scholarly journals Selection of Reliable Reference Genes for Gene Expression Studies in the Biofuel Plant Jatropha curcas Using Real-Time Quantitative PCR

2013 ◽  
Vol 14 (12) ◽  
pp. 24338-24354 ◽  
Author(s):  
Lu Zhang ◽  
Liang-Liang He ◽  
Qian-Tang Fu ◽  
Zeng-Fu Xu
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Quantitative Pcr ◽  
Jatropha Curcas ◽  
Reference Genes ◽  
Real Time Quantitative Pcr ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time PCR in Tung Tree during Seed Development

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43084 ◽  
Author(s):  
Xiaojiao Han ◽  
Mengzhu Lu ◽  
Yicun Chen ◽  
Zhiyong Zhan ◽  
Qinqin Cui ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Seed Development ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Tung Tree ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

Screening of reference genes using real-time quantitative PCR for gene expression studies inNeoseiulus barkeriHughes (Acari: Phytoseiidae)

2018 ◽  
Vol 109 (4) ◽  
pp. 443-452 ◽  
Author(s):  
C. Wang ◽  
J. Yang ◽  
Q. Pan ◽  
S. Yu ◽  
R. Luo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Genes ◽  
Developmental Stages ◽  
Quantitative Polymerase Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
The Stability ◽  
Suitable Reference

AbstractA stable reference gene is a key prerequisite for accurate assessment of gene expression. At present, the real-time reverse transcriptase quantitative polymerase chain reaction has been widely used in the analysis of gene expression in a variety of organisms.Neoseiulus barkeriHughes (Acari: Phytoseiidae) is a major predator of mites on many important economically crops. Until now, however, there are no reports evaluating the stability of reference genes in this species. In view of this, we used GeNorm, NormFinder, BestKeeper, and RefFinder software tools to evaluate the expression stability of 11 candidate reference genes in developmental stages and under various abiotic stresses. According to our results, β-ACTandHsp40were the top two stable reference genes in developmental stages. TheHsp60andHsp90were the most stable reference genes in various acaricides stress. For alterations in temperature,Hsp40and α-TUBwere the most suitable reference genes. About UV stress,EF1α and α-TUBwere the best choice, and for the different prey stress, β-ACTand α-TUBwere best suited. In normal conditions, the β-ACT and α-TUB were the two of the highest stable reference genes to respond to all kinds of stresses. The current study provided a valuable foundation for the further analysis of gene expression inN. barkeri.

scholarly journals Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR

2010 ◽  
Vol 187 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Katherine Nelissen ◽  
Karen Smeets ◽  
Monique Mulder ◽  
Jerome J.A. Hendriks ◽  
Marcel Ameloot
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document