scholarly journals Survey and first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea, Turkey

2021 ◽  
Author(s):  
Ulku Karaman ◽  
Zeynep Koloren ◽  
Panagiotis Karanis
Keyword(s):  
Water Resources ◽  
Black Sea ◽  
Water Samples ◽  
Water Sources ◽  
Spring Water ◽  
The Black Sea ◽  
First Report ◽  
Natural Spring Water ◽  
Acanthamoeba Species ◽  
Natural Spring

Abstract Infection with Acanthamoeba spp. may result in granulomatous amoebic encephalitis and Acanthamoeba keratitis. Water is an important habitat where Acanthamoeba species thrive. Therefore, studying the occurrence of this free-living amoeba in water sources will help understand the infection dynamics. The aim of the study was to survey and report on the presence of Acanthamoeba spp. in water resources from the Ordu and Giresun provinces in Black Sea. Acanthamoeba spp. was found in 1/17 natural spring water samples from Ordu and in 2/18 from Giresun. Acanthamoeba species were not detected in any of the investigated tap water samples. Sequencing of the (SSU) rDNA gene resulted in the identification of haplotype I (Acanthamoeba genotype: KJ094684). T4 (8.6%) was the only isolated genotype in both Ordu and Giresun provinces. This is the first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea. The occurrence of Acanthamoeba species in natural spring water sources should be considered as a potential risk for human infection, especially to high-risk populations.

Acanthamoeba spp. in river water samples from the Black Sea region, Turkey

2020 ◽  
Vol 18 (2) ◽  
pp. 186-199 ◽  
Author(s):  
İlknur Koyun ◽  
Zeynep Kolören ◽  
Ülkü Karaman ◽  
Amalia Tsiami ◽  
Panagiotis Karanis
Keyword(s):  
Black Sea ◽  
Water Samples ◽  
Tap Water ◽  
Acanthamoeba Keratitis ◽  
The Black Sea ◽  
Free Living ◽  
Black Sea Region ◽  
River Waters ◽  
Free Living Amoeba ◽  
Acanthamoeba Species

Abstract The present study aims to investigate the occurrence of free living amoeba (FLA) in water resources (rivers and tap water) in Samsun in the Black Sea. The presence of Acanthamoeba spp. was confirmed in 98 of 192 water samples collected from 32 sites of Samsun province (Samsun centre, Terme, Carsamba, Tekkekoy, Bafra) by PCR. Acanthamoeba spp. were found in 15/36 river samples from Samsun, in 58/90 from Terme, in 12/30 from Carsamba, in 7/18 from Tekkekoy and in 6/18 from Bafra. No Acanthamoeba species were detected in tap water samples. The highest rate in river waters contaminated with Acanthamoeba species was in Terme followed by Samsun centre (41.7%), Carsamba (40%), Tekkekoy (38.9%) and Bafra districts (33.3%), respectively. The result of the subsequent sequence analysis showed Haplotype I (A. triangularis) in 5%, Haplotype II (A. polyphaga) in 29.6%, Haplotype III (Acanthamoeba spp.) in 62% and Haplotype IV (A. lenticulata) in 3%. The most common genotype was Acanthamoeba T4 (Acanthamoeba spp., A. polyphaga, A. triangularis) and T5 genotype was also found in 3%. The T4 genotype is the most common genotype associated with Acanthamoeba keratitis (AK) worldwide; therefore, humans and animals living in the area are at risk after contact with such waters.

SITE-SPECIFIC EQUATIONS OF STATE FOR COASTAL SEA AREAS AND INLAND WATER BODIES

2017 ◽  
Author(s):  
Natalia Andrulionis ◽  
Natalia Andrulionis ◽  
Ivan Zavialov ◽  
Ivan Zavialov ◽  
Elena Kovaleva ◽  
...  
Keyword(s):  
Equation Of State ◽  
Black Sea ◽  
Water Samples ◽  
Equations Of State ◽  
Sea Water ◽  
Ionic Composition ◽  
Water Bodies ◽  
Aral Sea ◽  
The Black Sea ◽  
Coastal Sea

This article presents a new method of laboratory density determination and construction equations of state for marine waters with various ionic compositions and salinities was developed. The validation of the method was performed using the Ocean Standard Seawater and the UNESCO thermodynamic equation of state (EOS-80). Density measurements of water samples from the Aral Sea, the Black Sea and the Issyk-Kul Lake were performed using a high-precision laboratory density meter. The obtained results were compared with the density values calculated for the considered water samples by the EOS-80 equation. It was shown that difference in ionic composition between Standard Seawater and the considered water bodies results in significant inaccuracies in determination of water density using the EOS-80 equation. Basing on the laboratory measurements of density under various salinity and temperature values we constructed a new equation of state for the Aral Sea and the Black Sea water samples and estimated errors for their coefficients.

Oxygen, Hydrogen, Boron and Lithium Isotope Data of a Natural Spring Water with an Extreme Composition: A Fluid from the Dehydrating Slab?

2017 ◽  
Vol 23 (5-6) ◽  
pp. 299-313 ◽  
Author(s):  
Tiziano Boschetti ◽  
Lorenzo Toscani ◽  
Paola Iacumin ◽  
Enricomaria Selmo
Keyword(s):  
Spring Water ◽  
Lithium Isotope ◽  
Isotope Data ◽  
Natural Spring Water ◽  
Natural Spring

scholarly journals First Report of Enteromyxum leei (Myxozoa) in the Black Sea in a Potential Reservoir Host Chromis chromis

2014 ◽  
Vol 49 (2) ◽  
pp. 57-60 ◽  
Author(s):  
Ahmet ^|^Ouml;zer ◽  
T^|^uuml;rkay ^|^Ouml;zt^|^uuml;rk ◽  
Hakan ^|^Ouml;zkan ◽  
Arzu ^|^Ccedil;am
Keyword(s):  
Black Sea ◽  
Reservoir Host ◽  
The Black Sea ◽  
First Report ◽  
Potential Reservoir ◽  
Enteromyxum Leei

scholarly journals Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp.

2015 ◽  
Vol 12 (7) ◽  
pp. 7430-7443 ◽  
Author(s):  
Stefan Panaiotov ◽  
Ivan Simeonovski ◽  
Victoria Levterova ◽  
Ventzislav Karamfilov ◽  
Nadia Brankova ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Black Sea ◽  
Water Samples ◽  
The Black Sea ◽  
Mycobacterium Spp

scholarly journals Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia

2010 ◽  
Vol 76 (6) ◽  
pp. 1996-2001 ◽  
Author(s):  
Chris A. Whitehouse ◽  
Carson Baldwin ◽  
Rangarajan Sampath ◽  
Lawrence B. Blyn ◽  
Rachael Melton ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Black Sea ◽  
Water Samples ◽  
The Black Sea ◽  
Aquatic Environments ◽  
Ionization Mass ◽  
Freshwater Lakes ◽  
Total Water ◽  
Soviet Republic ◽  
Pathogenic Vibrio

ABSTRACT The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.

scholarly journals Rural Drinking Water Quality Status in Central Development Region, Nepal: A Comparative Study of Spring water and Ground water

2012 ◽  
Vol 9 ◽  
pp. 52-56
Author(s):  
Bishnu Pandey ◽  
Suman Shakya
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Ground Water ◽  
Water Samples ◽  
Water Sources ◽  
Spring Water ◽  
Drinking Water Quality ◽  
Development Region ◽  
Water Quality Status ◽  
Quality Status

This study assesses the rural drinking water quality status in Central Development Region of Nepal. With a total of 250 samples collected from 15 districts of the region, drinking water quality of spring water and ground water representing hill and Terai (lowland) regions were tested and compared for their physicochemical parameters and faecal coliform contamination.None of the spring samples as well as ground water samples violated National Drinking Water Standards (NDWS) for electrical conductivity (EC), total dissolved solids (TDS), total suspended solids (TSS), appearance, chloride and nitrate. Similarly none violated the standards for total hardness (TH) indicating soft nature of the water. The spring samples were within the NDWS for manganese (Mn) and iron (Fe) whereas 15.4% and 39.0% of the ground water samples violated the standards for manganese and iron, respectively. Gravity water is found to be more alkaline than ground water. Faecal coliforms were the most problematic in both types of sources followed by Ammonia (NH3) and pH in spring sources and by iron, Mn, pH and ammonia in ground water sources, respectively. Spring sources were more contaminated by bacteria than ground water sources. Correlation and regression analysis revealed highly significant correlations between EC and TDS (r=0.979) and between CaH and TH (r=0.988) in ground water suggesting that aquifer chemistry of ground water to be mainly controlled by EC, TDS, TH, and CaH. Similarly, highly significant correlations were found between the following pairs in gravity water: EC and TDS (r=0.983), TA and TDS(r=0.853), CaH and TDS (r=0.912), TH and TDS (r=0.955), EC and CaH (r=0.898), and between CaH and TH (r=0.951).DOI: http://dx.doi.org/10.3126/hn.v9i0.7074 Hydro Nepal Vol.9 July 2011 52-56

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