Acanthamoeba spp. in river water samples from the Black Sea region, Turkey

2020 ◽  
Vol 18 (2) ◽  
pp. 186-199 ◽  
Author(s):  
İlknur Koyun ◽  
Zeynep Kolören ◽  
Ülkü Karaman ◽  
Amalia Tsiami ◽  
Panagiotis Karanis
Keyword(s):  
Black Sea ◽  
Water Samples ◽  
Tap Water ◽  
Acanthamoeba Keratitis ◽  
The Black Sea ◽  
Free Living ◽  
Black Sea Region ◽  
River Waters ◽  
Free Living Amoeba ◽  
Acanthamoeba Species

Abstract The present study aims to investigate the occurrence of free living amoeba (FLA) in water resources (rivers and tap water) in Samsun in the Black Sea. The presence of Acanthamoeba spp. was confirmed in 98 of 192 water samples collected from 32 sites of Samsun province (Samsun centre, Terme, Carsamba, Tekkekoy, Bafra) by PCR. Acanthamoeba spp. were found in 15/36 river samples from Samsun, in 58/90 from Terme, in 12/30 from Carsamba, in 7/18 from Tekkekoy and in 6/18 from Bafra. No Acanthamoeba species were detected in tap water samples. The highest rate in river waters contaminated with Acanthamoeba species was in Terme followed by Samsun centre (41.7%), Carsamba (40%), Tekkekoy (38.9%) and Bafra districts (33.3%), respectively. The result of the subsequent sequence analysis showed Haplotype I (A. triangularis) in 5%, Haplotype II (A. polyphaga) in 29.6%, Haplotype III (Acanthamoeba spp.) in 62% and Haplotype IV (A. lenticulata) in 3%. The most common genotype was Acanthamoeba T4 (Acanthamoeba spp., A. polyphaga, A. triangularis) and T5 genotype was also found in 3%. The T4 genotype is the most common genotype associated with Acanthamoeba keratitis (AK) worldwide; therefore, humans and animals living in the area are at risk after contact with such waters.

scholarly journals Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 276
Author(s):  
Kawish Iqbal ◽  
Sumayah Abdelnasir Osman Abdalla ◽  
Ayaz Anwar ◽  
Kanwal Muhammad Iqbal ◽  
Muhammad Raza Shah ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Amphotericin B ◽  
Central Nervous System Infection ◽  
Acanthamoeba Castellanii ◽  
Acanthamoeba Keratitis ◽  
Free Living ◽  
Granulomatous Amoebic Encephalitis ◽  
Nano Drug Delivery ◽  
Free Living Amoeba ◽  
Acanthamoeba Species

The pathogenic free-living amoeba, Acanthamoeba castellanii, is responsible for a rare but deadly central nervous system infection, granulomatous amoebic encephalitis and a blinding eye disease called Acanthamoeba keratitis. Currently, a combination of biguanides, amidine, azoles and antibiotics are used to manage these infections; however, the host cell cytotoxicity of these drugs remains a challenge. Furthermore, Acanthamoeba species are capable of transforming to the cyst form to resist chemotherapy. Herein, we have developed a nano drug delivery system based on iron oxide nanoparticles conjugated with isoniazid, which were further loaded with amphotericin B (ISO-NPs-AMP) to cause potent antiamoebic effects against Acanthamoeba castellanii. The IC50 of isoniazid conjugated with magnetic nanoparticles and loaded with amphotericin B was found to be 45 μg/mL against Acanthamoeba castellanii trophozoites and 50 μg/mL against cysts. The results obtained in this study have promising implications in drug discovery as these nanomaterials exhibited high trophicidal and cysticidal effects, as well as limited cytotoxicity against rat and human cells.

scholarly journals Survey and first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea, Turkey

2021 ◽  
Author(s):  
Ulku Karaman ◽  
Zeynep Koloren ◽  
Panagiotis Karanis
Keyword(s):  
Water Resources ◽  
Black Sea ◽  
Water Samples ◽  
Water Sources ◽  
Spring Water ◽  
The Black Sea ◽  
First Report ◽  
Natural Spring Water ◽  
Acanthamoeba Species ◽  
Natural Spring

Abstract Infection with Acanthamoeba spp. may result in granulomatous amoebic encephalitis and Acanthamoeba keratitis. Water is an important habitat where Acanthamoeba species thrive. Therefore, studying the occurrence of this free-living amoeba in water sources will help understand the infection dynamics. The aim of the study was to survey and report on the presence of Acanthamoeba spp. in water resources from the Ordu and Giresun provinces in Black Sea. Acanthamoeba spp. was found in 1/17 natural spring water samples from Ordu and in 2/18 from Giresun. Acanthamoeba species were not detected in any of the investigated tap water samples. Sequencing of the (SSU) rDNA gene resulted in the identification of haplotype I (Acanthamoeba genotype: KJ094684). T4 (8.6%) was the only isolated genotype in both Ordu and Giresun provinces. This is the first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea. The occurrence of Acanthamoeba species in natural spring water sources should be considered as a potential risk for human infection, especially to high-risk populations.

Acanthamoeba Species in Tap Water, Egypt

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Ahmad Z Al-Herrawy ◽  
Mohamed A Marouf ◽  
Mahmoud A. Gad
Keyword(s):  
Water Samples ◽  
Tap Water ◽  
Pcr Analysis ◽  
Pulmonary Infections ◽  
Clinical Syndromes ◽  
Acanthamoeba Species ◽  
Amebic Encephalitis ◽  
Acanthamoeba Culbertsoni ◽  
Autumn And Winter

Genus Acanthamoeba causes 3 clinical syndromes amebic keratitis, granulomatous amebic encephalitis and disseminated granulomatous amebic disease (eg, sinus, skin and pulmonary infections). A total of 144 tap water samples were collected from Giza governorate, Egypt. Samples were processed for detection of Acanthamoeba species using non-nutrient agar (NNA) and were incubated at 30oC. The isolates of Acanthamoeba were identified to species level based on the morphologic criteria. Molecular characterization of the Acanthamoeba isolates to genus level was performed by using PCR. The obtained results showed that the highest occurrence percentage of Acanthamoeba species in water samples was observed in summer season (38.9%), then it decreased to be 30.6% in spring and 25% in each of autumn and winter. PCR analysis showed that 100% of 43 Acanthamoeba morphologically positive samples were positive by genus specific primer. In the present study eight species of Acanthamoeba can be morphologically recognized namely Acanthamoeba triangularis, Acanthamoeba echinulata, Acanthamoeba astronyxis, Acanthamoeba comandoni, Acanthamoeba griffini, Acanthamoeba culbertsoni, Acanthamoeba quina and Acanthamoeba lenticulata. In conclusion, the most common Acanthamoeba species in tap water was Acanthamoeba comandoni

SITE-SPECIFIC EQUATIONS OF STATE FOR COASTAL SEA AREAS AND INLAND WATER BODIES

2017 ◽  
Author(s):  
Natalia Andrulionis ◽  
Natalia Andrulionis ◽  
Ivan Zavialov ◽  
Ivan Zavialov ◽  
Elena Kovaleva ◽  
...  
Keyword(s):  
Equation Of State ◽  
Black Sea ◽  
Water Samples ◽  
Equations Of State ◽  
Sea Water ◽  
Ionic Composition ◽  
Water Bodies ◽  
Aral Sea ◽  
The Black Sea ◽  
Coastal Sea

This article presents a new method of laboratory density determination and construction equations of state for marine waters with various ionic compositions and salinities was developed. The validation of the method was performed using the Ocean Standard Seawater and the UNESCO thermodynamic equation of state (EOS-80). Density measurements of water samples from the Aral Sea, the Black Sea and the Issyk-Kul Lake were performed using a high-precision laboratory density meter. The obtained results were compared with the density values calculated for the considered water samples by the EOS-80 equation. It was shown that difference in ionic composition between Standard Seawater and the considered water bodies results in significant inaccuracies in determination of water density using the EOS-80 equation. Basing on the laboratory measurements of density under various salinity and temperature values we constructed a new equation of state for the Aral Sea and the Black Sea water samples and estimated errors for their coefficients.

Obsidian Exchange Networks in Prehistoric Anatolia: New Data from the Black Sea Region

Paléorient ◽  
2013 ◽  
Vol 39 (2) ◽  
pp. 173-182
Author(s):  
Bleda S. Düring ◽  
Bernard Gratuze
Keyword(s):  
Black Sea ◽  
The Black Sea ◽  
Exchange Networks ◽  
Black Sea Region

The referendum as an institution in the Black Sea Region: Does it signal the rise or collapse of democracy?

2019 ◽  
Vol 17 (4) ◽  
pp. 161-185
Author(s):  
Małgorzata Podolak
Keyword(s):  
Black Sea ◽  
Direct Democracy ◽  
The Black Sea ◽  
Moral Issues ◽  
Black Sea Region ◽  
Positive Effects ◽  
The Subject ◽  
Democratic Systems ◽  
Important State ◽  
Constitutional Changes

Views on the institution of direct democracy have changed during the period of democratic transition. The various advantages and positive effects of direct democracy have been confirmed by the practice of some democratic countries. Its educational and political activation value for society was also noted, without which civil society cannot form. The referendum is especially treated as the purest form of correlation between the views of society and the decisions of its representatives. In a situation where two representative bodies are present – the parliament and the president – a referendum is considered a means of resolving disputes between them in important state affairs. The referendum is nowadays becoming more than just a binding or consultative opinion on a legislative act, especially a constitution. First and foremost, it is important to see the extension of the type and scope of issues that are subject to direct voting. Apart from the traditional, i.e., constitutional changes, polarising issues that raise considerable emotion have become the subject of referenda. Problems of this type include, in particular, moral issues, membership in international organisations, and so-called ‘New Policy’. This article presents the role and importance of the referendum as an institution shaping the democratic systems of the Black Sea Region.

Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates

2014 ◽  
Vol 59 (3) ◽  
Author(s):  
Monika Derda ◽  
Agnieszka Wojtkowiak-Giera ◽  
Edward Hadaś
Keyword(s):  
Genetic Markers ◽  
Tap Water ◽  
Correct Diagnosis ◽  
Specific Marker ◽  
Pcr Assay ◽  
Free Living ◽  
Detection And Identification ◽  
Granulomatous Amoebic Encephalitis ◽  
Free Living Amoeba ◽  
Treatment Of Infection

AbstractAcanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic and prognostic factor for the treatment of infection is a quick and correct diagnosis of amoebae strains. The aim of our study was to develop a rapid method for detection and identification of pathogenic Acanthamoeba spp. strains from diagnostic material collected from water. In this study we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP, Nelson) used for identification of pathogenic Acanthamoeba spp. strains isolated in water sources in Poland, Iceland and Sweden. Our results demonstrated the presence of pathogenic Acanthamoeba strains in tap water. PCR assay appeared to be a more rapid and sensitive method to detect the presence of amoebae than the limited conventional techniques. Based on our observations, we can confirm that the use of four out of five genetic markers (Aca 16S, Ac 6/210, JDP, GP, Nelson) may be helpful in identification of Acanthamoeba spp. strains, but only one Aca 16S primer pair is a highly specific marker that distinguishes between pathogenic strains of Acanthamoeba and other free-living amoeba families.

Analysis of potential seismic sources of tsunamis in the Black Sea region, using data from various catalogues

2021 ◽  
Author(s):  
Emil Oynakov ◽  
Liliya Dimitrova ◽  
Lyubka Pashova ◽  
Dragomir Dragomirov
Keyword(s):  
Black Sea ◽  
Scientific Evidence ◽  
Active Faults ◽  
Active Regions ◽  
The Black Sea ◽  
Earthquake Catalogues ◽  
Crimean Peninsula ◽  
Black Sea Region ◽  
Global Science ◽  
Using Data

<p>Low-laying territories along the Black Sea coastal line are more vulnerable to the possible high (long) waves due to tsunami events caused by strong earthquakes in the active seismic regions. Historically, such events are rare in the Black Sea region, despite some scientific evidence of tsunamis and their recordings through continuous sea-level observations with tide gauges built in certain places along the coast. This study analyses seismic data derived from different international earthquake catalogues - NEIC, ISC, EMSC, IDC and Bulgarian national catalogue (1981 - 2019). A catalogue of earthquakes within the period covering the historical to the contemporary seismicity with magnitudes M ≥ 3 is compiled. The data are processed applying the software package ZMAP, developed by Stefan Wiemer (http://www.seismo.ethz.ch/en/research-and-teaching/products-software/software/ZMAP/index.html). The catalogues' completeness is calculated to assess the reliability of the historical data needed to assess the risk of rare tsunami events. The prevailing part of the earthquakes' epicentres are in the seismically active regions of Shabla, the Crimean peninsula, the east and southeast coast of the Black Sea forming six main clusters, which confirmed previous studies in the region. In these areas, several active and potentially active faults, which can generate tsunamigenic seismic events, are recognized.</p><p><strong>Acknowledgements: </strong>The authors would like to thank the Bulgarian National Science Fund for co-funding the research under the Contract КП-СЕ-КОСТ/8, 25.09.2020, which is carried out within framework of COST Action 18109 “Accelerating Global science In Tsunami HAzard and Risk analysis” (AGITHAR; https://www.agithar.uni-hamburg.de/).</p>

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