High-throughput technique – targeted LC-MS/MS method to measure enterolactone "a biomarker of healthy lifestyle" for epidemiological investigation and clinical diagnosis

2016 ◽  
Author(s):  
Natalja Nørskov ◽  
Cecilie Kyrø ◽  
Anja Olsen ◽  
Anne Tjønneland ◽  
Knud Erik Knudsen
Keyword(s):  
Clinical Diagnosis ◽  
High Throughput ◽  
Healthy Lifestyle ◽  
Epidemiological Investigation

scholarly journals Epidemiological Distribution and Subtype Analysis of Premenstrual Dysphoric Disorder Syndromes and Symptoms Based on TCM Theories

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Mingqi Qiao ◽  
Peng Sun ◽  
Haijun Wang ◽  
Yang Wang ◽  
Xianghong Zhan ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Clinical Diagnosis ◽  
Premenstrual Dysphoric Disorder ◽  
Diagnosis And Treatment ◽  
Epidemiological Investigation ◽  
Different Types

We performed an epidemiological investigation of subjects with premenstrual dysphoric disorder (PMDD) to identify the clinical distribution of the major syndromes and symptoms. The pathogenesis of PMDD mainly involves the dysfunction of liver conveyance and dispersion. Excessive liver conveyance and dispersion are associated with liver-qi invasion syndrome, while insufficient liver conveyance and dispersion are expressed as liver-qi depression syndrome. Additionally, a nonconditional logistic regression was performed to analyze the symptomatic features of liver-qi invasion and liver-qi depression. As a result of this analysis, two subtypes of PMDD are proposed, namely, excessive liver conveyance and dispersion (liver-qi invasion syndrome) and insufficient liver conveyance and dispersion (liver-qi depression syndrome). Our findings provide an epidemiological foundation for the clinical diagnosis and treatment of PMDD based on the identification of different types.

High-throughput sequencing of the immune repertoire in oncology: Applications for clinical diagnosis, monitoring, and immunotherapies

2018 ◽  
Vol 416 ◽  
pp. 42-56 ◽  
Author(s):  
Baixin Ye ◽  
Daniel Smerin ◽  
Qingping Gao ◽  
Chunsheng Kang ◽  
Xiaoxing Xiong
Keyword(s):  
Clinical Diagnosis ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Immune Repertoire

scholarly journals Typing of Legionella isolates during an epidemiological investigation can be helpful but also misleading: an example from Greece

2006 ◽  
Vol 11 (34) ◽  
Author(s):  
E N Velonakis ◽  
S A Uldum ◽  
P Giakkoupi ◽  
S Loukoussias ◽  
S S Nielsen ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Working Group ◽  
Epidemiological Investigation ◽  
European Working Group ◽  
European Working

The guidelines of the European Working Group for Legionella Infections (EWGLI) [1] are mainly based on epidemiological criteria, because clinical diagnosis of the infection is seldom based on the isolation of this bacterium from the patient

scholarly journals Clinical diagnosis of single-gene disorders and chromosomal abnormalities based on BGISEQ-500 platform

2019 ◽  
Author(s):  
Yanqiu Liu ◽  
Liangwei Mao ◽  
Xiaoming Wei ◽  
Jianfen Man ◽  
Wenqian Zhang ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
Clinical Diagnosis ◽  
High Throughput ◽  
Copy Number ◽  
Chromosomal Abnormalities ◽  
Genomic Copy Number ◽  
Genomic Copy ◽  
Monogenic Diseases ◽  
Number Variation ◽  
Genomic Copy Number Variation

AbstractMost of the variation in the human genome is a single nucleotide variation (SNV) based on a single base or small fragment insertions and deletions and genomic copy number variation (CNV). Both types of mutations are involved in many human diseases. Such diseases often have complex clinical symptoms and difficult clinical diagnosis, so an effective detection method is needed to help clinical diagnosis and prevent birth defects. With the development of sequencing technology, the method of chip capture combined with high-throughput sequencing has been extensively used because of its high throughput, high accuracy, high speed and low cost. This study designed a chip that captures the coding region of 3043 genes associated with 4013 monogenic diseases. In addition, 148 chromosomal abnormalities can be identified by setting targets in specific regions. Compared with the whole exon chip, the chip can detect 4013 monogenic diseases and 148 chromosomal abnormalities at a lower cost, including SNV, intra-gene CNV and genomic copy number variation. This study utilized a strategy of combining the BGISEQ500 sequencing platform with the chip to identify 102 disease-associated mutations in 63 patients, 69 of which were new mutations. The evaluation test results also show that this combination complies with the requirements of clinical testing and has good clinical application value.

scholarly journals Targeted DNA Methylation Analysis by High-Throughput Sequencing in Hepatic Carcinoma Tissue and Adjacent Normal Tissues

2020 ◽  
Author(s):  
Guangmou Zhang ◽  
Huigen Feng ◽  
Zhiqing Yuan
Keyword(s):  
Dna Methylation ◽  
Liver Cancer ◽  
Clinical Diagnosis ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Methylation Analysis ◽  
Hepatic Carcinoma ◽  
Sequencing Technology ◽  
The Relationship ◽  
Dna Methylation Analysis

Abstract Background: The relationship between epigenetic abnormalities and tumorigenesi has been investigated in the past decade and made major advances, particularly the abnormal expression of small RNAs, DNA methylation, and histone modification in cancer. In many tumor-related studies, the regulatory changes in DNA methylation during cancer development and the development of resistance to anticancer drugs have show that DNA methylation can be used as a biomarker for cancer diagnosis and concomitant diagnosis, but there is a lack of clinically useful biomarkers associated with hepatic carcinoma. Using high-throughput sequencing technology, appropriate testing and validation can be carried out in large samples. The relationship between DNA methylation and tumor development can be explored, contributing to clinical diagnosis and personalized treatment of hepatic carcinoma. Methods: In this study, we implemented and evaluated the effectiveness of high-throughput sequencing for DNA methylation analysis in hepatic carcinoma. For the relationship between DNA methylation and gene expression, Pearson correlation analysis was used to evaluate the correlation. Twenty-five isolated genomic regions were amplified by PCR using bisulfite-transformed liver cancer tissue (Ca) and paracancer tissue (T) as template DNA. PCR final product sequence information was obtained by sequence analysis using Illumina Hiseq/Miseq platform. Results: The average depth of coverage across all amplicons was 30,548 for T and 29,346 for Ca, with a maximum of 3,675 at the ARID1A amplicon and a minimum of 65 at the PTEN amplicon. Methylation spectra were obtained for each genomic locus of the two groups of samples, and the results showed that methylation was significantly different at the X target loci and slightly different at the Y target loci. Cluster analysis showed that all T tissues were clustered in one group (except tissues T2 and T3), while Ca tissues were clustered on the other side. The results showed that DNA methylation at the loci may be closely related to liver cancer, providing references for the research and development of biomarkers in clinical diagnosis. Conclusions: The study demonstrates that high-throughput sequencing technology is a powerful and cost-effective method for methylation analysis of target DNA in cancer tissues.

Study on erythrocytes by SEM photogrammetry (SEMP) technique

1990 ◽  
Vol 48 (3) ◽  
pp. 724-725
Author(s):  
Tong Wensheng ◽  
Lu Lianhuang ◽  
Zhang Zhijun
Keyword(s):  
Quantitative Analysis ◽  
Cell Membrane ◽  
Clinical Diagnosis ◽  
Human Body ◽  
Blood Cells ◽  
Three Dimensional ◽  
Normal Person ◽  
Blood Disease ◽  
Computation Technique ◽  
Important Basis

This is a combined study of two diffirent branches, photogrammetry and morphology of blood cells. The three dimensional quantitative analysis of erythrocytes using SEMP technique, electron computation technique and photogrammetry theory has made it possible to push the study of mophology of blood cells from LM, TEM, SEM to a higher stage, that of SEM P. A new path has been broken for deeply study of morphology of blood cells.In medical view, the abnormality of the quality and quantity of erythrocytes is one of the important changes of blood disease. It shows the abnormal blood—making function of the human body. Therefore, the study of the change of shape on erythrocytes is the indispensable and important basis of reference in the clinical diagnosis and research of blood disease.The erythrocytes of one normal person, three PNH Patients and one AA patient were used in this experiment. This research determines the following items: Height;Length of two axes (long and short), ratio; Crevice in depth and width of cell membrane; Circumference of erythrocytes; Isoline map of erythrocytes; Section map of erythrocytes.

Sign in / Sign up

Export Citation Format

Share Document