 ◽

2020 ◽

Vol 55 (1) ◽

pp. 27-34

Author(s):

G. Zadehdabagh ◽

K. Karimi ◽

M. Rezabaigi ◽

F. Ajamgard

Keyword(s):

Botrytis Cinerea ◽

Mycelial Growth ◽

Limiting Factor ◽

Dual Culture ◽

Trichoderma Spp ◽

Khuzestan Province ◽

Inhibitory Potential ◽

Cut Rose

The northern of Khuzestan province in Iran is mainly considered as one of the major areas of miniature rose production. Blossom blight caused by Botrytis cinerea has recently become a serious limiting factor in rose production in pre and post-harvest. In current study, an attempt was made to evaluate the inhibitory potential of some local Trichoderma spp. strains against B. cinerea under in vitro and in vivo conditions. The in vitro results showed that all Trichoderma spp. strains were significantly able to reduce the mycelial growth of the pathogen in dual culture, volatile and non-volatile compounds tests compared with control, with superiority of T. atroviride Tsafi than others. Under in vivo condition, the selected strain of T. atroviride Tsafi had much better performance than T. harzianum IRAN 523C in reduction of disease severity compared with the untreated control. Overall, the findings of this study showed that the application of Trichoderma-based biocontrol agents such as T. atroviride Tsafi can be effective to protect cut rose flowers against blossom blight.

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